R‐loops cause replication impairment and genome instability during meiosis

R‐loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R‐loops in meiosis. We found that both S....

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Detalhes bibliográficos
Autores: Castellano Pozo, Maikel, García Muse, Tatiana, Aguilera López, Andrés
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/167049
Acesso em linha:https://hdl.handle.net/11441/167049
https://doi.org/10.1038/embor.2012.119
Access Level:acceso abierto
Palavra-chave:Caenorhabditis elegans
genome instability
meiotic replication
R-loops
Saccharomyces cerevisiae
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spelling R‐loops cause replication impairment and genome instability during meiosisCastellano Pozo, MaikelGarcía Muse, TatianaAguilera López, AndrésCaenorhabditis elegansgenome instabilitymeiotic replicationR-loopsSaccharomyces cerevisiaeR‐loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R‐loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA‐damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA‐damage accumulation. We conclude that R‐loops can form during meiosis causing replication impairment with deleterious results.Ministry of Science and Innovation (BFU2006‐05260, BFU2010‐16372 and Consolider Ingenio 2010 CSD2007‐0015), the European Union (FEDER) and the Junta de Andalucía (BIO102 and CVI4567).EMBO PressGenéticaMinisterio de Ciencia e Innovación (MICIN). EspañaEuropean Union (UE)Junta de Andalucía2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/167049https://doi.org/10.1038/embor.2012.119reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBFU2006‐05260BFU2010‐163722010 CSD2007‐0015BIO102CVI4567https://dx.doi.org/10.1038/embor.2012.119info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1670492026-06-17T12:51:07Z
dc.title.none.fl_str_mv R‐loops cause replication impairment and genome instability during meiosis
title R‐loops cause replication impairment and genome instability during meiosis
spellingShingle R‐loops cause replication impairment and genome instability during meiosis
Castellano Pozo, Maikel
Caenorhabditis elegans
genome instability
meiotic replication
R-loops
Saccharomyces cerevisiae
title_short R‐loops cause replication impairment and genome instability during meiosis
title_full R‐loops cause replication impairment and genome instability during meiosis
title_fullStr R‐loops cause replication impairment and genome instability during meiosis
title_full_unstemmed R‐loops cause replication impairment and genome instability during meiosis
title_sort R‐loops cause replication impairment and genome instability during meiosis
dc.creator.none.fl_str_mv Castellano Pozo, Maikel
García Muse, Tatiana
Aguilera López, Andrés
author Castellano Pozo, Maikel
author_facet Castellano Pozo, Maikel
García Muse, Tatiana
Aguilera López, Andrés
author_role author
author2 García Muse, Tatiana
Aguilera López, Andrés
author2_role author
author
dc.contributor.none.fl_str_mv Genética
Ministerio de Ciencia e Innovación (MICIN). España
European Union (UE)
Junta de Andalucía
dc.subject.none.fl_str_mv Caenorhabditis elegans
genome instability
meiotic replication
R-loops
Saccharomyces cerevisiae
topic Caenorhabditis elegans
genome instability
meiotic replication
R-loops
Saccharomyces cerevisiae
description R‐loops are harmful structures with a negative impact on transcription and recombination during mitosis, but no information exists for meiosis. We used Saccharomyces cerevisiae and Caenorhabditis elegans THO mutants as a tool to determine the consequences of R‐loops in meiosis. We found that both S. cerevisiae and C. elegans THO mutants show defective meiosis and an impairment of premeiotic replication as well as DNA‐damage accumulation. Importantly, RNase H partially suppressed the replication impairment and the DNA‐damage accumulation. We conclude that R‐loops can form during meiosis causing replication impairment with deleterious results.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/167049
https://doi.org/10.1038/embor.2012.119
url https://hdl.handle.net/11441/167049
https://doi.org/10.1038/embor.2012.119
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BFU2006‐05260
BFU2010‐16372
2010 CSD2007‐0015
BIO102
CVI4567
https://dx.doi.org/10.1038/embor.2012.119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv EMBO Press
publisher.none.fl_str_mv EMBO Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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