Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream

The influence of chronic stress, induced by food deprivation (FD) and/or high stocking density (HSD), was assessed on stress, vasotocinergic and isotocinergic pathways of the gilthead sea bream (Sparus aurata). Fish were randomly assigned to one of the following treatments: (1) fed at low stocking d...

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Autores: Skrzynska, Arleta K., Martos-Sitcha, Juan Antonio, Martínez-Rodríguez, Gonzalo, Mancera, Juan Miguel
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/190391
Acesso em linha:http://hdl.handle.net/10261/190391
Access Level:Acceso aberto
Palavra-chave:Arginine vasotocin
Food deprivation
Isotocin
Sparus aurata
Stocking density
Stress
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spelling Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea breamSkrzynska, Arleta K.Martos-Sitcha, Juan AntonioMartínez-Rodríguez, GonzaloMancera, Juan MiguelArginine vasotocinFood deprivationIsotocinSparus aurataStocking densityStressThe influence of chronic stress, induced by food deprivation (FD) and/or high stocking density (HSD), was assessed on stress, vasotocinergic and isotocinergic pathways of the gilthead sea bream (Sparus aurata). Fish were randomly assigned to one of the following treatments: (1) fed at low stocking density (LSD-F; 5 kg·m− 3); (2) fed at high stocking density (HSD-F, 40 kg·m− 3); (3) food-deprived at LSD (LSD-FD); and (4) food-deprived at HSD (HSD-FD). After 21 days, samples from plasma, liver, hypothalamus, pituitary and head-kidney were collected. Both stressors (FD and HSD) induced a chronic stress situation, as indicated by the elevated cortisol levels, the enhancement in corticotrophin releasing hormone (crh) expression and the down-regulation in corticotrophin releasing hormone binding protein (crhbp) expression. Changes in plasma and liver metabolites confirmed a metabolic adjustment to cope with energy demand imposed by stressors. Changes in avt and it gene expression, as well as in their specific receptors (avtrv1a, avtrv2 and itr) at central (hypothalamus and pituitary) and peripheral (liver and head-kidney) levels, showed that vasotocinergic and isotocinergic pathways are involved in physiological changes induced by FD or HSD, suggesting that different stressors are handled through different stress pathways in S. aurata.This study was funded by projects AGL2010-14876 (Ministry of Science and Innovation) and AGL2013-48835-C2-1-R (Spanish Ministry of Economy and Competitiveness, MINECO) awarded to J.M.M. J.A.M-S is currently funded by a Postdoctoral Research Fellow (Juan de la Cierva-Formación, Reference FJCI-2014-20161) from MINECO.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Martos-Sitcha, Juan Antonio [0000-0002-0151-7250]Martínez-Rodríguez, Gonzalo [0000-0003-3379-580X]Mancera, Juan Miguel [0000-0003-0751-5966]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/190391reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-48835-C2-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FJCI-2014-20161http://dx.doi.org/10.1016/j.cbpa.2017.10.012Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1903912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
title Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
spellingShingle Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
Skrzynska, Arleta K.
Arginine vasotocin
Food deprivation
Isotocin
Sparus aurata
Stocking density
Stress
title_short Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
title_full Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
title_fullStr Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
title_full_unstemmed Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
title_sort Unraveling vasotocinergic, isotocinergic and stress pathways after food deprivation and high stocking density in the gilthead sea bream
dc.creator.none.fl_str_mv Skrzynska, Arleta K.
Martos-Sitcha, Juan Antonio
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
author Skrzynska, Arleta K.
author_facet Skrzynska, Arleta K.
Martos-Sitcha, Juan Antonio
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
author_role author
author2 Martos-Sitcha, Juan Antonio
Martínez-Rodríguez, Gonzalo
Mancera, Juan Miguel
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Martos-Sitcha, Juan Antonio [0000-0002-0151-7250]
Martínez-Rodríguez, Gonzalo [0000-0003-3379-580X]
Mancera, Juan Miguel [0000-0003-0751-5966]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arginine vasotocin
Food deprivation
Isotocin
Sparus aurata
Stocking density
Stress
topic Arginine vasotocin
Food deprivation
Isotocin
Sparus aurata
Stocking density
Stress
description The influence of chronic stress, induced by food deprivation (FD) and/or high stocking density (HSD), was assessed on stress, vasotocinergic and isotocinergic pathways of the gilthead sea bream (Sparus aurata). Fish were randomly assigned to one of the following treatments: (1) fed at low stocking density (LSD-F; 5 kg·m− 3); (2) fed at high stocking density (HSD-F, 40 kg·m− 3); (3) food-deprived at LSD (LSD-FD); and (4) food-deprived at HSD (HSD-FD). After 21 days, samples from plasma, liver, hypothalamus, pituitary and head-kidney were collected. Both stressors (FD and HSD) induced a chronic stress situation, as indicated by the elevated cortisol levels, the enhancement in corticotrophin releasing hormone (crh) expression and the down-regulation in corticotrophin releasing hormone binding protein (crhbp) expression. Changes in plasma and liver metabolites confirmed a metabolic adjustment to cope with energy demand imposed by stressors. Changes in avt and it gene expression, as well as in their specific receptors (avtrv1a, avtrv2 and itr) at central (hypothalamus and pituitary) and peripheral (liver and head-kidney) levels, showed that vasotocinergic and isotocinergic pathways are involved in physiological changes induced by FD or HSD, suggesting that different stressors are handled through different stress pathways in S. aurata.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/190391
url http://hdl.handle.net/10261/190391
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-48835-C2-1-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FJCI-2014-20161
http://dx.doi.org/10.1016/j.cbpa.2017.10.012
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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