Pharmacological characterization of alpha adrenoceptor-mediated motor responses in the rat colon
Background: Inhibitory neuromuscular transmission in the gastrointestinal tract is mediated by intrinsic nitrergic and purinergic neurons. Purines activate G protein-coupled receptor P2Y receptors, increasing intracellular Ca that activates small conductance calcium-activated potassium (SK) channels...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:302960 |
| Acceso en línea: | https://ddd.uab.cat/record/302960 https://dx.doi.org/urn:doi:10.1111/nmo.14921 |
| Access Level: | acceso abierto |
| Palabra clave: | Alpha adrenoceptors Apamin Colon Phenylephrine Relaxation |
| Sumario: | Background: Inhibitory neuromuscular transmission in the gastrointestinal tract is mediated by intrinsic nitrergic and purinergic neurons. Purines activate G protein-coupled receptor P2Y receptors, increasing intracellular Ca that activates small conductance calcium-activated potassium (SK) channels. Little is known about the effect of adrenergic receptor activation on intestinal smooth muscle. In vascular tissue, stimulation of α-adrenoceptors causes smooth muscle contraction, while their effect on intestinal tissue is poorly understood. This study aimed to pharmacologically characterize the effect of α-adrenoceptor activation in the rat colon, which shares similar inhibitory pathways to the human colon. Methods: Muscle bath experiments were performed with the rat proximal, mid, and distal colon oriented both circularly and longitudinally. Results: The α-adrenoceptor agonist phenylephrine (PE) (10-10 M) evoked concentration-dependent relaxations of the intestinal smooth muscle from all regions and orientations. However, in the mid-circular colon at low PE concentrations, a contraction sensitive to 10 M phentolamine (non-selective α-adrenoceptor blocker), the neural blocker tetrodotoxin (TTX; 10 M), and atropine (10 M) was recorded. PE-induced relaxations were insensitive to TTX (10 M) and the nonselective β-adrenoceptor blocker propranolol (10 M). In contrast, PE-induced relaxations were blocked by phentolamine (10 M), prazosin (10 M) (α-adrenoceptor blocker), and RS17053 (10 M) (α-blocker), but not by yohimbine (10 M) (α-adrenoceptor blocker). Apamin (10 M), a SK channel blocker, abolished PE-induced relaxations. Conclusions: Contractile responses in the circular muscle of the mid colon could be attributed to α-adrenoceptors located on enteric cholinergic neurons. Stimulation of α-adrenoreceptors activates SK channels to cause smooth muscle relaxation, which constitutes a signaling pathway that shares similarities with P2Y receptors. |
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