Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells

[EN] Mesenchymal stem cells (MSCs) osteogenic commitment before injection enhances bone regeneration therapy results. Piezoelectric stimulation may be an effective cue to promote MSCs pre-differentiation, and poly(vinylidene) fluoride (PVDF) cell culture supports, when combined with CoFe2O4 (CFO), o...

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Authors: Guillot-Ferriols, María Teresa, Tolosa, Laia, Costa, Carlos M., Lanceros-Méndez, Senentxu, García-Briega, María Inmaculada|||0000-0003-0811-2632, Gómez Ribelles, José Luís|||0000-0001-9099-0885, Gallego-Ferrer, Gloria|||0000-0002-2428-0903
Format: article
Publication Date:2022
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:riunet.upv.es:10251/193207
Online Access:https://riunet.upv.es/handle/10251/193207
Access Level:Open access
Keyword:Mesenchymal stem cells
Osteoblastogenesis
Piezoelectricity
Poly(vinylidene) fluoride
Hydrogel
MAQUINAS Y MOTORES TERMICOS
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oai_identifier_str oai:riunet.upv.es:10251/193207
network_acronym_str ES
network_name_str España
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dc.title.none.fl_str_mv Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
title Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
spellingShingle Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
Guillot-Ferriols, María Teresa
Mesenchymal stem cells
Osteoblastogenesis
Piezoelectricity
Poly(vinylidene) fluoride
Hydrogel
MAQUINAS Y MOTORES TERMICOS
title_short Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
title_full Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
title_fullStr Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
title_full_unstemmed Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
title_sort Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem Cells
dc.creator.none.fl_str_mv Guillot-Ferriols, María Teresa
Tolosa, Laia
Costa, Carlos M.
Lanceros-Méndez, Senentxu
García-Briega, María Inmaculada|||0000-0003-0811-2632
Gómez Ribelles, José Luís|||0000-0001-9099-0885
Gallego-Ferrer, Gloria|||0000-0002-2428-0903
author Guillot-Ferriols, María Teresa
author_facet Guillot-Ferriols, María Teresa
Tolosa, Laia
Costa, Carlos M.
Lanceros-Méndez, Senentxu
García-Briega, María Inmaculada|||0000-0003-0811-2632
Gómez Ribelles, José Luís|||0000-0001-9099-0885
Gallego-Ferrer, Gloria|||0000-0002-2428-0903
author_role author
author2 Tolosa, Laia
Costa, Carlos M.
Lanceros-Méndez, Senentxu
García-Briega, María Inmaculada|||0000-0003-0811-2632
Gómez Ribelles, José Luís|||0000-0001-9099-0885
Gallego-Ferrer, Gloria|||0000-0002-2428-0903
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Termodinámica Aplicada
Centro de Biomateriales e Ingeniería Tisular
Escuela Técnica Superior de Ingeniería Industrial
AGENCIA ESTATAL DE INVESTIGACION
Agencia Estatal de Investigación
European Regional Development Fund
Fundação para a Ciência e a Tecnologia, Portugal
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Mesenchymal stem cells
Osteoblastogenesis
Piezoelectricity
Poly(vinylidene) fluoride
Hydrogel
MAQUINAS Y MOTORES TERMICOS
topic Mesenchymal stem cells
Osteoblastogenesis
Piezoelectricity
Poly(vinylidene) fluoride
Hydrogel
MAQUINAS Y MOTORES TERMICOS
description [EN] Mesenchymal stem cells (MSCs) osteogenic commitment before injection enhances bone regeneration therapy results. Piezoelectric stimulation may be an effective cue to promote MSCs pre-differentiation, and poly(vinylidene) fluoride (PVDF) cell culture supports, when combined with CoFe2O4 (CFO), offer a wireless in vitro stimulation strategy. Under an external magnetic field, CFO shift and magnetostriction deform the polymer matrix varying the polymer surface charge due to the piezoelectric effect. To test the effect of piezoelectric stimulation on MSCs, our approach is based on a gelatin hydrogel with embedded MSCs and PVDF-CFO electroactive microspheres. Microspheres were produced by electrospray technique, favouring CFO incorporation, crystallisation in beta-phase (85%) and a crystallinity degree of around 55%. The absence of cytotoxicity of the 3D construct was confirmed 24 h after cell encapsulation. Cells were viable, evenly distributed in the hydrogel matrix and surrounded by microspheres, allowing local stimulation. Hydrogels were stimulated using a magnetic bioreactor, and no significant changes were observed in MSCs proliferation in the short or long term. Nevertheless, piezoelectric stimulation upregulated RUNX2 expression after 7 days, indicating the activation of the osteogenic differentiation pathway. These results open the door for optimising a stimulation protocol allowing the application of the magnetically activated 3D electroactive cell culture support for MSCs pre-differentiation before transplantation.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/193207
url https://riunet.upv.es/handle/10251/193207
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106000RB-C21 HIDROGELES BIOMIMETICOS IMPRIMIBLES CON PRESENTACION DE FACTORES DE CRECIMIENTO EFICIENTE PARA ESTUDIOS DE HEPATOTOXICIDAD DE ALTO RENDIMIENTO
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 AYUDA PARA CONTRATOS PREDOCTORALES PARA LA FORMACIÓN DE DOCTORES 2017 BES-2017-080398 BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACIÓN CELULAR EN INTERFACES CELULA-MATERIAL ELÉCTRICAMENTE ACTIVAS
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C41 MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLE
Fundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 CEEC 2020 2020.04028.CEECIND
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C43 DESARROLLO DE ANDAMIAJES BIOMIMETICOS ACTIVOS PARA EL ESTUDIO DE MICROENTORNO DE TUMOR EN OSTEOSARCOMA
Fundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 Financiamento Estratégico de Unidades de I&D UID%2FFIS%2F04650%2F2021
Fundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 Proyectos I&D FCT – PTDC PTDC%2FFIS-MAC%2F28157%2F2017
Fundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 COMPETE 2020 POCI (FEDER) POCI-01-0145-FEDER-007688
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
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spelling Magnetically Activated Piezoelectric 3D Platform Based on Poly(Vinylidene) Fluoride Microspheres for Osteogenic Differentiation of Mesenchymal Stem CellsGuillot-Ferriols, María TeresaTolosa, LaiaCosta, Carlos M.Lanceros-Méndez, SenentxuGarcía-Briega, María Inmaculada|||0000-0003-0811-2632Gómez Ribelles, José Luís|||0000-0001-9099-0885Gallego-Ferrer, Gloria|||0000-0002-2428-0903Mesenchymal stem cellsOsteoblastogenesisPiezoelectricityPoly(vinylidene) fluorideHydrogelMAQUINAS Y MOTORES TERMICOS[EN] Mesenchymal stem cells (MSCs) osteogenic commitment before injection enhances bone regeneration therapy results. Piezoelectric stimulation may be an effective cue to promote MSCs pre-differentiation, and poly(vinylidene) fluoride (PVDF) cell culture supports, when combined with CoFe2O4 (CFO), offer a wireless in vitro stimulation strategy. Under an external magnetic field, CFO shift and magnetostriction deform the polymer matrix varying the polymer surface charge due to the piezoelectric effect. To test the effect of piezoelectric stimulation on MSCs, our approach is based on a gelatin hydrogel with embedded MSCs and PVDF-CFO electroactive microspheres. Microspheres were produced by electrospray technique, favouring CFO incorporation, crystallisation in beta-phase (85%) and a crystallinity degree of around 55%. The absence of cytotoxicity of the 3D construct was confirmed 24 h after cell encapsulation. Cells were viable, evenly distributed in the hydrogel matrix and surrounded by microspheres, allowing local stimulation. Hydrogels were stimulated using a magnetic bioreactor, and no significant changes were observed in MSCs proliferation in the short or long term. Nevertheless, piezoelectric stimulation upregulated RUNX2 expression after 7 days, indicating the activation of the osteogenic differentiation pathway. These results open the door for optimising a stimulation protocol allowing the application of the magnetically activated 3D electroactive cell culture support for MSCs pre-differentiation before transplantation.This research was funded by Spanish State Research Agency (AEI), G.G.F. acknowledges the support of the PID2019-106000RB-C21/AEI/10.13039/501100011033 project, J.L.G.R. and S.L.-M. acknowledge the support of the PID2019-106099RB-C41 and -C43/AEI/10.13039/501100011033 projects. The CIBER-BBN initiative is funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development. M.G.-F. received government funding for her doctoral thesis, grant number BES-2017-080398. The authors thank FCT (Fundacao para a Ciencia e a Tecnologia) for financial support under the framework of Strategic Funding grant number UID/FIS/04650/2021, projects PTDC/FIS-MAC/28157/2017 and POCI-01-0145-FEDER-007688, and contract under the Stimulus of Scientific Employment, Individual Support 2020.04028 CEECIND (C.M.C.).MDPIDepartamento de Termodinámica AplicadaCentro de Biomateriales e Ingeniería TisularEscuela Técnica Superior de Ingeniería IndustrialAGENCIA ESTATAL DE INVESTIGACIONAgencia Estatal de InvestigaciónEuropean Regional Development FundFundação para a Ciência e a Tecnologia, PortugalRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/193207reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106000RB-C21 HIDROGELES BIOMIMETICOS IMPRIMIBLES CON PRESENTACION DE FACTORES DE CRECIMIENTO EFICIENTE PARA ESTUDIOS DE HEPATOTOXICIDAD DE ALTO RENDIMIENTOAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 AYUDA PARA CONTRATOS PREDOCTORALES PARA LA FORMACIÓN DE DOCTORES 2017 BES-2017-080398 BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACIÓN CELULAR EN INTERFACES CELULA-MATERIAL ELÉCTRICAMENTE ACTIVASAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C41 MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLEFundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 CEEC 2020 2020.04028.CEECINDAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2019-106099RB-C43 DESARROLLO DE ANDAMIAJES BIOMIMETICOS ACTIVOS PARA EL ESTUDIO DE MICROENTORNO DE TUMOR EN OSTEOSARCOMAFundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 Financiamento Estratégico de Unidades de I&D UID%2FFIS%2F04650%2F2021Fundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 Proyectos I&D FCT – PTDC PTDC%2FFIS-MAC%2F28157%2F2017Fundação para a Ciência e a Tecnologia, Portugal https://doi.org/10.13039/501100001871 COMPETE 2020 POCI (FEDER) POCI-01-0145-FEDER-007688open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1932072026-06-13T07:49:27Z
score 15,301629

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