Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella

Salmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to a...

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Autores: Paytubi Casabona, Sònia, Cansado, Cintia, Madrid Xufré, Cristina, Balsalobre Parra, Carlos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/117751
Acceso en línea:https://hdl.handle.net/2445/117751
Access Level:acceso abierto
Palabra clave:Biofilms
Salmonel·la
Salmonella
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spelling Nutrient composition promotes switching between pellicle and bottom biofilm in SalmonellaPaytubi Casabona, SòniaCansado, CintiaMadrid Xufré, CristinaBalsalobre Parra, CarlosBiofilmsSalmonel·laBiofilmsSalmonellaSalmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to antimicrobials and disinfectants. It has been described that there are two crucial biofilm promoting factors in Salmonella: curli and cellulose. The expression of both factors is coordinately regulated by the transcriptional regulator CsgD. Most biofilm studies of Salmonella have been performed by growing bacteria in low osmolarity rich medium and low temperature (25°C). In such conditions, the biofilm is formed at the air-liquid interface (pellicle biofilm). Remarkably, when Salmonella grow in minimal medium, biofilm formation switches from the air-liquid interface to the solid-liquid interface (bottom biofilm). In this report, the switching between pellicle and bottom biofilm has been characterized. Our data indicate that curli, but not cellulose, is crucial for the formation of both kinds of biofilms. In minimal medium, conditions promoting formation of bottom biofilm, a high transcriptional expression of csgD and consequently of the genes involved in the synthesis of curli and cellulose was detected. The nutritional status of the cells seems to be pivotal for the spatial distribution of the biofilms formed. When bacteria is growing in minimal medium the addition of amino acids downregulates the expression of csgB and causes the switch between bottom and pellicle biofilm. The crosstalk between general metabolism and biofilm formation is also highlighted by the fact that the metabolic sensor cAMP modulates the type of biofilm generated by Salmonella. Moreover, cAMP regulates transcriptional expression of csgD and stimulates pellicle biofilm formation, suggesting that the physiological conditions define the type of biofilm formed by Salmonella. The consequences of the switching between pellicle and bottom biofilm during either infection or survival in natural environments remain undercover.Frontiers Media2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/117751Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fmicb.2017.02160Frontiers in Microbiology, 2017, vol. 8, p. 2160https://doi.org/10.3389/fmicb.2017.02160cc-by (c) Paytubi, Sonia et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1177512026-05-27T06:46:51Z
dc.title.none.fl_str_mv Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
title Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
spellingShingle Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
Paytubi Casabona, Sònia
Biofilms
Salmonel·la
Biofilms
Salmonella
title_short Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
title_full Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
title_fullStr Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
title_full_unstemmed Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
title_sort Nutrient composition promotes switching between pellicle and bottom biofilm in Salmonella
dc.creator.none.fl_str_mv Paytubi Casabona, Sònia
Cansado, Cintia
Madrid Xufré, Cristina
Balsalobre Parra, Carlos
author Paytubi Casabona, Sònia
author_facet Paytubi Casabona, Sònia
Cansado, Cintia
Madrid Xufré, Cristina
Balsalobre Parra, Carlos
author_role author
author2 Cansado, Cintia
Madrid Xufré, Cristina
Balsalobre Parra, Carlos
author2_role author
author
author
dc.subject.none.fl_str_mv Biofilms
Salmonel·la
Biofilms
Salmonella
topic Biofilms
Salmonel·la
Biofilms
Salmonella
description Salmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to antimicrobials and disinfectants. It has been described that there are two crucial biofilm promoting factors in Salmonella: curli and cellulose. The expression of both factors is coordinately regulated by the transcriptional regulator CsgD. Most biofilm studies of Salmonella have been performed by growing bacteria in low osmolarity rich medium and low temperature (25°C). In such conditions, the biofilm is formed at the air-liquid interface (pellicle biofilm). Remarkably, when Salmonella grow in minimal medium, biofilm formation switches from the air-liquid interface to the solid-liquid interface (bottom biofilm). In this report, the switching between pellicle and bottom biofilm has been characterized. Our data indicate that curli, but not cellulose, is crucial for the formation of both kinds of biofilms. In minimal medium, conditions promoting formation of bottom biofilm, a high transcriptional expression of csgD and consequently of the genes involved in the synthesis of curli and cellulose was detected. The nutritional status of the cells seems to be pivotal for the spatial distribution of the biofilms formed. When bacteria is growing in minimal medium the addition of amino acids downregulates the expression of csgB and causes the switch between bottom and pellicle biofilm. The crosstalk between general metabolism and biofilm formation is also highlighted by the fact that the metabolic sensor cAMP modulates the type of biofilm generated by Salmonella. Moreover, cAMP regulates transcriptional expression of csgD and stimulates pellicle biofilm formation, suggesting that the physiological conditions define the type of biofilm formed by Salmonella. The consequences of the switching between pellicle and bottom biofilm during either infection or survival in natural environments remain undercover.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/117751
url https://hdl.handle.net/2445/117751
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2017.02160
Frontiers in Microbiology, 2017, vol. 8, p. 2160
https://doi.org/10.3389/fmicb.2017.02160
dc.rights.none.fl_str_mv cc-by (c) Paytubi, Sonia et al., 2017
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Paytubi, Sonia et al., 2017
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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