Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments

Luminescent nanoparticles are innovative tools for medicine, allowing the imaging of cells and tissues, and, at the same time, carrying and releasing different types of molecules. We explored and compared the loading/release ability of diclofenac (COX-2 antagonist), in both undoped-and luminescent T...

Full description

Bibliographic Details
Authors: Cano Plá, Sandra María, D’Urso, Annarita, Fernández-Sánchez, Jorge F., Colangelo, Donato, Choquesillo-Lazarte, Duane, Ferracini, Ricardo, Bosetti, Michela, Prat, Maria, Gómez-Morales, Jaime
Format: article
Status:Published version
Publication Date:2022
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/334094
Online Access:http://hdl.handle.net/10261/334094
Access Level:Open access
Keyword:Inflammation treatment
Diclofenac-loaded nanoparticles
apatite
Tb3+-doped apatite
Luminescence
Cytocompatibility
Apatite
id ES_8e762cae79bf0b9e62fc0a91e70b4ea2
oai_identifier_str oai:digital.csic.es:10261/334094
network_acronym_str ES
network_name_str España
repository_id_str
spelling Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory EnvironmentsCano Plá, Sandra MaríaD’Urso, AnnaritaFernández-Sánchez, Jorge F.Colangelo, DonatoChoquesillo-Lazarte, DuaneFerracini, RicardoBosetti, MichelaPrat, MariaGómez-Morales, JaimeInflammation treatmentDiclofenac-loaded nanoparticlesapatiteTb3+-doped apatiteLuminescenceCytocompatibilityApatiteLuminescent nanoparticles are innovative tools for medicine, allowing the imaging of cells and tissues, and, at the same time, carrying and releasing different types of molecules. We explored and compared the loading/release ability of diclofenac (COX-2 antagonist), in both undoped-and luminescent Terbium (Tb)-doped citrate-coated carbonated apatite nanoparticles at different temperatures (25, 37, 40 °C) and pHs (7.4, 5.2). The cytocompatibility was evaluated on two osteosarcoma cell lines and primary human osteoblasts. Biological effects of diclofenac-loadednanoparticles were monitored in an in vitro osteoblast’s cytokine–induced inflammation model by evaluating COX-2 mRNA expression and production of PGE2. Adsorption isotherms fitted the multilayer Langmuir-Freundlich model. The maximum adsorbed amounts at 37 °C were higher than at 25 °C, and particularly when using the Tb-doped particles. Diclofenac-release efficiencies were higher at pH 5.2, a condition simulating a local inflammation. The luminescence properties of diclofenac-loaded Tb-doped particles were affected by pH, being the relative luminescence intensity higher at pH 5.2 and the luminescence lifetime higher at pH 7.4, but not influenced either by the temperature or by the diclofenac-loaded amount. Both undoped and Tb-doped nanoparticles were cytocompatible. In addition, diclofenac release increased COX-2 expression and decreased PGE2 production in an in vitro inflammation model. These findings evidence the potential of these nanoparticles for osteo-localized delivery of anti-inflammatory drugs and the possibility to localize the inflammation, characterized by a decrease in pH, by changes in luminescence.We thank Universidad Jorge Tadeo Lozano (Utadeo), National Natural Parks, and the Corales de Profundidad Natural National Park (CPNNP) for their funding and support. The results are products of UTADEO’s projects Ecología y biología del pez león en dos ambientes contrastantes del Caribe colombiano. Fase V (código 822-15-17) and Invasiones biológicas en ambientes tropicales marinos: el caso del pez león (código 901-17-18), and they are within the framework of the subprogram Handling of invading species in the CPNNP. We would like to thank Deibis Seguro from Scuba Cartagena and the Diving Division and Rescue of the National Navy for their support in the capture field trips. Observations with ROV and drifting cameras were made within the projects PRY-BEM-012-14 and Mapeo de la distribución del ensamblaje de Madracis spp., which were funded by Invemar, ANH, and National Parks. We would like to thank Gabriel Navas Suárez and Adriana Bermúdez Tobón for their support in the laboratories of Universidad de Cartagena. Christian Sánchez Díaz prepared the cartography. We would also like to thank the undergraduate and graduate students that cooperated with sample processing, especially Nireth Sierra Sabalza, Shirly Bello Escobar, María Castellanos Jiménez, Margui Almario García, María Rodríguez, and Zuleima Vides Pedrozo. ASM received funding from MincienciasMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320222023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/334094reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/nano12030562Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3340942026-05-22T06:33:51Z
dc.title.none.fl_str_mv Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
title Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
spellingShingle Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
Cano Plá, Sandra María
Inflammation treatment
Diclofenac-loaded nanoparticles
apatite
Tb3+-doped apatite
Luminescence
Cytocompatibility
Apatite
title_short Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
title_full Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
title_fullStr Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
title_full_unstemmed Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
title_sort Biomimetic Citrate-Coated Luminescent Apatite Nanoplatforms for Diclofenac Delivery in Inflammatory Environments
dc.creator.none.fl_str_mv Cano Plá, Sandra María
D’Urso, Annarita
Fernández-Sánchez, Jorge F.
Colangelo, Donato
Choquesillo-Lazarte, Duane
Ferracini, Ricardo
Bosetti, Michela
Prat, Maria
Gómez-Morales, Jaime
author Cano Plá, Sandra María
author_facet Cano Plá, Sandra María
D’Urso, Annarita
Fernández-Sánchez, Jorge F.
Colangelo, Donato
Choquesillo-Lazarte, Duane
Ferracini, Ricardo
Bosetti, Michela
Prat, Maria
Gómez-Morales, Jaime
author_role author
author2 D’Urso, Annarita
Fernández-Sánchez, Jorge F.
Colangelo, Donato
Choquesillo-Lazarte, Duane
Ferracini, Ricardo
Bosetti, Michela
Prat, Maria
Gómez-Morales, Jaime
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Inflammation treatment
Diclofenac-loaded nanoparticles
apatite
Tb3+-doped apatite
Luminescence
Cytocompatibility
Apatite
topic Inflammation treatment
Diclofenac-loaded nanoparticles
apatite
Tb3+-doped apatite
Luminescence
Cytocompatibility
Apatite
description Luminescent nanoparticles are innovative tools for medicine, allowing the imaging of cells and tissues, and, at the same time, carrying and releasing different types of molecules. We explored and compared the loading/release ability of diclofenac (COX-2 antagonist), in both undoped-and luminescent Terbium (Tb)-doped citrate-coated carbonated apatite nanoparticles at different temperatures (25, 37, 40 °C) and pHs (7.4, 5.2). The cytocompatibility was evaluated on two osteosarcoma cell lines and primary human osteoblasts. Biological effects of diclofenac-loadednanoparticles were monitored in an in vitro osteoblast’s cytokine–induced inflammation model by evaluating COX-2 mRNA expression and production of PGE2. Adsorption isotherms fitted the multilayer Langmuir-Freundlich model. The maximum adsorbed amounts at 37 °C were higher than at 25 °C, and particularly when using the Tb-doped particles. Diclofenac-release efficiencies were higher at pH 5.2, a condition simulating a local inflammation. The luminescence properties of diclofenac-loaded Tb-doped particles were affected by pH, being the relative luminescence intensity higher at pH 5.2 and the luminescence lifetime higher at pH 7.4, but not influenced either by the temperature or by the diclofenac-loaded amount. Both undoped and Tb-doped nanoparticles were cytocompatible. In addition, diclofenac release increased COX-2 expression and decreased PGE2 production in an in vitro inflammation model. These findings evidence the potential of these nanoparticles for osteo-localized delivery of anti-inflammatory drugs and the possibility to localize the inflammation, characterized by a decrease in pH, by changes in luminescence.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/334094
url http://hdl.handle.net/10261/334094
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/nano12030562
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413130843455488
score 15,811543

Similar Items