The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11

Seedlessness is greatly prized by consumers of fresh grapes. While stenospermocarpic seed abortion determined by the SEED DEVELOPMENT INHIBITOR (SDI) locus is the usual source of seedlessness in commercial grapevine (Vitis vinifera) cultivars, the underlying sdi mutation remains unknown. Here, we un...

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Autores: Royo, Carolina, Torres-Pérez, Rafael, Mauri, Nuria, Diestro Sánchez, Nieves, Cabezas Martínez, José Antonio, Marchal, Cécile, Lacombe, Thierry, Ibáñez Marcos, Javier, Tornel, Manuel, Carreño, Juan, Martínez-Zapater, José M., Carbonell-Bejerano, Pablo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193437
Acceso en línea:http://hdl.handle.net/10261/193437
Access Level:acceso abierto
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spelling The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11Royo, CarolinaTorres-Pérez, RafaelMauri, NuriaDiestro Sánchez, NievesCabezas Martínez, José AntonioMarchal, CécileLacombe, ThierryIbáñez Marcos, JavierTornel, ManuelCarreño, JuanMartínez-Zapater, José M.Carbonell-Bejerano, PabloSeedlessness is greatly prized by consumers of fresh grapes. While stenospermocarpic seed abortion determined by the SEED DEVELOPMENT INHIBITOR (SDI) locus is the usual source of seedlessness in commercial grapevine (Vitis vinifera) cultivars, the underlying sdi mutation remains unknown. Here, we undertook an integrative approach to identify the causal mutation. Quantitative genetics and fine-mapping in two ‘Crimson Seedless’-derived F1 mapping populations confirmed the major effect of the SDI locus and delimited the sdi mutation to a 323-kb region on chromosome 18. RNA-sequencing comparing seed traces of seedless and seeds of seeded F1 individuals identified processes triggered during sdi-determined seed abortion, including the activation of salicylic acid-dependent autoimmunity. The RNA-sequencing data set was investigated for candidate genes, and while no evidence for causal cis-acting regulatory mutations was detected, deleterious nucleotide changes in coding sequences of the seedless haplotype were predicted in two genes within the sdi fine-mapping interval. Targeted resequencing of the two genes in a collection of 124 grapevine cultivars showed that only the point variation causing the arginine-197-to-leucine substitution in the seed morphogenesis regulator gene AGAMOUS-LIKE11 (VviAGL11) was fully linked with stenospermocarpy. The concurrent postzygotic variation identified for this missense polymorphism and seedlessness phenotype in seeded somatic variants of the original stenospermocarpic cultivar supports a causal effect. We postulate that seed abortion caused by this amino acid substitution in VviAGL11 is the major cause of seedlessness in cultivated grapevine. This information can be exploited to boost seedless grape breeding.This work was partially supported by the European Union’s Plant Genomics ERA-Net project GRASP and by the Spanish Ministry of Economy (MINECO) projects BIO2011-026229 and BIO2014-59324-R. This work also benefited from networking activities within the funded European Cooperation in Science and Technology (COST) Action FA1106 QualityFruit.Peer reviewedAmerican Society of Plant BiologistsEuropean CommissionMinisterio de Economía y Competitividad (España)European Cooperation in Science and TechnologyTorres-Pérez, Rafael [0000-0002-3696-4720]Ibáñez Marcos, Javier [0000-0002-6286-5638]Cabezas, José Antonio [0000-0002-4728-6072]Carbonell-Bejerano, Pablo [0000-0002-7266-9665]Martínez-Zapater, José M. [0000-0001-7217-4454]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/193437reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-59324-Rhttp://dx.doi.org/10.1104/pp.18.00259Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1934372026-05-22T06:33:51Z
dc.title.none.fl_str_mv The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
title The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
spellingShingle The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
Royo, Carolina
title_short The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
title_full The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
title_fullStr The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
title_full_unstemmed The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
title_sort The major origin of seedless grapes is associated with a missense mutation in the MADS-box gene VviAGL11
dc.creator.none.fl_str_mv Royo, Carolina
Torres-Pérez, Rafael
Mauri, Nuria
Diestro Sánchez, Nieves
Cabezas Martínez, José Antonio
Marchal, Cécile
Lacombe, Thierry
Ibáñez Marcos, Javier
Tornel, Manuel
Carreño, Juan
Martínez-Zapater, José M.
Carbonell-Bejerano, Pablo
author Royo, Carolina
author_facet Royo, Carolina
Torres-Pérez, Rafael
Mauri, Nuria
Diestro Sánchez, Nieves
Cabezas Martínez, José Antonio
Marchal, Cécile
Lacombe, Thierry
Ibáñez Marcos, Javier
Tornel, Manuel
Carreño, Juan
Martínez-Zapater, José M.
Carbonell-Bejerano, Pablo
author_role author
author2 Torres-Pérez, Rafael
Mauri, Nuria
Diestro Sánchez, Nieves
Cabezas Martínez, José Antonio
Marchal, Cécile
Lacombe, Thierry
Ibáñez Marcos, Javier
Tornel, Manuel
Carreño, Juan
Martínez-Zapater, José M.
Carbonell-Bejerano, Pablo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
European Cooperation in Science and Technology
Torres-Pérez, Rafael [0000-0002-3696-4720]
Ibáñez Marcos, Javier [0000-0002-6286-5638]
Cabezas, José Antonio [0000-0002-4728-6072]
Carbonell-Bejerano, Pablo [0000-0002-7266-9665]
Martínez-Zapater, José M. [0000-0001-7217-4454]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Seedlessness is greatly prized by consumers of fresh grapes. While stenospermocarpic seed abortion determined by the SEED DEVELOPMENT INHIBITOR (SDI) locus is the usual source of seedlessness in commercial grapevine (Vitis vinifera) cultivars, the underlying sdi mutation remains unknown. Here, we undertook an integrative approach to identify the causal mutation. Quantitative genetics and fine-mapping in two ‘Crimson Seedless’-derived F1 mapping populations confirmed the major effect of the SDI locus and delimited the sdi mutation to a 323-kb region on chromosome 18. RNA-sequencing comparing seed traces of seedless and seeds of seeded F1 individuals identified processes triggered during sdi-determined seed abortion, including the activation of salicylic acid-dependent autoimmunity. The RNA-sequencing data set was investigated for candidate genes, and while no evidence for causal cis-acting regulatory mutations was detected, deleterious nucleotide changes in coding sequences of the seedless haplotype were predicted in two genes within the sdi fine-mapping interval. Targeted resequencing of the two genes in a collection of 124 grapevine cultivars showed that only the point variation causing the arginine-197-to-leucine substitution in the seed morphogenesis regulator gene AGAMOUS-LIKE11 (VviAGL11) was fully linked with stenospermocarpy. The concurrent postzygotic variation identified for this missense polymorphism and seedlessness phenotype in seeded somatic variants of the original stenospermocarpic cultivar supports a causal effect. We postulate that seed abortion caused by this amino acid substitution in VviAGL11 is the major cause of seedlessness in cultivated grapevine. This information can be exploited to boost seedless grape breeding.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193437
url http://hdl.handle.net/10261/193437
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-59324-R
http://dx.doi.org/10.1104/pp.18.00259
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologists
publisher.none.fl_str_mv American Society of Plant Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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