Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans

Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans; increased colonization of this yeast in this niche has implicated the master regulator of the white-opaque transition, Wor1, by mechanisms not completely understood. We have addressed the role that this transcr...

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Autores: Hidalgo Vico, Susana, Casas, Josefina, García, Carolina, Lillo, M Pilar, Alonso Monge, Rebeca María Del Mar, Román González, Elvira, Pla Alonso, Jesús
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/128924
Acesso em linha:https://hdl.handle.net/20.500.14352/128924
Access Level:acceso abierto
Palavra-chave:615.28
commensalism
gut
lipid
mitochondrial activity
reactive oxygen species
Candida albicans
Wor1
Microbiología (Farmacia)
2414 Microbiología
2415.01 Biología Molecular de Microorganismos
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oai_identifier_str oai:docta.ucm.es:20.500.14352/128924
network_acronym_str ES
network_name_str España
repository_id_str
spelling Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicansHidalgo Vico, SusanaCasas, JosefinaGarcía, CarolinaLillo, M PilarAlonso Monge, Rebeca María Del MarRomán González, ElviraPla Alonso, Jesús615.28commensalismgutlipidmitochondrial activityreactive oxygen speciesCandida albicansWor1Microbiología (Farmacia)2414 Microbiología2415.01 Biología Molecular de MicroorganismosCandida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans; increased colonization of this yeast in this niche has implicated the master regulator of the white-opaque transition, Wor1, by mechanisms not completely understood. We have addressed the role that this transcription factor has on commensalism by the characterization of strains overexpressing this gene. We show that WOR1 overexpression causes an alteration of the total lipid content of the fungal cell and significantly alters the composition of structural and reserve molecular species lipids as determined by lipidomic analysis. These cells are hypersensitive to membrane-disturbing agents such as SDS, have increased tolerance to azoles, an augmented number of peroxisomes, and increased phospholipase activity. WOR1 overexpression also decreases mitochondrial activity and results in altered susceptibility to certain oxidants. All together, these changes reflect drastic alterations in the cellular physiology that facilitate adaptation to the gastrointestinal tract environment.MDPIUniversidad Complutense de Madrid20222022-09-2820222022-09-28journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/128924reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1289242026-06-02T12:44:21Z
dc.title.none.fl_str_mv Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
title Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
spellingShingle Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
Hidalgo Vico, Susana
615.28
commensalism
gut
lipid
mitochondrial activity
reactive oxygen species
Candida albicans
Wor1
Microbiología (Farmacia)
2414 Microbiología
2415.01 Biología Molecular de Microorganismos
title_short Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
title_full Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
title_fullStr Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
title_full_unstemmed Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
title_sort Overexpression of the White Opaque Switching Master Regulator Wor1 Alters Lipid Metabolism and Mitochondrial Function in Candida albicans
dc.creator.none.fl_str_mv Hidalgo Vico, Susana
Casas, Josefina
García, Carolina
Lillo, M Pilar
Alonso Monge, Rebeca María Del Mar
Román González, Elvira
Pla Alonso, Jesús
author Hidalgo Vico, Susana
author_facet Hidalgo Vico, Susana
Casas, Josefina
García, Carolina
Lillo, M Pilar
Alonso Monge, Rebeca María Del Mar
Román González, Elvira
Pla Alonso, Jesús
author_role author
author2 Casas, Josefina
García, Carolina
Lillo, M Pilar
Alonso Monge, Rebeca María Del Mar
Román González, Elvira
Pla Alonso, Jesús
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.28
commensalism
gut
lipid
mitochondrial activity
reactive oxygen species
Candida albicans
Wor1
Microbiología (Farmacia)
2414 Microbiología
2415.01 Biología Molecular de Microorganismos
topic 615.28
commensalism
gut
lipid
mitochondrial activity
reactive oxygen species
Candida albicans
Wor1
Microbiología (Farmacia)
2414 Microbiología
2415.01 Biología Molecular de Microorganismos
description Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans; increased colonization of this yeast in this niche has implicated the master regulator of the white-opaque transition, Wor1, by mechanisms not completely understood. We have addressed the role that this transcription factor has on commensalism by the characterization of strains overexpressing this gene. We show that WOR1 overexpression causes an alteration of the total lipid content of the fungal cell and significantly alters the composition of structural and reserve molecular species lipids as determined by lipidomic analysis. These cells are hypersensitive to membrane-disturbing agents such as SDS, have increased tolerance to azoles, an augmented number of peroxisomes, and increased phospholipase activity. WOR1 overexpression also decreases mitochondrial activity and results in altered susceptibility to certain oxidants. All together, these changes reflect drastic alterations in the cellular physiology that facilitate adaptation to the gastrointestinal tract environment.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-09-28
2022
2022-09-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/128924
url https://hdl.handle.net/20.500.14352/128924
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413051791310848
score 15.812429

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