Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence

Objective: To study how long-term elevated non-esterified fatty acid (NEFA) concentrations, typical in metabolic disorders such as obesity or type 2 diabetes, affect murine follicular development, follicle quality, and subsequent oocyte developmental competence in vitro. Design: Experimental study....

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Autores: Valckx, Sara, Van Hoeck, Veerle, Arias Álvarez, María, Maillo, Verónica, López-Cardona, Ángela P., Gutiérrez Adán, Alfonso, Berth, Mario, Cortvrindt, Rita, Bols, Peter E.J., Leroy, J.L.M.R.
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/102785
Acceso en línea:https://hdl.handle.net/20.500.14352/102785
Access Level:acceso abierto
Palabra clave:636.09
Metabolic disorder
Obesity
NEFA
Folliculogenesis
Oocyte developmental competence
Veterinaria
3109 Ciencias Veterinarias
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spelling Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competenceValckx, SaraVan Hoeck, VeerleArias Álvarez, MaríaMaillo, VerónicaLópez-Cardona, Ángela P.Gutiérrez Adán, AlfonsoBerth, MarioCortvrindt, RitaBols, Peter E.J.Leroy, J.L.M.R.636.09Metabolic disorderObesityNEFAFolliculogenesisOocyte developmental competenceVeterinaria3109 Ciencias VeterinariasObjective: To study how long-term elevated non-esterified fatty acid (NEFA) concentrations, typical in metabolic disorders such as obesity or type 2 diabetes, affect murine follicular development, follicle quality, and subsequent oocyte developmental competence in vitro. Design: Experimental study. Setting: In vitro culture setting. Animal(s): Female and male 13-day old, B6CBAF1 mice of proven fertility were sacrificed for harvesting ovaries and epididymal sperm, respectively. Intervention(s): Early secondary murine follicles were cultured in vitro in the presence of NEFAs until the antral stage (12 days). Treatments consisted of one or a mixture of NEFAs (stearic acid [SA], palmitic acid [PA], oleic acid [OA]) in physiological (basal) or pathological (high SA, high OA, high NEFA) concentrations. Main Outcome Measure(s): Follicular development; follicle and oocyte diameters; secretion of progesterone, estradiol, and inhibin B; and luteinized granulosa cell gene expression patterns were investigated. Oocytes from NEFA-exposed follicles were fertilized in vitro, and presumptive zygotes were cultured until the blastocyst stage. Result(s): Exposure to high SA reduced follicle diameters and day-12 antrum formation. Elevated NEFA concentrations changed luteinized granulosa cell messenger–ribonucleic acid abundance of genes related to energy/fatty acid/steroid metabolism, apoptosis, and oxidative stress. High NEFA and high SA treatments increased progesterone synthesis, compared with high OA follicles. Oocyte developmental competence was substantially reduced in oocytes retrieved from high OA–, high SA–, and high NEFA–exposed follicles compared with basal–treated follicles. Conclusion(s): This study showed, for the first time, that lipolysis-linked, elevated NEFA concentrations can potentially impair fertility, by altering follicular physiology and reducing oocyte developmental competence.ElsevierUniversidad Complutense de Madrid20142014-09-2320142014-09-23journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/102785reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1027852026-06-02T12:44:21Z
dc.title.none.fl_str_mv Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
title Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
spellingShingle Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
Valckx, Sara
636.09
Metabolic disorder
Obesity
NEFA
Folliculogenesis
Oocyte developmental competence
Veterinaria
3109 Ciencias Veterinarias
title_short Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
title_full Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
title_fullStr Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
title_full_unstemmed Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
title_sort Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence
dc.creator.none.fl_str_mv Valckx, Sara
Van Hoeck, Veerle
Arias Álvarez, María
Maillo, Verónica
López-Cardona, Ángela P.
Gutiérrez Adán, Alfonso
Berth, Mario
Cortvrindt, Rita
Bols, Peter E.J.
Leroy, J.L.M.R.
author Valckx, Sara
author_facet Valckx, Sara
Van Hoeck, Veerle
Arias Álvarez, María
Maillo, Verónica
López-Cardona, Ángela P.
Gutiérrez Adán, Alfonso
Berth, Mario
Cortvrindt, Rita
Bols, Peter E.J.
Leroy, J.L.M.R.
author_role author
author2 Van Hoeck, Veerle
Arias Álvarez, María
Maillo, Verónica
López-Cardona, Ángela P.
Gutiérrez Adán, Alfonso
Berth, Mario
Cortvrindt, Rita
Bols, Peter E.J.
Leroy, J.L.M.R.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Metabolic disorder
Obesity
NEFA
Folliculogenesis
Oocyte developmental competence
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
Metabolic disorder
Obesity
NEFA
Folliculogenesis
Oocyte developmental competence
Veterinaria
3109 Ciencias Veterinarias
description Objective: To study how long-term elevated non-esterified fatty acid (NEFA) concentrations, typical in metabolic disorders such as obesity or type 2 diabetes, affect murine follicular development, follicle quality, and subsequent oocyte developmental competence in vitro. Design: Experimental study. Setting: In vitro culture setting. Animal(s): Female and male 13-day old, B6CBAF1 mice of proven fertility were sacrificed for harvesting ovaries and epididymal sperm, respectively. Intervention(s): Early secondary murine follicles were cultured in vitro in the presence of NEFAs until the antral stage (12 days). Treatments consisted of one or a mixture of NEFAs (stearic acid [SA], palmitic acid [PA], oleic acid [OA]) in physiological (basal) or pathological (high SA, high OA, high NEFA) concentrations. Main Outcome Measure(s): Follicular development; follicle and oocyte diameters; secretion of progesterone, estradiol, and inhibin B; and luteinized granulosa cell gene expression patterns were investigated. Oocytes from NEFA-exposed follicles were fertilized in vitro, and presumptive zygotes were cultured until the blastocyst stage. Result(s): Exposure to high SA reduced follicle diameters and day-12 antrum formation. Elevated NEFA concentrations changed luteinized granulosa cell messenger–ribonucleic acid abundance of genes related to energy/fatty acid/steroid metabolism, apoptosis, and oxidative stress. High NEFA and high SA treatments increased progesterone synthesis, compared with high OA follicles. Oocyte developmental competence was substantially reduced in oocytes retrieved from high OA–, high SA–, and high NEFA–exposed follicles compared with basal–treated follicles. Conclusion(s): This study showed, for the first time, that lipolysis-linked, elevated NEFA concentrations can potentially impair fertility, by altering follicular physiology and reducing oocyte developmental competence.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-09-23
2014
2014-09-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/102785
url https://hdl.handle.net/20.500.14352/102785
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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