Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect an immune complex formed between β-lactoglobulin and polyclonal anti-β-lactoglobulin antibody in the gas phase. The most important experimental parameters to detect such a specific antibody-antigen complex by...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2003 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/89903 |
| Acceso en línea: | http://hdl.handle.net/10261/89903 |
| Access Level: | acceso abierto |
| Sumario: | Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect an immune complex formed between β-lactoglobulin and polyclonal anti-β-lactoglobulin antibody in the gas phase. The most important experimental parameters to detect such a specific antibody-antigen complex by MALDI were the use of solutions at near-neutral pH and of sinapinic acid matrix prepared by the dried-droplet method. Under such conditions, predominantly one but also two molecules of antigen protein were complexed by the antibody. Specific formation of the antibody-antigen complex was confirmed by performing competitive reactions. Addition of antibody to a 1:1 mixture of β-lactoglobulin and one control protein resulted not only in the appearance of the expected antibody-antigen complex, but also in a strong decrease in the free β-lactoglobulin signal, while the abundance of the control protein was not influenced. Copyright © 2003 John Wiley & Sons, Ltd. |
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