Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.

Free Access at: http://glycob.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=20488940

Detalhes bibliográficos
Autores: Rey-Gallardo, Angela, Escribano, Cristina, Delgado-Martín, Cristina, Rodríguez-Fernández, José Luis, Gerardy-Schahn, Rita, Rutishauser, Urs, Corbí, Angel L., Vega Palacios, Miguel A.
Formato: artículo
Fecha de publicación:2010
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/41767
Acesso em linha:http://hdl.handle.net/10261/41767
Access Level:acceso abierto
Palavra-chave:Neuropilin-2
Dendritic cells
Chemokine CCL21
Chemotaxis
Polysialic acid
Polysialyltransferase ST8SiaIV
Lymphoid organs
Metastasis
id ES_8db55bfa8bd27b76f3d9566ea11ccf2b
oai_identifier_str oai:digital.csic.es:10261/41767
network_acronym_str ES
network_name_str España
repository_id_str
spelling Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.Rey-Gallardo, AngelaEscribano, CristinaDelgado-Martín, CristinaRodríguez-Fernández, José LuisGerardy-Schahn, RitaRutishauser, UrsCorbí, Angel L.Vega Palacios, Miguel A.Neuropilin-2Dendritic cellsChemokine CCL21ChemotaxisPolysialic acidPolysialyltransferase ST8SiaIVLymphoid organsMetastasisFree Access at: http://glycob.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=20488940Dendritic cell (DC) migration to secondary lymphoid organs is a critical step to properly exert its role in immunity; and predominantly depends on the interaction of the chemokine receptor CCR7 with its ligands CCL21 and CCL19. Polysialic acid (PSA) has been recently reported to control CCL21-directed migration of mature DCs. Here; we first demonstrate that PSA present on human mature monocyte-derived dendritic cells did not enhance chemotactic responses to CCL19. We have also explored the molecular mechanisms underlying the selective enhancing effect of PSA on CCL21-driven chemotaxis of DCs. In this regard; we found out that prevention of DC polysialylation decreased CCL21 activation of JNK and Akt signaling pathways; both associated with CCR7-mediated chemotaxis. We also report that the enhanced PSA-mediated effect on DC migration towards CCL21 relied on the highly basic C-terminal region of this chemokine; and depended on the PSA acceptor molecule neuropilin-2 (NRP2) and on the polysialyltransferase ST8SiaIV. Altogether; our data indicate that the CCR7/CCL21/NRP2/ST8SiaIV functional axis constitutes an important guidance clue for DC targeting to lymphoid organs.This work was supported by research grant from Fondo de Investigaciones Sanitarias, Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo (FISPI0708879 to MAV).Peer reviewedOxford University Press201120112010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/41767reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://glycob.oxfordjournals.org/content/20/9/1139.longinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/417672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
title Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
spellingShingle Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
Rey-Gallardo, Angela
Neuropilin-2
Dendritic cells
Chemokine CCL21
Chemotaxis
Polysialic acid
Polysialyltransferase ST8SiaIV
Lymphoid organs
Metastasis
title_short Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
title_full Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
title_fullStr Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
title_full_unstemmed Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
title_sort Polysialylated neuropilin-2 enhances human dendritic cell migration through the basic C-terminal region of CCL21.
dc.creator.none.fl_str_mv Rey-Gallardo, Angela
Escribano, Cristina
Delgado-Martín, Cristina
Rodríguez-Fernández, José Luis
Gerardy-Schahn, Rita
Rutishauser, Urs
Corbí, Angel L.
Vega Palacios, Miguel A.
author Rey-Gallardo, Angela
author_facet Rey-Gallardo, Angela
Escribano, Cristina
Delgado-Martín, Cristina
Rodríguez-Fernández, José Luis
Gerardy-Schahn, Rita
Rutishauser, Urs
Corbí, Angel L.
Vega Palacios, Miguel A.
author_role author
author2 Escribano, Cristina
Delgado-Martín, Cristina
Rodríguez-Fernández, José Luis
Gerardy-Schahn, Rita
Rutishauser, Urs
Corbí, Angel L.
Vega Palacios, Miguel A.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neuropilin-2
Dendritic cells
Chemokine CCL21
Chemotaxis
Polysialic acid
Polysialyltransferase ST8SiaIV
Lymphoid organs
Metastasis
topic Neuropilin-2
Dendritic cells
Chemokine CCL21
Chemotaxis
Polysialic acid
Polysialyltransferase ST8SiaIV
Lymphoid organs
Metastasis
description Free Access at: http://glycob.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=20488940
publishDate 2010
dc.date.none.fl_str_mv 2010
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/41767
url http://hdl.handle.net/10261/41767
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://glycob.oxfordjournals.org/content/20/9/1139.long
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413068525535232
score 15.811543

Registos relacionados