Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)

To examine the role of glucose-6-phosphatase (G6Pase) in glucose homeostasis in the diabeteslike experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of G6Pase expression at the enzyme activity an...

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Autores: Caseras Surribas, Anna, Metón Teijeiro, Isidoro, Vives, C., Egea Liria, Miriam, Fernández González, Felipe Javier, Vázquez Baanante, Ma. Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2002
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/33807
Acceso en línea:https://hdl.handle.net/2445/33807
Access Level:acceso abierto
Palabra clave:Enzimologia
Peixos
Regulació del metabolisme
Piscicultura
Orada
Expressió gènica
Glucocinasa
Nutrició animal
Enzymology
Fishes
Metabolic regulation
Pisciculture
Sparus aurata
Gene expression
Glucokinase
Animal nutrition
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spelling Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)Caseras Surribas, AnnaMetón Teijeiro, IsidoroVives, C.Egea Liria, MiriamFernández González, Felipe JavierVázquez Baanante, Ma. IsabelEnzimologiaPeixosRegulació del metabolismePisciculturaOradaExpressió gènicaGlucocinasaNutrició animalEnzymologyFishesMetabolic regulationPiscicultureSparus aurataGene expressionGlucokinaseAnimal nutritionTo examine the role of glucose-6-phosphatase (G6Pase) in glucose homeostasis in the diabeteslike experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of G6Pase expression at the enzyme activity and mRNA level in the liver of gilthead sea bream (Sparus aurata ). G6Pase expression increased in long-term starved or energy-restricted fish. In contrast to data reported for other fish species, short-term regulation of G6Pase expression was found in regularly fed S. aurata. G6Pase mRNA levels were lowest between 4 and 15 h after food intake, whereas minimal enzyme activity was observed 10-15 h postprandially. Alterations of plasma glucose levels affect G6Pase in mammals. However, the carbohydrate content of the diet did not affect hepatic expression of G6Pase in S. aurata, suggesting that a different molecular mechanism is involved in the control of G6Pase expression in fish. Although G6Pase was unaffected, highcarbohydrate low-protein diets increased glucokinase (GK) expression and thus allowed a metabolic adaptation favouring glycolysis over gluconeogenesis. Interestingly, only the nutritional conditions that promoted variations in the blood glucose levels resulted in changes in the hepatic expression of G6Pase. These findings indicate a concerted regulation of G6Pase and GK expression and suggest that the direction and rate of the glucose-glucose-6-phosphate substrate cycle flux is finely regulated in the liver of S. aurata, challenging the role attributed to deficient regulation of G6Pase or GK expression in the low ability of carnivorous fish to metabolize glucose.Cambridge University Press2013201320022013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/33807Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1079/BJN2002701British Journal of Nutrition, 2002, vol. 88, num. 6, p. 607-614http://dx.doi.org/10.1079/BJN2002701(c) Cambridge University Press, 2002info:eu-repo/semantics/openAccessoai:recercat.cat:2445/338072026-05-29T05:05:01Z
dc.title.none.fl_str_mv Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
title Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
spellingShingle Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
Caseras Surribas, Anna
Enzimologia
Peixos
Regulació del metabolisme
Piscicultura
Orada
Expressió gènica
Glucocinasa
Nutrició animal
Enzymology
Fishes
Metabolic regulation
Pisciculture
Sparus aurata
Gene expression
Glucokinase
Animal nutrition
title_short Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
title_full Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
title_fullStr Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
title_full_unstemmed Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
title_sort Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)
dc.creator.none.fl_str_mv Caseras Surribas, Anna
Metón Teijeiro, Isidoro
Vives, C.
Egea Liria, Miriam
Fernández González, Felipe Javier
Vázquez Baanante, Ma. Isabel
author Caseras Surribas, Anna
author_facet Caseras Surribas, Anna
Metón Teijeiro, Isidoro
Vives, C.
Egea Liria, Miriam
Fernández González, Felipe Javier
Vázquez Baanante, Ma. Isabel
author_role author
author2 Metón Teijeiro, Isidoro
Vives, C.
Egea Liria, Miriam
Fernández González, Felipe Javier
Vázquez Baanante, Ma. Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enzimologia
Peixos
Regulació del metabolisme
Piscicultura
Orada
Expressió gènica
Glucocinasa
Nutrició animal
Enzymology
Fishes
Metabolic regulation
Pisciculture
Sparus aurata
Gene expression
Glucokinase
Animal nutrition
topic Enzimologia
Peixos
Regulació del metabolisme
Piscicultura
Orada
Expressió gènica
Glucocinasa
Nutrició animal
Enzymology
Fishes
Metabolic regulation
Pisciculture
Sparus aurata
Gene expression
Glucokinase
Animal nutrition
description To examine the role of glucose-6-phosphatase (G6Pase) in glucose homeostasis in the diabeteslike experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of G6Pase expression at the enzyme activity and mRNA level in the liver of gilthead sea bream (Sparus aurata ). G6Pase expression increased in long-term starved or energy-restricted fish. In contrast to data reported for other fish species, short-term regulation of G6Pase expression was found in regularly fed S. aurata. G6Pase mRNA levels were lowest between 4 and 15 h after food intake, whereas minimal enzyme activity was observed 10-15 h postprandially. Alterations of plasma glucose levels affect G6Pase in mammals. However, the carbohydrate content of the diet did not affect hepatic expression of G6Pase in S. aurata, suggesting that a different molecular mechanism is involved in the control of G6Pase expression in fish. Although G6Pase was unaffected, highcarbohydrate low-protein diets increased glucokinase (GK) expression and thus allowed a metabolic adaptation favouring glycolysis over gluconeogenesis. Interestingly, only the nutritional conditions that promoted variations in the blood glucose levels resulted in changes in the hepatic expression of G6Pase. These findings indicate a concerted regulation of G6Pase and GK expression and suggest that the direction and rate of the glucose-glucose-6-phosphate substrate cycle flux is finely regulated in the liver of S. aurata, challenging the role attributed to deficient regulation of G6Pase or GK expression in the low ability of carnivorous fish to metabolize glucose.
publishDate 2002
dc.date.none.fl_str_mv 2002
2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/33807
url https://hdl.handle.net/2445/33807
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1079/BJN2002701
British Journal of Nutrition, 2002, vol. 88, num. 6, p. 607-614
http://dx.doi.org/10.1079/BJN2002701
dc.rights.none.fl_str_mv (c) Cambridge University Press, 2002
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Cambridge University Press, 2002
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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