Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment

Salmonella Typhi is an intracellular bacterium causing a variety of enteric diseases, being typhoid fever the most common. Current modalities for treating S. typhi infection are subjected to multi-drug resistance. Herein, a novel macrophage targeting approach was developed via coating bioinspired ma...

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Autores: Arshad, Rabia, Salman, Muhammad, Malik, Abdul, Alkholief, Musaed, Tabish, Tanveer A., Moghadam, Ali Abbasi, Rahdar, Abbas, Díez Pascual, Ana María|||0000-0001-7405-2354
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/60866
Acceso en línea:http://hdl.handle.net/10017/60866
https://dx.doi.org/10.1016/j.ijbiomac.2023.124741
Access Level:acceso abierto
Palabra clave:Mannosylated hyaluronic acid
Salmonella Typhi
Drug delivery
Química
Chemistry
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spelling Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatmentArshad, RabiaSalman, MuhammadMalik, AbdulAlkholief, MusaedTabish, Tanveer A.Moghadam, Ali AbbasiRahdar, AbbasDíez Pascual, Ana María|||0000-0001-7405-2354Mannosylated hyaluronic acidSalmonella TyphiDrug deliveryQuímicaChemistrySalmonella Typhi is an intracellular bacterium causing a variety of enteric diseases, being typhoid fever the most common. Current modalities for treating S. typhi infection are subjected to multi-drug resistance. Herein, a novel macrophage targeting approach was developed via coating bioinspired mannosylated preactivated hyaluronic acid (Man-PTHA) ligands on a self-nanoemulsifying drug delivery system (SNEDDS) loaded with the anti-bacterial drug ciprofloxacin (CIP). The shake flask method was used to determine the drug solubility in the different excipients (oil, surfactants and co-surfactants). Man-PTHA were characterized by physicochemical, in vitro, and in vivo parameters. The mean droplet size was 257 nm, with a PDI of 0.37 and zeta potential of ?15 mV. In 72 h, 85 % of the drug was released in a sustained manner, and the entrapment efficiency was 95 %. Outstanding biocompatibility, mucoadhesion, muco-penetration, anti-bacterial action and hemocompatibility were observed. Intra-macrophage survival of S. typhi was minimal (1 %) with maximum nanoparticle uptake, as shown by their higher fluorescence intensity. Serum biochemistry evaluation showed no significant changes or toxicity, and histopathological evaluation confirmed the entero-protective nature of the bioinspired polymers. Overall, results confirm that Man-PTHA SNEDDS can be employed as novel and effective delivery systems for the therapeutic management of S. typhi infection.Comunidad de MadridUniversidad de Alcalá20232023-06-10journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/60866https://dx.doi.org/10.1016/j.ijbiomac.2023.124741reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available EPU-INV%2F2020%2F012open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/608662026-06-18T11:13:07Z
dc.title.none.fl_str_mv Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
title Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
spellingShingle Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
Arshad, Rabia
Mannosylated hyaluronic acid
Salmonella Typhi
Drug delivery
Química
Chemistry
title_short Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
title_full Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
title_fullStr Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
title_full_unstemmed Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
title_sort Mannosylated preactivated hyaluronic acid-based nanostructures for bacterial infection treatment
dc.creator.none.fl_str_mv Arshad, Rabia
Salman, Muhammad
Malik, Abdul
Alkholief, Musaed
Tabish, Tanveer A.
Moghadam, Ali Abbasi
Rahdar, Abbas
Díez Pascual, Ana María|||0000-0001-7405-2354
author Arshad, Rabia
author_facet Arshad, Rabia
Salman, Muhammad
Malik, Abdul
Alkholief, Musaed
Tabish, Tanveer A.
Moghadam, Ali Abbasi
Rahdar, Abbas
Díez Pascual, Ana María|||0000-0001-7405-2354
author_role author
author2 Salman, Muhammad
Malik, Abdul
Alkholief, Musaed
Tabish, Tanveer A.
Moghadam, Ali Abbasi
Rahdar, Abbas
Díez Pascual, Ana María|||0000-0001-7405-2354
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mannosylated hyaluronic acid
Salmonella Typhi
Drug delivery
Química
Chemistry
topic Mannosylated hyaluronic acid
Salmonella Typhi
Drug delivery
Química
Chemistry
description Salmonella Typhi is an intracellular bacterium causing a variety of enteric diseases, being typhoid fever the most common. Current modalities for treating S. typhi infection are subjected to multi-drug resistance. Herein, a novel macrophage targeting approach was developed via coating bioinspired mannosylated preactivated hyaluronic acid (Man-PTHA) ligands on a self-nanoemulsifying drug delivery system (SNEDDS) loaded with the anti-bacterial drug ciprofloxacin (CIP). The shake flask method was used to determine the drug solubility in the different excipients (oil, surfactants and co-surfactants). Man-PTHA were characterized by physicochemical, in vitro, and in vivo parameters. The mean droplet size was 257 nm, with a PDI of 0.37 and zeta potential of ?15 mV. In 72 h, 85 % of the drug was released in a sustained manner, and the entrapment efficiency was 95 %. Outstanding biocompatibility, mucoadhesion, muco-penetration, anti-bacterial action and hemocompatibility were observed. Intra-macrophage survival of S. typhi was minimal (1 %) with maximum nanoparticle uptake, as shown by their higher fluorescence intensity. Serum biochemistry evaluation showed no significant changes or toxicity, and histopathological evaluation confirmed the entero-protective nature of the bioinspired polymers. Overall, results confirm that Man-PTHA SNEDDS can be employed as novel and effective delivery systems for the therapeutic management of S. typhi infection.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-06-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/60866
https://dx.doi.org/10.1016/j.ijbiomac.2023.124741
url http://hdl.handle.net/10017/60866
https://dx.doi.org/10.1016/j.ijbiomac.2023.124741
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available EPU-INV%2F2020%2F012
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
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