Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials

The industrial use of enzymes generally necessitates their immobilization onto solid supports. The well-known high affinity of enzymes for metal-organic framework (MOF) materials, together with the great versatility of MOFs in terms of structure, composition, functionalization and synthetic approach...

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Detalles Bibliográficos
Autores: Molina Esquinas, Asunción, Gascón Pérez, Victoria, Sánchez Sánchez, Manuel, Blanco Martín, Rosa María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372648
Acceso en línea:http://hdl.handle.net/10261/372648
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113196964&doi=10.3390%2fcatal11081002&partnerID=40&md5=fa002649860701abdd9ff115112b849c
Access Level:acceso abierto
Palabra clave:Enz@MOF
Enzyme immobilization
Fe-BTC
In situ
Low leaching
NH2-MIL-53(Al)
One-step
Room temperature
Sustainable MOFs as supports
ZIF-8
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repository_id_str
spelling Sustainable one-pot immobilization of enzymes in/on metal-organic framework materialsMolina Esquinas, AsunciónGascón Pérez, VictoriaSánchez Sánchez, ManuelBlanco Martín, Rosa MaríaEnz@MOFEnzyme immobilizationFe-BTCIn situLow leachingNH2-MIL-53(Al)One-stepRoom temperatureSustainable MOFs as supportsZIF-8The industrial use of enzymes generally necessitates their immobilization onto solid supports. The well-known high affinity of enzymes for metal-organic framework (MOF) materials, together with the great versatility of MOFs in terms of structure, composition, functionalization and synthetic approaches, has led the scientific community to develop very different strategies for the immobilization of enzymes in/on MOFs. This review focuses on one of these strategies, namely, the one-pot enzyme immobilization within sustainable MOFs, which is particularly enticing as the resultant biocomposite Enzyme@MOFs have the potential to be: (i) prepared in situ, that is, in just one step; (ii) may be synthesized under sustainable conditions: with water as the sole solvent at room temperature with moderate pHs, etc.; (iii) are able to retain high enzyme loading; (iv) have negligible protein leaching; and (v) give enzymatic activities approaching that given by the corresponding free enzymes. Moreover, this methodology seems to be near-universal, as success has been achieved with different MOFs, with different enzymes and for different applications. So far, the metal ions forming the MOF materials have been chosen according to their low price, low toxicity and, of course, their possibility for generating MOFs at room temperature in water, in order to close the cycle of economic, environmental and energy sustainability in the synthesis, application and disposal life cycle. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.This research was funded by CSIC (2019AEP076). M.A.M. acknowledges Spanish MINECO for PhD student contract BES-2017-082077. English-writing style. The Government of Ireland Postdoctoral Fellowship-2015 GOIPD/2015/287 is gratefully acknowledged by V.G.-P.Peer reviewedMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas (España)Ministerio de Economía y Competitividad (España)Government of the United KingdomAgencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_dcae04bcPublisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/372648https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113196964&doi=10.3390%2fcatal11081002&partnerID=40&md5=fa002649860701abdd9ff115112b849creponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BES-2017-082077Catalystshttps://doi.org/10.3390/catal11081002Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3726482026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
title Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
spellingShingle Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
Molina Esquinas, Asunción
Enz@MOF
Enzyme immobilization
Fe-BTC
In situ
Low leaching
NH2-MIL-53(Al)
One-step
Room temperature
Sustainable MOFs as supports
ZIF-8
title_short Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
title_full Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
title_fullStr Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
title_full_unstemmed Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
title_sort Sustainable one-pot immobilization of enzymes in/on metal-organic framework materials
dc.creator.none.fl_str_mv Molina Esquinas, Asunción
Gascón Pérez, Victoria
Sánchez Sánchez, Manuel
Blanco Martín, Rosa María
author Molina Esquinas, Asunción
author_facet Molina Esquinas, Asunción
Gascón Pérez, Victoria
Sánchez Sánchez, Manuel
Blanco Martín, Rosa María
author_role author
author2 Gascón Pérez, Victoria
Sánchez Sánchez, Manuel
Blanco Martín, Rosa María
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Ministerio de Economía y Competitividad (España)
Government of the United Kingdom
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Enz@MOF
Enzyme immobilization
Fe-BTC
In situ
Low leaching
NH2-MIL-53(Al)
One-step
Room temperature
Sustainable MOFs as supports
ZIF-8
topic Enz@MOF
Enzyme immobilization
Fe-BTC
In situ
Low leaching
NH2-MIL-53(Al)
One-step
Room temperature
Sustainable MOFs as supports
ZIF-8
description The industrial use of enzymes generally necessitates their immobilization onto solid supports. The well-known high affinity of enzymes for metal-organic framework (MOF) materials, together with the great versatility of MOFs in terms of structure, composition, functionalization and synthetic approaches, has led the scientific community to develop very different strategies for the immobilization of enzymes in/on MOFs. This review focuses on one of these strategies, namely, the one-pot enzyme immobilization within sustainable MOFs, which is particularly enticing as the resultant biocomposite Enzyme@MOFs have the potential to be: (i) prepared in situ, that is, in just one step; (ii) may be synthesized under sustainable conditions: with water as the sole solvent at room temperature with moderate pHs, etc.; (iii) are able to retain high enzyme loading; (iv) have negligible protein leaching; and (v) give enzymatic activities approaching that given by the corresponding free enzymes. Moreover, this methodology seems to be near-universal, as success has been achieved with different MOFs, with different enzymes and for different applications. So far, the metal ions forming the MOF materials have been chosen according to their low price, low toxicity and, of course, their possibility for generating MOFs at room temperature in water, in order to close the cycle of economic, environmental and energy sustainability in the synthesis, application and disposal life cycle. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2021
dc.date.none.fl_str_mv 2021
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_dcae04bc
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/372648
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113196964&doi=10.3390%2fcatal11081002&partnerID=40&md5=fa002649860701abdd9ff115112b849c
url http://hdl.handle.net/10261/372648
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85113196964&doi=10.3390%2fcatal11081002&partnerID=40&md5=fa002649860701abdd9ff115112b849c
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BES-2017-082077
Catalysts
https://doi.org/10.3390/catal11081002
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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