Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows

The present study describes the virological and serological profiles of PCV2 vaccinated (V) and non-vaccinated (NV) piglet subpopulations coming from V and NV sows in a PCV2 subclinically infected farm. Four hundred seventy-six piglets born from V or NV sows were further subdivided in a total of fou...

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Autores: Fraile Sauce, Lorenzo José, Segalés, Joaquim, Ticó, G, López-Soria, Sergio, Valero, O., Nofrarías, Miquel, Huerta, E., Llorens, A., López-Jiménez, R., Pérez, D., Sibila, Marina
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/64977
Acceso en línea:https://doi.org/10.1016/j.prevetmed.2015.02.017
http://hdl.handle.net/10459.1/64977
Access Level:acceso abierto
Palabra clave:Porcine circovirus type 2 (PCV2)
Vaccination
Sow
Piglet
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spelling Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sowsFraile Sauce, Lorenzo JoséSegalés, JoaquimTicó, GLópez-Soria, SergioValero, O.Nofrarías, MiquelHuerta, E.Llorens, A.López-Jiménez, R.Pérez, D.Sibila, MarinaPorcine circovirus type 2 (PCV2)VaccinationSowPigletThe present study describes the virological and serological profiles of PCV2 vaccinated (V) and non-vaccinated (NV) piglet subpopulations coming from V and NV sows in a PCV2 subclinically infected farm. Four hundred seventy-six piglets born from V or NV sows were further subdivided in a total of four groups: NV sows–NV pigs (NV–NV), NV sows–V pigs (NV–V); V sows–NV pigs (V–NV) and V sows–V pigs (V–V). Seventy-five pigs were randomly selected at the beginning of the trial from each group and they were bled at 4, 8, 12, 16, 21 and 25 weeks of age. All animals included in the trial were weighed at 4 and 25 weeks of age and their average daily weight gain (ADWG) was calculated. Serum samples obtained at different time points were used to assess PCV2 infection (viremia) and the level of antibodies by means of immunoperoxidase monolayer assay (IPMA) against this pathogen. IPMA titers (classified in high, medium or low) and PCR results (positive or negative) were analyzed using a multiple correspondence and K-means cluster analysis. According to these tests, animals included in the study were classified into the following four clusters: (1) 93 piglets that were viremic mainly from 12 to 25 weeks of age and with PCV2 antibody titers increasing over time; (2) 75 piglets with late PCV2 infection and seroconversion (later than 16 weeks of age); (3) 26 piglets with high but decreasing PCV2 antibody titers and low percentages of PCV2 PCR positive serum samples; and (4) 105 piglets with medium and high IPMA titers throughout the trial and sporadic PCR positive samples. The defined subpopulations of piglets were observed in all experimental groups (NV–NV, NV–V, V–NV and V–V) although in variable percentages. Thus, animals from clusters 1 and 2 belonged mainly to the NV–NV and V–NV groups and animals from clusters 3 and 4 were distributed mainly into the NV–V and V–V groups. Finally, the ADWG of pigs belonging to clusters 3 and 4 was significantly higher (p = 0.02) than that of pigs belonging to clusters 1 and 2. Within each cluster, no statistically significant differences were found in ADWG between treatment groups.The field study was supported by MSD Animal Health and the experimental design as well as the results were discussed together with scientists at MSD Animal Health and CReSA.Elsevier2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1016/j.prevetmed.2015.02.017http://hdl.handle.net/10459.1/64977reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.prevetmed.2015.02.017Preventive Veterinary Medicine, 2015, vol. 119, núm. 3-4, p. 153-161cc-by-nc-nd (c) Elsevier, 2015info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/esoai:repositori.udl.cat:10459.1/649772026-06-24T12:42:17Z
dc.title.none.fl_str_mv Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
title Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
spellingShingle Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
Fraile Sauce, Lorenzo José
Porcine circovirus type 2 (PCV2)
Vaccination
Sow
Piglet
title_short Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
title_full Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
title_fullStr Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
title_full_unstemmed Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
title_sort Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows
dc.creator.none.fl_str_mv Fraile Sauce, Lorenzo José
Segalés, Joaquim
Ticó, G
López-Soria, Sergio
Valero, O.
Nofrarías, Miquel
Huerta, E.
Llorens, A.
López-Jiménez, R.
Pérez, D.
Sibila, Marina
author Fraile Sauce, Lorenzo José
author_facet Fraile Sauce, Lorenzo José
Segalés, Joaquim
Ticó, G
López-Soria, Sergio
Valero, O.
Nofrarías, Miquel
Huerta, E.
Llorens, A.
López-Jiménez, R.
Pérez, D.
Sibila, Marina
author_role author
author2 Segalés, Joaquim
Ticó, G
López-Soria, Sergio
Valero, O.
Nofrarías, Miquel
Huerta, E.
Llorens, A.
López-Jiménez, R.
Pérez, D.
Sibila, Marina
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Porcine circovirus type 2 (PCV2)
Vaccination
Sow
Piglet
topic Porcine circovirus type 2 (PCV2)
Vaccination
Sow
Piglet
description The present study describes the virological and serological profiles of PCV2 vaccinated (V) and non-vaccinated (NV) piglet subpopulations coming from V and NV sows in a PCV2 subclinically infected farm. Four hundred seventy-six piglets born from V or NV sows were further subdivided in a total of four groups: NV sows–NV pigs (NV–NV), NV sows–V pigs (NV–V); V sows–NV pigs (V–NV) and V sows–V pigs (V–V). Seventy-five pigs were randomly selected at the beginning of the trial from each group and they were bled at 4, 8, 12, 16, 21 and 25 weeks of age. All animals included in the trial were weighed at 4 and 25 weeks of age and their average daily weight gain (ADWG) was calculated. Serum samples obtained at different time points were used to assess PCV2 infection (viremia) and the level of antibodies by means of immunoperoxidase monolayer assay (IPMA) against this pathogen. IPMA titers (classified in high, medium or low) and PCR results (positive or negative) were analyzed using a multiple correspondence and K-means cluster analysis. According to these tests, animals included in the study were classified into the following four clusters: (1) 93 piglets that were viremic mainly from 12 to 25 weeks of age and with PCV2 antibody titers increasing over time; (2) 75 piglets with late PCV2 infection and seroconversion (later than 16 weeks of age); (3) 26 piglets with high but decreasing PCV2 antibody titers and low percentages of PCV2 PCR positive serum samples; and (4) 105 piglets with medium and high IPMA titers throughout the trial and sporadic PCR positive samples. The defined subpopulations of piglets were observed in all experimental groups (NV–NV, NV–V, V–NV and V–V) although in variable percentages. Thus, animals from clusters 1 and 2 belonged mainly to the NV–NV and V–NV groups and animals from clusters 3 and 4 were distributed mainly into the NV–V and V–V groups. Finally, the ADWG of pigs belonging to clusters 3 and 4 was significantly higher (p = 0.02) than that of pigs belonging to clusters 1 and 2. Within each cluster, no statistically significant differences were found in ADWG between treatment groups.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.prevetmed.2015.02.017
http://hdl.handle.net/10459.1/64977
url https://doi.org/10.1016/j.prevetmed.2015.02.017
http://hdl.handle.net/10459.1/64977
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.prevetmed.2015.02.017
Preventive Veterinary Medicine, 2015, vol. 119, núm. 3-4, p. 153-161
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier, 2015
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/es
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier, 2015
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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