Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm

This article belongs to the Special Issue Recent Advancements in the Development of Antiprotozoal Agents.

Detalhes bibliográficos
Autores: Hernández-Torres, Mario Alberto, García-Davis, Sara, Fernández, José J., Díaz-Marrero, Ana Raquel, Hernández-García, Magda Elizabeth, Carranza-Torres, Irma Edith, Viveros-Valdez, Ezequiel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/404716
Acesso em linha:http://hdl.handle.net/10261/404716
Access Level:acceso abierto
Palavra-chave:Tagetes nelsonii
Triterpenes
Betulin
Stigmasterol
Anti-trichomonal activity
Molecular docking
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spelling Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii GreenmHernández-Torres, Mario AlbertoGarcía-Davis, SaraFernández, José J.Díaz-Marrero, Ana RaquelHernández-García, Magda ElizabethCarranza-Torres, Irma EdithViveros-Valdez, EzequielTagetes nelsoniiTriterpenesBetulinStigmasterolAnti-trichomonal activityMolecular dockingThis article belongs to the Special Issue Recent Advancements in the Development of Antiprotozoal Agents.[Background:] Trichomonas vaginalis is the causative agent of human trichomoniasis, the most common non-viral sexually transmitted infection. This disease is associated with an increased susceptibility to HIV and HPV infections. Currently, resistance to metronidazole (MTZ), the main drug used for treatment, has been reported in up to 9.6% of cases; additionally, the compound is also associated with adverse side effects. Therefore, it is urgent to identify new treatment options[Objective:] In this study, we investigated for the first time the in vitro and in silico activity against T. vaginalis of betulin and stigmasterol isolated from Tagetes nelsonii Greenm, as well as their hemolytic activity.[Methods:] Plant specimen was collected in Chiapas, Mexico. Hexane and methanol extracts were prepared through sonication-assisted maceration. The antiprotozoal and hemolytic activities were evaluated in vitro against Trichomonas vaginalis trophozoites and human erythrocytes. The most active extract was fractionated using chromatographic techniques in a bioassay-guided study. The active metabolites were identified by 1H and 13C-NMR spectroscopy, and their biological activity was further assessed in silico against lactate dehydrogenase (LDH), pyruvate ferredoxin oxidoreductase (PFOR) methionine gamma-lyase (MGL) and purine nucleoside phosphorylase (PNP) T. vaginalis enzymes[Results:] Both triterpenes showed anti-trichomonal activity and no hemolytic activity at 100 µg/mL. Molecular docking studies predicted promising interactions of triterpenes with T. vaginalis drug target proteins, TvpFOR and TvLDH.[Conclusions:] Our results revealed that betulin and stigmasterol are potential molecules for the development of new trichomonacidal therapies against T. vaginalis.This research was funded by the Government of the Canary Islands ProID202401003 (BIMICMAR) and PROMOTUR Turismo de Canarias (Actuaciones de Cohesión de Destino—Canarias Ecoínsulas II), and specifically PROMOTUR_SP3: “Islas Canarias, naturaleza marina singular: Salud y bienestar”, co-funded by the European Union NextGenerationEU/PRTR.Peer reviewedMultidisciplinary Digital Publishing InstituteGobierno de CanariasTurismo de Islas CanariasEuropean CommissionFernández, José J. [0000-0002-0805-8317]Díaz-Marrero, Ana Raquel [0000-0002-8886-7519]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/404716reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/ph18101587https://doi.org/10.3390/ph18101587Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4047162026-05-22T06:33:51Z
dc.title.none.fl_str_mv Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
title Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
spellingShingle Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
Hernández-Torres, Mario Alberto
Tagetes nelsonii
Triterpenes
Betulin
Stigmasterol
Anti-trichomonal activity
Molecular docking
title_short Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
title_full Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
title_fullStr Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
title_full_unstemmed Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
title_sort Anti-Trichomonas vaginalis Activity of Triterpenes from Tagetes nelsonii Greenm
dc.creator.none.fl_str_mv Hernández-Torres, Mario Alberto
García-Davis, Sara
Fernández, José J.
Díaz-Marrero, Ana Raquel
Hernández-García, Magda Elizabeth
Carranza-Torres, Irma Edith
Viveros-Valdez, Ezequiel
author Hernández-Torres, Mario Alberto
author_facet Hernández-Torres, Mario Alberto
García-Davis, Sara
Fernández, José J.
Díaz-Marrero, Ana Raquel
Hernández-García, Magda Elizabeth
Carranza-Torres, Irma Edith
Viveros-Valdez, Ezequiel
author_role author
author2 García-Davis, Sara
Fernández, José J.
Díaz-Marrero, Ana Raquel
Hernández-García, Magda Elizabeth
Carranza-Torres, Irma Edith
Viveros-Valdez, Ezequiel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Gobierno de Canarias
Turismo de Islas Canarias
European Commission
Fernández, José J. [0000-0002-0805-8317]
Díaz-Marrero, Ana Raquel [0000-0002-8886-7519]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Tagetes nelsonii
Triterpenes
Betulin
Stigmasterol
Anti-trichomonal activity
Molecular docking
topic Tagetes nelsonii
Triterpenes
Betulin
Stigmasterol
Anti-trichomonal activity
Molecular docking
description This article belongs to the Special Issue Recent Advancements in the Development of Antiprotozoal Agents.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/404716
url http://hdl.handle.net/10261/404716
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/ph18101587
https://doi.org/10.3390/ph18101587
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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