Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry

[EN]BACKGROUND: Helminth infections in animals to be consumed by humans are an important medical and public health problem. Pharmaceutical research has focused on developing new anthelmintic drugs for parasite control in these animals. However, the incorrect use of anthelmintics can leave residues i...

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Autores: Bustamante Rángel, Myriam, Delgado Zamarreño, María Milagros, Rodríguez Gonzalo, Encarnación
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/155688
Acceso en línea:http://hdl.handle.net/10366/155688
Access Level:acceso abierto
Palabra clave:Anthelmintic drugs
Benzimidazoles
Milk
QuEChERS
LC–MS/MS
2301 Química Analítica
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spelling Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometryBustamante Rángel, MyriamDelgado Zamarreño, María MilagrosRodríguez Gonzalo, EncarnaciónAnthelmintic drugsBenzimidazolesMilkQuEChERSLC–MS/MS2301 Química Analítica[EN]BACKGROUND: Helminth infections in animals to be consumed by humans are an important medical and public health problem. Pharmaceutical research has focused on developing new anthelmintic drugs for parasite control in these animals. However, the incorrect use of anthelmintics can leave residues in animal products intended for human consumption. Their determination is therefore crucial in terms of food safety. RESULTS: In this work, a simple and sensitive method has been developed for the analysis of anthelmintic drugs in milk. The method involves extraction of the analytes using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) method, and separation and determination by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The use of a core-shell column significantly reduced the analysis time compared with conventional columns. The method was validated and applied to the analysis of different commercial milk samples: whole, semi-skimmed and skimmed cows' milk, and goats' milk. None of the benzimidazoles studied was found in the samples analyzed, so these were spiked with the analytes at three concentration levels (10, 50, and 100 ∼g kg−1). CONCLUSIONS: The proposed method provided high sensitivity compared with other methods for the determination of anthelmintics in milk samples, at concentration levels well below the established maximum residue limit (MRLs) values. The proposed method is simple, easy, precise, accurate, and leads to good recovery levels. It can be used successfully for the routine analysisWiley202420242022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/155688reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésSA016G19Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1556882026-06-07T06:28:51Z
dc.title.none.fl_str_mv Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
title Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
spellingShingle Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
Bustamante Rángel, Myriam
Anthelmintic drugs
Benzimidazoles
Milk
QuEChERS
LC–MS/MS
2301 Química Analítica
title_short Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
title_full Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
title_fullStr Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
title_full_unstemmed Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
title_sort Simple method for the determination of anthelmintic drugs in milk intended for human consumption using liquid chromatography–tandem mass spectrometry
dc.creator.none.fl_str_mv Bustamante Rángel, Myriam
Delgado Zamarreño, María Milagros
Rodríguez Gonzalo, Encarnación
author Bustamante Rángel, Myriam
author_facet Bustamante Rángel, Myriam
Delgado Zamarreño, María Milagros
Rodríguez Gonzalo, Encarnación
author_role author
author2 Delgado Zamarreño, María Milagros
Rodríguez Gonzalo, Encarnación
author2_role author
author
dc.subject.none.fl_str_mv Anthelmintic drugs
Benzimidazoles
Milk
QuEChERS
LC–MS/MS
2301 Química Analítica
topic Anthelmintic drugs
Benzimidazoles
Milk
QuEChERS
LC–MS/MS
2301 Química Analítica
description [EN]BACKGROUND: Helminth infections in animals to be consumed by humans are an important medical and public health problem. Pharmaceutical research has focused on developing new anthelmintic drugs for parasite control in these animals. However, the incorrect use of anthelmintics can leave residues in animal products intended for human consumption. Their determination is therefore crucial in terms of food safety. RESULTS: In this work, a simple and sensitive method has been developed for the analysis of anthelmintic drugs in milk. The method involves extraction of the analytes using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) method, and separation and determination by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The use of a core-shell column significantly reduced the analysis time compared with conventional columns. The method was validated and applied to the analysis of different commercial milk samples: whole, semi-skimmed and skimmed cows' milk, and goats' milk. None of the benzimidazoles studied was found in the samples analyzed, so these were spiked with the analytes at three concentration levels (10, 50, and 100 ∼g kg−1). CONCLUSIONS: The proposed method provided high sensitivity compared with other methods for the determination of anthelmintics in milk samples, at concentration levels well below the established maximum residue limit (MRLs) values. The proposed method is simple, easy, precise, accurate, and leads to good recovery levels. It can be used successfully for the routine analysis
publishDate 2022
dc.date.none.fl_str_mv 2022
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/155688
url http://hdl.handle.net/10366/155688
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv SA016G19
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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