The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria
[ENG]Introduction: Increasing evidence supports the role of gut microbiota in many aspects of human health, including immune, metabolic and neurobehavioral traits. Several studies have focused on how different components of the diet, such as polyphenols, can modulate the composition and function of...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:dnet:gredos______::cfe1cd0c39d380df3000dea1978fe6c2 |
| Acceso en línea: | http://hdl.handle.net/10366/171628 |
| Access Level: | acceso abierto |
| Palabra clave: | gut microbiota epicatechin quercetin probiotics Lactobacillus Bifidobacterium Enterococcus Quercetin quercetina |
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The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteriaAyuda Durán, María BegoñaSánchez Hernández, EvaGonzález Manzano, SusanaSantos Buelga, CelestinoGonzález Paramás, Ana M.gut microbiotaepicatechinquercetinprobioticsLactobacillusBifidobacteriumEnterococcusBifidobacteriumLactobacillusQuercetinEnterococcusquercetinaLactobacillusBifidobacteriumEnterococcus[ENG]Introduction: Increasing evidence supports the role of gut microbiota in many aspects of human health, including immune, metabolic and neurobehavioral traits. Several studies have focused on how different components of the diet, such as polyphenols, can modulate the composition and function of the gut microbiota leading to health benefits. Methods: The effects on the resistance against thermally induced oxidative stress of C. elegans grown in the presence of flavonoids (quercetin or epicatechin) and fed different probiotic strains, namely Lactobacillus plantarum CLC17, Bifidobacterium longum NCIMB 8809 and Enterococcus faecium CECT 410, were explored. Results: Feeding C. elegans with the assayed bacteria in the absence of flavonoids did not significantly affect body size and fertility of the worms neither improve their resistance against oxidative stress compared to E. coli controls. However, increased resistance to stress was found when C. elegans was cultivated in the presence of both L. plantarum and flavonoids, but not with B. longum or E. faecium. An exploratory study revealed the presence of glycosylated and sulfated metabolites together with the aglycone in worms treated with quercetin and fed any of the different assayed LAB strains. However, in the assays with epicatechin a differential metabolite, tentatively identified as 5-(4′-hydroxyphenyl)-γ-valerolactone 3′-O-glucoside, was detected in the worms fed L. plantarum but not with the other bacteria. Conclusion: The obtained results indicated that the interactions bacteria/polyphenol play a key role in the effects produced in C. elegans regarding resistance against oxidative stress, although those effects cannot be only explained by the ability of bacteria to metabolize polyphenols, but other mechanisms should also be involved.The GIP-USAL was financially supported by the Spanish Ministerio de Ciencia e Innovación (Project PID2019-106167RB-I00/AEI/10.13039/501100011033), Consejería de Educación (Project SA093P20), and the Strategic Research Programs for Units of Excellence from Junta de Castilla y León (ref. CLU-2018-04).https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.989427/full202620262022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/171628reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)EspañolAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:dnet:gredos______::cfe1cd0c39d380df3000dea1978fe6c22026-06-07T06:28:51Z |
| dc.title.none.fl_str_mv |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria |
| title |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria |
| spellingShingle |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria Ayuda Durán, María Begoña gut microbiota epicatechin quercetin probiotics Lactobacillus Bifidobacterium Enterococcus Bifidobacterium Lactobacillus Quercetin Enterococcus quercetina Lactobacillus Bifidobacterium Enterococcus |
| title_short |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria |
| title_full |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria |
| title_fullStr |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria |
| title_full_unstemmed |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria |
| title_sort |
The effects of polyphenols against oxidative stress in Caenorhabditis elegans are determined by coexisting bacteria |
| dc.creator.none.fl_str_mv |
Ayuda Durán, María Begoña Sánchez Hernández, Eva González Manzano, Susana Santos Buelga, Celestino González Paramás, Ana M. |
| author |
Ayuda Durán, María Begoña |
| author_facet |
Ayuda Durán, María Begoña Sánchez Hernández, Eva González Manzano, Susana Santos Buelga, Celestino González Paramás, Ana M. |
| author_role |
author |
| author2 |
Sánchez Hernández, Eva González Manzano, Susana Santos Buelga, Celestino González Paramás, Ana M. |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
gut microbiota epicatechin quercetin probiotics Lactobacillus Bifidobacterium Enterococcus Bifidobacterium Lactobacillus Quercetin Enterococcus quercetina Lactobacillus Bifidobacterium Enterococcus |
| topic |
gut microbiota epicatechin quercetin probiotics Lactobacillus Bifidobacterium Enterococcus Bifidobacterium Lactobacillus Quercetin Enterococcus quercetina Lactobacillus Bifidobacterium Enterococcus |
| description |
[ENG]Introduction: Increasing evidence supports the role of gut microbiota in many aspects of human health, including immune, metabolic and neurobehavioral traits. Several studies have focused on how different components of the diet, such as polyphenols, can modulate the composition and function of the gut microbiota leading to health benefits. Methods: The effects on the resistance against thermally induced oxidative stress of C. elegans grown in the presence of flavonoids (quercetin or epicatechin) and fed different probiotic strains, namely Lactobacillus plantarum CLC17, Bifidobacterium longum NCIMB 8809 and Enterococcus faecium CECT 410, were explored. Results: Feeding C. elegans with the assayed bacteria in the absence of flavonoids did not significantly affect body size and fertility of the worms neither improve their resistance against oxidative stress compared to E. coli controls. However, increased resistance to stress was found when C. elegans was cultivated in the presence of both L. plantarum and flavonoids, but not with B. longum or E. faecium. An exploratory study revealed the presence of glycosylated and sulfated metabolites together with the aglycone in worms treated with quercetin and fed any of the different assayed LAB strains. However, in the assays with epicatechin a differential metabolite, tentatively identified as 5-(4′-hydroxyphenyl)-γ-valerolactone 3′-O-glucoside, was detected in the worms fed L. plantarum but not with the other bacteria. Conclusion: The obtained results indicated that the interactions bacteria/polyphenol play a key role in the effects produced in C. elegans regarding resistance against oxidative stress, although those effects cannot be only explained by the ability of bacteria to metabolize polyphenols, but other mechanisms should also be involved. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2026 2026 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10366/171628 |
| url |
http://hdl.handle.net/10366/171628 |
| dc.language.none.fl_str_mv |
Español |
| language_invalid_str_mv |
Español |
| dc.rights.none.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.989427/full |
| publisher.none.fl_str_mv |
https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2022.989427/full |
| dc.source.none.fl_str_mv |
reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca instname:Universidad de Salamanca (USAL) |
| instname_str |
Universidad de Salamanca (USAL) |
| reponame_str |
GREDOS. Repositorio Institucional de la Universidad de Salamanca |
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GREDOS. Repositorio Institucional de la Universidad de Salamanca |
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15,81155 |