Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum

Pines produce and accumulate oleoresin, which is part of the preformed defensive system or is synthesized de novo in response to biotic and abiotic challenges. Fusarium circinatum is a fungal pathogen that causes Pine Pitch Canker disease and is characterized by cankers with abundant resin at the in...

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Autores: Fariña-Flores, David, Fadón Alberca, A., Hernandez-Escribano, Laura, Fernández De Simón, María Brígida, Conde-García, María, Morales Clemente, M. Teresa, Raposo Llobet, María Rosa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/394975
Acceso en línea:http://hdl.handle.net/10261/394975
https://api.elsevier.com/content/abstract/scopus_id/85211434790
Access Level:acceso abierto
Palabra clave:Constitutive resin
Fungal growth
Fusarium circinatum
Induced resin
Pinus
Pitch canker disease
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spelling Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatumFariña-Flores, DavidFadón Alberca, A.Hernandez-Escribano, LauraFernández De Simón, María BrígidaConde-García, MaríaMorales Clemente, M. TeresaRaposo Llobet, María RosaConstitutive resinFungal growthFusarium circinatumInduced resinPinusPitch canker diseasePines produce and accumulate oleoresin, which is part of the preformed defensive system or is synthesized de novo in response to biotic and abiotic challenges. Fusarium circinatum is a fungal pathogen that causes Pine Pitch Canker disease and is characterized by cankers with abundant resin at the infection site. F. circinatum colonizes the plant using both constitutive and traumatic resin ducts, indicating a resin tolerance that needs to be evaluated. Using a spectrophotometric technique, we assessed the effects of constitutive and induced resin on the growth of F. circinatum. The pathogen was grown in minimum medium supplemented with resin (at 0.8, 1.5 and 3 % concentrations) in the wells of a microplate for 6 days, and the absorbance at 570 nm was measured as an indicator of fungal biomass. The results showed that resin from Pinus pinaster and Pinus radiata enhanced fungal growth, as the absorbances measured with the addition of volatile or nonvolatile fractions extracted from constitutive and induced resins increased above 100 % relative to the solvent used in resin extraction. F. circinatum grew 40 % more in the hexane extract of the induced resin than in the constitutive resin of P. radiata. Terpene profiles determined by GC-MS analysis differed quantitatively and qualitatively by resin type (constitutive or induced) and pine species. The F. circinatum growth with various single terpene components of resin at different concentrations was also measured. The absorbance results showed that α-pinene and citronellol (monoterpenes) promoted growth at specific concentrations, while all others inhibited or did not affect it.This work was supported by MCIN/AEI/10.13039/501100011033 (reference project PID 2020-118734RR-C21). David Fariña-Flores was supported by a fellowship from INIA (PRE 2018-086768), and Laura Hernandez-Escribano was supported by project PID 2020-118734RR-C21.Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Fadón Alberca, A. [0009-0003-3258-1102]Fernández De Simón, María Brígida [0000-0002-2731-4128]Raposo Llobet, María Rosa [0000-0002-4893-6874]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/394975https://api.elsevier.com/content/abstract/scopus_id/85211434790reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118734RR-C21The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.funbio.2024.101525https://doi.org/10.1016/j.funbio.2024.101525Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3949752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
title Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
spellingShingle Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
Fariña-Flores, David
Constitutive resin
Fungal growth
Fusarium circinatum
Induced resin
Pinus
Pitch canker disease
title_short Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
title_full Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
title_fullStr Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
title_full_unstemmed Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
title_sort Effect of Pinus pinaster and Pinus radiata resin on biomass growth of Fusarium circinatum
dc.creator.none.fl_str_mv Fariña-Flores, David
Fadón Alberca, A.
Hernandez-Escribano, Laura
Fernández De Simón, María Brígida
Conde-García, María
Morales Clemente, M. Teresa
Raposo Llobet, María Rosa
author Fariña-Flores, David
author_facet Fariña-Flores, David
Fadón Alberca, A.
Hernandez-Escribano, Laura
Fernández De Simón, María Brígida
Conde-García, María
Morales Clemente, M. Teresa
Raposo Llobet, María Rosa
author_role author
author2 Fadón Alberca, A.
Hernandez-Escribano, Laura
Fernández De Simón, María Brígida
Conde-García, María
Morales Clemente, M. Teresa
Raposo Llobet, María Rosa
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Fadón Alberca, A. [0009-0003-3258-1102]
Fernández De Simón, María Brígida [0000-0002-2731-4128]
Raposo Llobet, María Rosa [0000-0002-4893-6874]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Constitutive resin
Fungal growth
Fusarium circinatum
Induced resin
Pinus
Pitch canker disease
topic Constitutive resin
Fungal growth
Fusarium circinatum
Induced resin
Pinus
Pitch canker disease
description Pines produce and accumulate oleoresin, which is part of the preformed defensive system or is synthesized de novo in response to biotic and abiotic challenges. Fusarium circinatum is a fungal pathogen that causes Pine Pitch Canker disease and is characterized by cankers with abundant resin at the infection site. F. circinatum colonizes the plant using both constitutive and traumatic resin ducts, indicating a resin tolerance that needs to be evaluated. Using a spectrophotometric technique, we assessed the effects of constitutive and induced resin on the growth of F. circinatum. The pathogen was grown in minimum medium supplemented with resin (at 0.8, 1.5 and 3 % concentrations) in the wells of a microplate for 6 days, and the absorbance at 570 nm was measured as an indicator of fungal biomass. The results showed that resin from Pinus pinaster and Pinus radiata enhanced fungal growth, as the absorbances measured with the addition of volatile or nonvolatile fractions extracted from constitutive and induced resins increased above 100 % relative to the solvent used in resin extraction. F. circinatum grew 40 % more in the hexane extract of the induced resin than in the constitutive resin of P. radiata. Terpene profiles determined by GC-MS analysis differed quantitatively and qualitatively by resin type (constitutive or induced) and pine species. The F. circinatum growth with various single terpene components of resin at different concentrations was also measured. The absorbance results showed that α-pinene and citronellol (monoterpenes) promoted growth at specific concentrations, while all others inhibited or did not affect it.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/394975
https://api.elsevier.com/content/abstract/scopus_id/85211434790
url http://hdl.handle.net/10261/394975
https://api.elsevier.com/content/abstract/scopus_id/85211434790
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118734RR-C21
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.funbio.2024.101525
https://doi.org/10.1016/j.funbio.2024.101525
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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