A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase
Glutamate 5-kinase (G5K) makes the highly unstable product glutamyl 5-phosphate (G5P) in the initial, controlling step of proline/ornithine synthesis, being feedback-inhibited by proline or ornithine, and causing, when defective, clinical hyperammonaemia. We determined two crystal structures of G5K...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2007 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/111016 |
| Acceso en línea: | http://hdl.handle.net/10261/111016 |
| Access Level: | acceso abierto |
| Palabra clave: | Δ1-pyrroline 5-carboxylate synthetase PUA domain ornithine synthesis proline synthesis Amino acid kinase family |
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A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinaseMarco-Marín, ClaraGil-Ortiz, FernandoPérez-Arellano, IsabelCervera, JavierFita, IgnacioRubio, VicenteΔ1-pyrroline 5-carboxylate synthetasePUA domainornithine synthesisproline synthesisAmino acid kinase familyGlutamate 5-kinase (G5K) makes the highly unstable product glutamyl 5-phosphate (G5P) in the initial, controlling step of proline/ornithine synthesis, being feedback-inhibited by proline or ornithine, and causing, when defective, clinical hyperammonaemia. We determined two crystal structures of G5K from Escherichia coli, at 2.9 Å and 2.5 Å resolution, complexed with glutamate and sulphate, or with G5P, sulphate and the proline analogue 5-oxoproline. E. coli G5K presents a novel tetrameric (dimer of dimers) architecture. Each subunit contains a 257 residue AAK domain, typical of acylphosphate-forming enzymes, with characteristic α3β8α4 sandwich topology. This domain is responsible for catalysis and proline inhibition, and has a crater on the β sheet C-edge that hosts the active centre and bound 5-oxoproline. Each subunit contains a 93 residue C-terminal PUA domain, typical of RNA-modifying enzymes, which presents the characteristic β5β4 sandwich fold and three α helices. The AAK and PUA domains of one subunit associate non-canonically in the dimer with the same domains of the other subunit, leaving a negatively charged hole between them that hosts two Mg ions in one crystal, in line with the G5K requirement for free Mg. The tetramer, formed by two dimers interacting exclusively through their AAK domains, is flat and elongated, and has in each face, pericentrically, two exposed active centres in alternate subunits. This would permit the close apposition of two active centres of bacterial glutamate-5-phosphate reductase (the next enzyme in the proline/ornithine-synthesising route), supporting the postulated channelling of G5P. The structures clarify substrate binding and catalysis, justify the high glutamate specificity, explain the effects of known point mutations, and support the binding of proline near glutamate. Proline binding may trigger the movement of a loop that encircles glutamate, and which participates in a hydrogen bond network connecting active centres, which is possibly involved in the cooperativity for glutamate. © 2007 Elsevier Ltd. All rights reserved.This work was supported by grants BFU2004-05159, BFU2004-04472 from the Spanish Ministry of Education and Science, and PI052838 from the Spanish Ministry of Health.Peer ReviewedAcademic PressMinisterio de Educación y Ciencia (España)Ministerio de Sanidad y Consumo (España)2015201520072015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/111016reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.jmb.2007.01.073info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1110162026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase |
| title |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase |
| spellingShingle |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase Marco-Marín, Clara Δ1-pyrroline 5-carboxylate synthetase PUA domain ornithine synthesis proline synthesis Amino acid kinase family |
| title_short |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase |
| title_full |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase |
| title_fullStr |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase |
| title_full_unstemmed |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase |
| title_sort |
A Novel Two-domain Architecture Within the Amino Acid Kinase Enzyme Family Revealed by the Crystal Structure of Escherichia coli Glutamate 5-kinase |
| dc.creator.none.fl_str_mv |
Marco-Marín, Clara Gil-Ortiz, Fernando Pérez-Arellano, Isabel Cervera, Javier Fita, Ignacio Rubio, Vicente |
| author |
Marco-Marín, Clara |
| author_facet |
Marco-Marín, Clara Gil-Ortiz, Fernando Pérez-Arellano, Isabel Cervera, Javier Fita, Ignacio Rubio, Vicente |
| author_role |
author |
| author2 |
Gil-Ortiz, Fernando Pérez-Arellano, Isabel Cervera, Javier Fita, Ignacio Rubio, Vicente |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Educación y Ciencia (España) Ministerio de Sanidad y Consumo (España) |
| dc.subject.none.fl_str_mv |
Δ1-pyrroline 5-carboxylate synthetase PUA domain ornithine synthesis proline synthesis Amino acid kinase family |
| topic |
Δ1-pyrroline 5-carboxylate synthetase PUA domain ornithine synthesis proline synthesis Amino acid kinase family |
| description |
Glutamate 5-kinase (G5K) makes the highly unstable product glutamyl 5-phosphate (G5P) in the initial, controlling step of proline/ornithine synthesis, being feedback-inhibited by proline or ornithine, and causing, when defective, clinical hyperammonaemia. We determined two crystal structures of G5K from Escherichia coli, at 2.9 Å and 2.5 Å resolution, complexed with glutamate and sulphate, or with G5P, sulphate and the proline analogue 5-oxoproline. E. coli G5K presents a novel tetrameric (dimer of dimers) architecture. Each subunit contains a 257 residue AAK domain, typical of acylphosphate-forming enzymes, with characteristic α3β8α4 sandwich topology. This domain is responsible for catalysis and proline inhibition, and has a crater on the β sheet C-edge that hosts the active centre and bound 5-oxoproline. Each subunit contains a 93 residue C-terminal PUA domain, typical of RNA-modifying enzymes, which presents the characteristic β5β4 sandwich fold and three α helices. The AAK and PUA domains of one subunit associate non-canonically in the dimer with the same domains of the other subunit, leaving a negatively charged hole between them that hosts two Mg ions in one crystal, in line with the G5K requirement for free Mg. The tetramer, formed by two dimers interacting exclusively through their AAK domains, is flat and elongated, and has in each face, pericentrically, two exposed active centres in alternate subunits. This would permit the close apposition of two active centres of bacterial glutamate-5-phosphate reductase (the next enzyme in the proline/ornithine-synthesising route), supporting the postulated channelling of G5P. The structures clarify substrate binding and catalysis, justify the high glutamate specificity, explain the effects of known point mutations, and support the binding of proline near glutamate. Proline binding may trigger the movement of a loop that encircles glutamate, and which participates in a hydrogen bond network connecting active centres, which is possibly involved in the cooperativity for glutamate. © 2007 Elsevier Ltd. All rights reserved. |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007 2015 2015 2015 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Postprint info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/111016 |
| url |
http://hdl.handle.net/10261/111016 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
http://dx.doi.org/10.1016/j.jmb.2007.01.073 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Academic Press |
| publisher.none.fl_str_mv |
Academic Press |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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15.812429 |