Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions

In this study, we explored the biocompatibility of Au nanoparticles (NPs) capped with peptide-biphenyl hybrid (PBH) ligands containing glycine (Gly), cysteine (Cys), tyrosine (Tyr), tryptophan (Trp) and methionine (Met) amino acids in the human hepatocellular carcinoma cell line Hep G2. Five AuNPs,...

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Detalles Bibliográficos
Autores: Connoly, Mona, Pérez, Yolanda, Mann, Enrique, Herradón García, Bernardo, Fernández-Cruz, M. L., Navas, José María
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/292393
Acceso en línea:http://hdl.handle.net/10261/292393
Access Level:acceso abierto
Palabra clave:Gold nanoparticles
Hep G2
ROS
Autophagy
Cytotoxicity
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spelling Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditionsConnoly, MonaPérez, YolandaMann, EnriqueHerradón García, BernardoFernández-Cruz, M. L.Navas, José MaríaGold nanoparticlesHep G2ROSAutophagyCytotoxicityIn this study, we explored the biocompatibility of Au nanoparticles (NPs) capped with peptide-biphenyl hybrid (PBH) ligands containing glycine (Gly), cysteine (Cys), tyrosine (Tyr), tryptophan (Trp) and methionine (Met) amino acids in the human hepatocellular carcinoma cell line Hep G2. Five AuNPs, Au[(Met)2B], Au[(Gly-Tyr-TrCys)2B], Au[(Met)2B], Au[(Gly-Tyr-TrCys)2B] and Au[(TrCys)2B], were synthesised. Physico-chemical and cytotoxic properties were thoroughly studied. Transmission electron micrographs showed isolated near-spherical nanoparticles with diameters of 1.5, 1.6, 2.3, 1.8 and 2.3 nm, respectively. Dynamic light scattering evidenced the high stability of suspensions in Milli-Q water and culture medium, particularly when supplemented with serum, showing in all cases a tendency to form agglomerates with diameters approximately 200 nm. In the cytotoxicity studies, interference caused by AuNPs with some typical cytotoxicity assays was demonstrated; thus, only data obtained from the resazurin based assay were used. After 48-h incubation, only concentrations ≥50 μg/ml exhibited cytotoxicity. Such doses were also responsible for an increase in reactive oxygen species (ROS). Some differences were observed among the studied NPs. Of particular importance is the AuNPs capped with the PBH ligand (Gly-Tyr-TrCys)2B showing remarkable stability in culture medium, even in the absence of serum. Moreover, these AuNPs have unique biological effects on Hep G2 cells while showing low toxicity. The production of ROS along with supporting optical microscopy images suggests cellular interaction/uptake of these particular AuNPs. Future research efforts should further test this hypothesis, as such interaction/uptake is highly relevant in drug delivery systems. © 2013 Connolly et al.Peer reviewedSpringer NatureConnoly, Mona [0000-0003-3574-8564]Fernández-Cruz, M. L. [0000-0001-5988-1939]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/292393reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDepartamento de Medio Ambiente y AgronomíaSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2923932026-05-22T06:33:51Z
dc.title.none.fl_str_mv Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
title Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
spellingShingle Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
Connoly, Mona
Gold nanoparticles
Hep G2
ROS
Autophagy
Cytotoxicity
title_short Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
title_full Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
title_fullStr Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
title_full_unstemmed Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
title_sort Peptide-biphenyl hybrid-capped AuNPs Stability and biocompatibility under cell culture conditions
dc.creator.none.fl_str_mv Connoly, Mona
Pérez, Yolanda
Mann, Enrique
Herradón García, Bernardo
Fernández-Cruz, M. L.
Navas, José María
author Connoly, Mona
author_facet Connoly, Mona
Pérez, Yolanda
Mann, Enrique
Herradón García, Bernardo
Fernández-Cruz, M. L.
Navas, José María
author_role author
author2 Pérez, Yolanda
Mann, Enrique
Herradón García, Bernardo
Fernández-Cruz, M. L.
Navas, José María
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Connoly, Mona [0000-0003-3574-8564]
Fernández-Cruz, M. L. [0000-0001-5988-1939]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gold nanoparticles
Hep G2
ROS
Autophagy
Cytotoxicity
topic Gold nanoparticles
Hep G2
ROS
Autophagy
Cytotoxicity
description In this study, we explored the biocompatibility of Au nanoparticles (NPs) capped with peptide-biphenyl hybrid (PBH) ligands containing glycine (Gly), cysteine (Cys), tyrosine (Tyr), tryptophan (Trp) and methionine (Met) amino acids in the human hepatocellular carcinoma cell line Hep G2. Five AuNPs, Au[(Met)2B], Au[(Gly-Tyr-TrCys)2B], Au[(Met)2B], Au[(Gly-Tyr-TrCys)2B] and Au[(TrCys)2B], were synthesised. Physico-chemical and cytotoxic properties were thoroughly studied. Transmission electron micrographs showed isolated near-spherical nanoparticles with diameters of 1.5, 1.6, 2.3, 1.8 and 2.3 nm, respectively. Dynamic light scattering evidenced the high stability of suspensions in Milli-Q water and culture medium, particularly when supplemented with serum, showing in all cases a tendency to form agglomerates with diameters approximately 200 nm. In the cytotoxicity studies, interference caused by AuNPs with some typical cytotoxicity assays was demonstrated; thus, only data obtained from the resazurin based assay were used. After 48-h incubation, only concentrations ≥50 μg/ml exhibited cytotoxicity. Such doses were also responsible for an increase in reactive oxygen species (ROS). Some differences were observed among the studied NPs. Of particular importance is the AuNPs capped with the PBH ligand (Gly-Tyr-TrCys)2B showing remarkable stability in culture medium, even in the absence of serum. Moreover, these AuNPs have unique biological effects on Hep G2 cells while showing low toxicity. The production of ROS along with supporting optical microscopy images suggests cellular interaction/uptake of these particular AuNPs. Future research efforts should further test this hypothesis, as such interaction/uptake is highly relevant in drug delivery systems. © 2013 Connolly et al.
publishDate 2013
dc.date.none.fl_str_mv 2013
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/292393
url http://hdl.handle.net/10261/292393
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Departamento de Medio Ambiente y Agronomía
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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