Búsqueda de posibles reservorios de Southern tomato virus (STV) en especies arvenses y otros cultivos hortícolas distintos del tomate
Southern tomato virus (STV) is a virus whose genome consists of a 3.5 Kb double stranded RNA molecule. This virus is phylogenetically related to the Totiviridae (contains nematode and protozoan viruses) and Partitiviridae (containing fungal and plant viruses). It is part of a group of viruses that c...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/89905 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/89905 |
| Access Level: | acceso abierto |
| Palabra clave: | Detection Weeds Tomato Cryptovirus Virus Tomate Especies arvenses Detección PRODUCCION VEGETAL Máster Universitario en Sanidad y Producción Vegetal-Màster Universitari en Sanitat i Producció Vegetal |
| Sumario: | Southern tomato virus (STV) is a virus whose genome consists of a 3.5 Kb double stranded RNA molecule. This virus is phylogenetically related to the Totiviridae (contains nematode and protozoan viruses) and Partitiviridae (containing fungal and plant viruses). It is part of a group of viruses that constitute the new genus Almalgavirus (family Amalgaviridae) cataloged as a group of cryptovirus. STV is not transmitted mechanically or by grafting, but by seed, with a very high efficiency that can reach 90% or more. Since its first detection in Spain in tomato crops in 2013, it has been detected in many tomato plantations of commercial and local varieties of different producing areas. The pathogenic role of this virus is not yet well defined, as it has been frequently detected in asymptomatic plants and in mixed infections with other tomato viruses in symptomatic plants. The aim of this work is to analyze different weeds sampled in tomato fields with STV of the island of Gran Canaria to determine if they can be hosts of this virus and thus be able to act as reservoir of the same. For the development of the assay, ribonucleic acid (RNA) will be obtained from each sampled plant to be analyzed by molecular technique RT-PCR with STV-specific primers. In addition, seedlings of other horticultural crops, with the same molecular technique, will also be analyzed in this study to determine if STV is present in other crops besides tomato. |
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