Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks
Direct ink writing (DIW) techniques open up new possibilities for the fabrication of patient-specific bone grafts. Self-setting calcium phosphate inks, which harden at low temperature, allow obtaining nanostructured scaffolds with biomimetic properties and enhanced bioactivity. However, the slow har...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/122135 |
| Acceso en línea: | https://hdl.handle.net/2117/122135 https://dx.doi.org/10.1016/j.actbio.2018.05.042 |
| Access Level: | acceso abierto |
| Palabra clave: | Calcium phosphate Bone regeneration Tissue engineering Hydroxyapatite Biomimetic Bone regeneration 3D plotting Direct ink writing Bone graft Fosfat de calci Ossos -- Regeneració Enginyeria de teixits Hidroxiapatita Àrees temàtiques de la UPC::Enginyeria dels materials |
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Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inksRaymond Llorens, Santiago|||0000-0001-8302-3002Maazouz, YassineMontufar Jiménez, Edgar BenjaminPerez, Roman A.González Arcos, BorjaKonka, Joanna Magdalena|||0000-0001-6593-8532Ginebra Molins, Maria Pau|||0000-0002-4700-5621Calcium phosphateBone regenerationTissue engineeringHydroxyapatiteCalcium phosphateHydroxyapatiteBiomimetic Bone regeneration3D plottingDirect ink writingBone graftFosfat de calciOssos -- RegeneracióEnginyeria de teixitsHidroxiapatitaÀrees temàtiques de la UPC::Enginyeria dels materialsDirect ink writing (DIW) techniques open up new possibilities for the fabrication of patient-specific bone grafts. Self-setting calcium phosphate inks, which harden at low temperature, allow obtaining nanostructured scaffolds with biomimetic properties and enhanced bioactivity. However, the slow hardening kinetics hampers the translation to the clinics. Different hydrothermal treatments for the consolidation of DIW scaffolds fabricated with an a-tricalcium phosphate /pluronic F127 ink were explored, comparing them with a biomimetic treatment. Three different scaffold architectures were analysed. The hardening process, associated to the conversion of a-tricalcium phosphate to hydroxyapatite was drastically accelerated by the hydrothermal treatments, reducing the time for complete reaction from 7¿days to 30 minutes, while preserving the scaffold architectural integrity and retaining the nanostructured features. ß-tricalcium phosphate was formed as a secondary phase, and a change of morphology from plate-like to needle-like crystals in the hydroxyapatite phase was observed. The binder was largely released during the treatment. The hydrothermal treatment resulted in a 30% reduction of the compressive strength, associated to the residual presence of ß-tricalcium phosphate. Biomimetic and hydrothermally treated scaffolds supported the adhesion and proliferation of rat mesenchymal stem cells, indicating a good suitability for bone tissue engineering applications. Statement of Significance 3D plotting has opened up new perspectives in the bone regeneration field allowing the customisation of synthetic bone grafts able to fit patient-specific bone defects. Moreover, this technique allows the control of the scaffolds’ architecture and porosity. The present work introduces a new method to harden biomimetic hydroxyapatite 3D-plotted scaffolds which avoids high-temperature sintering. It has two main advantages: i) it is fast and simple, reducing the whole fabrication process from the several days required for the biomimetic processing to a few hours; and ii) it retains the nanostructured character of biomimetic hydroxyapatite and allows controlling the porosity from the nano- to the macroscale. Moreover, the good in vitro cytocompatibility results support its suitability for cell-based bone regeneration therapiesPeer ReviewedElsevier20182018-01-0120182018-10-10journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/122135https://dx.doi.org/10.1016/j.actbio.2018.05.042reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1221352026-05-27T15:37:01Z |
| dc.title.none.fl_str_mv |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks |
| title |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks |
| spellingShingle |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks Raymond Llorens, Santiago|||0000-0001-8302-3002 Calcium phosphate Bone regeneration Tissue engineering Hydroxyapatite Calcium phosphate Hydroxyapatite Biomimetic Bone regeneration 3D plotting Direct ink writing Bone graft Fosfat de calci Ossos -- Regeneració Enginyeria de teixits Hidroxiapatita Àrees temàtiques de la UPC::Enginyeria dels materials |
| title_short |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks |
| title_full |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks |
| title_fullStr |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks |
| title_full_unstemmed |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks |
| title_sort |
Accelerated hardening of nanotextured 3D-plotted self-setting calcium phosphate inks |
| dc.creator.none.fl_str_mv |
Raymond Llorens, Santiago|||0000-0001-8302-3002 Maazouz, Yassine Montufar Jiménez, Edgar Benjamin Perez, Roman A. González Arcos, Borja Konka, Joanna Magdalena|||0000-0001-6593-8532 Ginebra Molins, Maria Pau|||0000-0002-4700-5621 |
| author |
Raymond Llorens, Santiago|||0000-0001-8302-3002 |
| author_facet |
Raymond Llorens, Santiago|||0000-0001-8302-3002 Maazouz, Yassine Montufar Jiménez, Edgar Benjamin Perez, Roman A. González Arcos, Borja Konka, Joanna Magdalena|||0000-0001-6593-8532 Ginebra Molins, Maria Pau|||0000-0002-4700-5621 |
| author_role |
author |
| author2 |
Maazouz, Yassine Montufar Jiménez, Edgar Benjamin Perez, Roman A. González Arcos, Borja Konka, Joanna Magdalena|||0000-0001-6593-8532 Ginebra Molins, Maria Pau|||0000-0002-4700-5621 |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Calcium phosphate Bone regeneration Tissue engineering Hydroxyapatite Calcium phosphate Hydroxyapatite Biomimetic Bone regeneration 3D plotting Direct ink writing Bone graft Fosfat de calci Ossos -- Regeneració Enginyeria de teixits Hidroxiapatita Àrees temàtiques de la UPC::Enginyeria dels materials |
| topic |
Calcium phosphate Bone regeneration Tissue engineering Hydroxyapatite Calcium phosphate Hydroxyapatite Biomimetic Bone regeneration 3D plotting Direct ink writing Bone graft Fosfat de calci Ossos -- Regeneració Enginyeria de teixits Hidroxiapatita Àrees temàtiques de la UPC::Enginyeria dels materials |
| description |
Direct ink writing (DIW) techniques open up new possibilities for the fabrication of patient-specific bone grafts. Self-setting calcium phosphate inks, which harden at low temperature, allow obtaining nanostructured scaffolds with biomimetic properties and enhanced bioactivity. However, the slow hardening kinetics hampers the translation to the clinics. Different hydrothermal treatments for the consolidation of DIW scaffolds fabricated with an a-tricalcium phosphate /pluronic F127 ink were explored, comparing them with a biomimetic treatment. Three different scaffold architectures were analysed. The hardening process, associated to the conversion of a-tricalcium phosphate to hydroxyapatite was drastically accelerated by the hydrothermal treatments, reducing the time for complete reaction from 7¿days to 30 minutes, while preserving the scaffold architectural integrity and retaining the nanostructured features. ß-tricalcium phosphate was formed as a secondary phase, and a change of morphology from plate-like to needle-like crystals in the hydroxyapatite phase was observed. The binder was largely released during the treatment. The hydrothermal treatment resulted in a 30% reduction of the compressive strength, associated to the residual presence of ß-tricalcium phosphate. Biomimetic and hydrothermally treated scaffolds supported the adhesion and proliferation of rat mesenchymal stem cells, indicating a good suitability for bone tissue engineering applications. Statement of Significance 3D plotting has opened up new perspectives in the bone regeneration field allowing the customisation of synthetic bone grafts able to fit patient-specific bone defects. Moreover, this technique allows the control of the scaffolds’ architecture and porosity. The present work introduces a new method to harden biomimetic hydroxyapatite 3D-plotted scaffolds which avoids high-temperature sintering. It has two main advantages: i) it is fast and simple, reducing the whole fabrication process from the several days required for the biomimetic processing to a few hours; and ii) it retains the nanostructured character of biomimetic hydroxyapatite and allows controlling the porosity from the nano- to the macroscale. Moreover, the good in vitro cytocompatibility results support its suitability for cell-based bone regeneration therapies |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-01-01 2018 2018-10-10 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2117/122135 https://dx.doi.org/10.1016/j.actbio.2018.05.042 |
| url |
https://hdl.handle.net/2117/122135 https://dx.doi.org/10.1016/j.actbio.2018.05.042 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:UPCommons. Portal del coneixement obert de la UPC instname:Universitat Politècnica de Catalunya (UPC) |
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Universitat Politècnica de Catalunya (UPC) |
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UPCommons. Portal del coneixement obert de la UPC |
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UPCommons. Portal del coneixement obert de la UPC |
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15,301603 |