Quorum-driven microbial consortium for Bioplastic production from agro-waste

12 p.-4 fig.-3 tab.-1 graph. abst.

Detalhes bibliográficos
Autores: Crespo-Roche, Diego, Herráez, Marta, Guerrero-Flores, Javier, Martínez, María Jesús, Louie, Katherine B., Northen, Trent R., Prieto Orzanco, Alicia, Barriuso, Jorge
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/398675
Acesso em linha:http://hdl.handle.net/10261/398675
Access Level:acceso abierto
Palavra-chave:Polyhydroxyalkanoates
Breweries spent grain
Waste cooking oil
Ophiostoma
Pseudomonas
Quorum sensing
Farnesol
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repository_id_str
spelling Quorum-driven microbial consortium for Bioplastic production from agro-wasteCrespo-Roche, DiegoHerráez, MartaGuerrero-Flores, JavierMartínez, María JesúsLouie, Katherine B.Northen, Trent R.Prieto Orzanco, AliciaBarriuso, JorgePolyhydroxyalkanoatesBreweries spent grainWaste cooking oilOphiostomaPseudomonasQuorum sensingFarnesol12 p.-4 fig.-3 tab.-1 graph. abst.Microbial consortia have high relevance in natural environments. Here we present the production of polyhydroxyalkanoates (PHA) from agro-industrial residues by a synthetic interkingdom consortium formed by the saprotrophic fungus Ophiostoma piceae CECT 20146, which encodes a wide range of lignocellulolytic enzymes, and a natural PHA producer, Pseudomonas putida KT2440. Two agro-industrial residues were utilized: Brewer’s Spent Grain (BSG) as a carbon/nitrogen source and biofilm scaffold and waste cooking oil (WCO) as a carbon source for PHA synthesis. Through biochemistry, microscopy, and omics analyses, it is shown that P. putida accumulates up to 40.2% of intracellular PHA when the quorum sensing molecule, farnesol (naturally produced by O. piceae) is added, thanks to the increased proliferation of P. putida cells. An interactive Shiny application has also been developed for an easy visualization and comprehension of all the transcriptomics and metabolomics data: https://jgf-bioinformatics.shinyapps.io/Visualization_app/. These results support the increased PHA production of the consortium by an induction of gene phaG, which redirects intermediaries of the fatty acid biosynthesis to PHA precursors, and the repression of the PHA depolymerase phaZ in P. putida. The trophic interaction between microorganisms seems to rely on the citric acid produced by O. piceae and the glycerol liberated from WCO, which can both be consumed by P. putida. Bioreactor scale-up experiments allowed a 3.3-fold increase in the PHA concentration in the consortium (6.7 g·L–1) without pretreatment or sterilization of the substrates, laying the groundwork for the implementation of an industrial consolidated bioprocess (CBP).This work was supported by the Spanish projects FungiQuorum (BIO2015-73697-JIN MCIN/AEI/FEDER/UE) and MICODE (PID2020-114210RB-I00 MCIN/AEI). Diego Crespo thanks the Spanish Government for his FPU21/02874 fellowship. The transcriptomic and metabolomic work (proposal ID: 504782-CSP) was conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. The authors would also like to thank the IBISBA European infrastructure (https://ibisba.eu/), the SusPlast-CSIC Interdisciplinary Platform (http://www.susplast-support).Peer reviewedAmerican Chemical SocietyMinisterio de Ciencia e Innovación (España)Joint Genome Institute (US)Department of Energy (US)Consejo Superior de Investigaciones Científicas (España)Martínez, María Jesús [0000-0003-2166-1097]Louie, Katherine B. [0000-0002-6787-7558]Northen, Trent R. [0000-0001-8404-3259]Prieto Orzanco, Alicia [0000-0002-5075-4025]Barriuso, Jorge [0000-0003-0916-6560]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/398675reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//BIO2015-73697-JINinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114210RB-I00https://doi.org/10.1021/acssuschemeng.5c05453Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3986752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Quorum-driven microbial consortium for Bioplastic production from agro-waste
title Quorum-driven microbial consortium for Bioplastic production from agro-waste
spellingShingle Quorum-driven microbial consortium for Bioplastic production from agro-waste
Crespo-Roche, Diego
Polyhydroxyalkanoates
Breweries spent grain
Waste cooking oil
Ophiostoma
Pseudomonas
Quorum sensing
Farnesol
title_short Quorum-driven microbial consortium for Bioplastic production from agro-waste
title_full Quorum-driven microbial consortium for Bioplastic production from agro-waste
title_fullStr Quorum-driven microbial consortium for Bioplastic production from agro-waste
title_full_unstemmed Quorum-driven microbial consortium for Bioplastic production from agro-waste
title_sort Quorum-driven microbial consortium for Bioplastic production from agro-waste
dc.creator.none.fl_str_mv Crespo-Roche, Diego
Herráez, Marta
Guerrero-Flores, Javier
Martínez, María Jesús
Louie, Katherine B.
Northen, Trent R.
Prieto Orzanco, Alicia
Barriuso, Jorge
author Crespo-Roche, Diego
author_facet Crespo-Roche, Diego
Herráez, Marta
Guerrero-Flores, Javier
Martínez, María Jesús
Louie, Katherine B.
Northen, Trent R.
Prieto Orzanco, Alicia
Barriuso, Jorge
author_role author
author2 Herráez, Marta
Guerrero-Flores, Javier
Martínez, María Jesús
Louie, Katherine B.
Northen, Trent R.
Prieto Orzanco, Alicia
Barriuso, Jorge
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Joint Genome Institute (US)
Department of Energy (US)
Consejo Superior de Investigaciones Científicas (España)
Martínez, María Jesús [0000-0003-2166-1097]
Louie, Katherine B. [0000-0002-6787-7558]
Northen, Trent R. [0000-0001-8404-3259]
Prieto Orzanco, Alicia [0000-0002-5075-4025]
Barriuso, Jorge [0000-0003-0916-6560]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Polyhydroxyalkanoates
Breweries spent grain
Waste cooking oil
Ophiostoma
Pseudomonas
Quorum sensing
Farnesol
topic Polyhydroxyalkanoates
Breweries spent grain
Waste cooking oil
Ophiostoma
Pseudomonas
Quorum sensing
Farnesol
description 12 p.-4 fig.-3 tab.-1 graph. abst.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/398675
url http://hdl.handle.net/10261/398675
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//BIO2015-73697-JIN
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114210RB-I00
https://doi.org/10.1021/acssuschemeng.5c05453
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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