Acrosomal status and PARP-1 nuclear markers could improve discrimination of potential fertility in good-quality boar semen doses
[EN] Reproductive efficiency partly depends on boar semen quality. However, it is challenging to predict sperm fertility once acceptable quality endpoints have been met. This study aims to establish a link between different semen quality parameters and farm fertility outcomes in semen doses selected...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/26384 |
| Acceso en línea: | https://onlinelibrary.wiley.com/doi/10.1111/rda.70145 https://hdl.handle.net/10612/26384 |
| Access Level: | acceso abierto |
| Palabra clave: | Producción animal Zoología Boar Semen Semen analysis Seminal plasma Swine reproduction 3104.11 Reproducción |
| Sumario: | [EN] Reproductive efficiency partly depends on boar semen quality. However, it is challenging to predict sperm fertility once acceptable quality endpoints have been met. This study aims to establish a link between different semen quality parameters and farm fertility outcomes in semen doses selected for commercial use. We analysed 105 ejaculates from 15 adult Pietrain boars, extended into 45 mL artificial insemination (AI) doses. A total of 605 sows were inseminated (40.1±7.8 females/ boar), and fertility, farrowing rate, and prolificacy data were recorded. Sperm evaluation included sperm plasma analysis, kinematics, morphology, viability, acrosome integrity, apoptotic-like changes, mitochondrial activity, and DNA damage (DNA fragmentation poly-ADP ribose polymerase 1, PARP1, together with its product, PAR, and its cleaved form cPARP). The sperm membrane and acrosome were evaluated using the short hypoosmotic swelling test (sHOST) and the osmotic resistance test (ORT). Fertility and farrowing rates exceeded 94%, with an average of 20.18±2.03 piglets born/litter (average alive born: 87.75±5.61%). Negative correlations were found between damaged acrosomes and ultrasound fertility (rho=-0.240, p=0.021), farrowing rate (rho=-0.244; p=0.019), and total born (r=-0.304; p=0.003). Average born alive was positively correlated with plasma seminal concentrations of protein (r=0.273; p=0.009), fructose (r=0.243; p=0.024) and cathepsine B (rho=0.257; p=0.029), but negatively correlated with apoptosis and DNA damage nuclear markers, cPARP (r=-0.295, p =0.005) and PAR (r=-0.209, p=0.049). However, regression models only showed significant results for predicting the total born from damaged acrosomes and the alive born from cPARP. Since semen quality and fertility were good, most parameters did not affect fertility outcomes. Acrosomal damage follows previous studies as a reliable predictor of reproductive outcomes, whereas cPARP shows potential as a novel biomarker |
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