Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions

This work sought to test the positive effect of red LED light on frozen bovine sperm resistance to thawing in suboptimal conditions. Moreover, a preliminary study explores whether this improvement could have any repercussions on in vivo fertilizing ability. Thus, frozen bull semen straws (n = 16) we...

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Autores: Blanco-Prieto, Olga, Maside, Carolina, Gruzmacher, Andrea, Ortiz, Manuel, Ratto, Marcelo, Urra, Francisco Javier, Vera, Tomás, Strobel, Pablo, Catalán, Jaime, Mislei , Beatrice, Bucci, Diego, Yeste, Marc, Rodríguez-Gil, Joan E., Ramírez-Reveco, Alfredo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/469413
Acceso en línea:https://doi.org/10.3390/ani15223353
https://hdl.handle.net/10459.1/469413
Access Level:acceso abierto
Palabra clave:Red light
Irradiation
Cryopreserved sperm
Artificial insemination
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spelling Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal ConditionsBlanco-Prieto, OlgaMaside, CarolinaGruzmacher, AndreaOrtiz, ManuelRatto, MarceloUrra, Francisco JavierVera, TomásStrobel, PabloCatalán, JaimeMislei , BeatriceBucci, DiegoYeste, MarcRodríguez-Gil, Joan E.Ramírez-Reveco, AlfredoRed lightIrradiationCryopreserved spermArtificial inseminationThis work sought to test the positive effect of red LED light on frozen bovine sperm resistance to thawing in suboptimal conditions. Moreover, a preliminary study explores whether this improvement could have any repercussions on in vivo fertilizing ability. Thus, frozen bull semen straws (n = 16) were thawed (a) with irradiation for 5 min at 20 °C (2 min light, 1 min darkness, 2 min light; PHOTO); (b) without irradiation for 5 min at 20 °C (ET); or (c) through immersion into a water bath at 38 °C for 40 s as standard control (CONTROL). Sperm quality and preliminary, purely descriptive AI trials were performed. The PHOTO samples demonstrated significantly (p < 0.05) improved percentages of intact acrosomes, progressive motility, DNA condensation and fragmentation, and viable sperm with high ROS/superoxides. The viability of PHOTO samples decreased significantly (p < 0.05) when compared with the ET ones. Overall results of both the PHOTO and ET samples were poorer than those of the CONTROL. Otherwise, the PHOTO straws yielded greater pregnancy rates (64.0% vs. 49.4% in CONTROL) when evaluated at two different farms. The results suggest that irradiating cryopreserved bovine sperm during thawing in suboptimal conditions could improve AI pregnancy rates, although more in vivo studies are needed to support this conclusion.This research was funded by the Ministry of Science, Technology, Knowledge and Innovation, Chile (PCI-MEC-CONICYT 80150017 and ANID-FOVI210036); the Ministry of Science and Innovation, Spain (AGL2017-88329-R and PID2020-113320RB-I00); the Regional Government of Catalonia, Spain (2017-SGR-1229 and 2021-SGR-00900); and the Catalan Institution for Research and Advanced Studies, Spain (ICREA).MDPI2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/ani15223353https://hdl.handle.net/10459.1/469413reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00Reproducció del document publicat a: https://doi.org/10.3390/ani15223353Animals, 2025, vol. 15, núm 22cc-by (c) Blanco-Prieto, et al., 2025Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4694132026-06-24T12:42:17Z
dc.title.none.fl_str_mv Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
title Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
spellingShingle Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
Blanco-Prieto, Olga
Red light
Irradiation
Cryopreserved sperm
Artificial insemination
title_short Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
title_full Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
title_fullStr Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
title_full_unstemmed Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
title_sort Red LED Light Irradiation Increases the Resistance Against Environmental Stress of Frozen Bovine Sperm Thawed in Suboptimal Conditions
dc.creator.none.fl_str_mv Blanco-Prieto, Olga
Maside, Carolina
Gruzmacher, Andrea
Ortiz, Manuel
Ratto, Marcelo
Urra, Francisco Javier
Vera, Tomás
Strobel, Pablo
Catalán, Jaime
Mislei , Beatrice
Bucci, Diego
Yeste, Marc
Rodríguez-Gil, Joan E.
Ramírez-Reveco, Alfredo
author Blanco-Prieto, Olga
author_facet Blanco-Prieto, Olga
Maside, Carolina
Gruzmacher, Andrea
Ortiz, Manuel
Ratto, Marcelo
Urra, Francisco Javier
Vera, Tomás
Strobel, Pablo
Catalán, Jaime
Mislei , Beatrice
Bucci, Diego
Yeste, Marc
Rodríguez-Gil, Joan E.
Ramírez-Reveco, Alfredo
author_role author
author2 Maside, Carolina
Gruzmacher, Andrea
Ortiz, Manuel
Ratto, Marcelo
Urra, Francisco Javier
Vera, Tomás
Strobel, Pablo
Catalán, Jaime
Mislei , Beatrice
Bucci, Diego
Yeste, Marc
Rodríguez-Gil, Joan E.
Ramírez-Reveco, Alfredo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Red light
Irradiation
Cryopreserved sperm
Artificial insemination
topic Red light
Irradiation
Cryopreserved sperm
Artificial insemination
description This work sought to test the positive effect of red LED light on frozen bovine sperm resistance to thawing in suboptimal conditions. Moreover, a preliminary study explores whether this improvement could have any repercussions on in vivo fertilizing ability. Thus, frozen bull semen straws (n = 16) were thawed (a) with irradiation for 5 min at 20 °C (2 min light, 1 min darkness, 2 min light; PHOTO); (b) without irradiation for 5 min at 20 °C (ET); or (c) through immersion into a water bath at 38 °C for 40 s as standard control (CONTROL). Sperm quality and preliminary, purely descriptive AI trials were performed. The PHOTO samples demonstrated significantly (p < 0.05) improved percentages of intact acrosomes, progressive motility, DNA condensation and fragmentation, and viable sperm with high ROS/superoxides. The viability of PHOTO samples decreased significantly (p < 0.05) when compared with the ET ones. Overall results of both the PHOTO and ET samples were poorer than those of the CONTROL. Otherwise, the PHOTO straws yielded greater pregnancy rates (64.0% vs. 49.4% in CONTROL) when evaluated at two different farms. The results suggest that irradiating cryopreserved bovine sperm during thawing in suboptimal conditions could improve AI pregnancy rates, although more in vivo studies are needed to support this conclusion.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/ani15223353
https://hdl.handle.net/10459.1/469413
url https://doi.org/10.3390/ani15223353
https://hdl.handle.net/10459.1/469413
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00
Reproducció del document publicat a: https://doi.org/10.3390/ani15223353
Animals, 2025, vol. 15, núm 22
dc.rights.none.fl_str_mv cc-by (c) Blanco-Prieto, et al., 2025
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Blanco-Prieto, et al., 2025
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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