Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity
9 Pág.
| Autores: | , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/337290 |
| Acceso en línea: | http://hdl.handle.net/10261/337290 https://api.elsevier.com/content/abstract/scopus_id/85090664925 |
| Access Level: | acceso abierto |
| Palabra clave: | DNA integrity Sperm cryoresistance Valine |
| id |
ES_8d0a28fa3419b9c5068c1e69f483645f |
|---|---|
| oai_identifier_str |
oai:digital.csic.es:10261/337290 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity |
| title |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity |
| spellingShingle |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity Bernal Juárez, Berenice DNA integrity Sperm cryoresistance Valine |
| title_short |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity |
| title_full |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity |
| title_fullStr |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity |
| title_full_unstemmed |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity |
| title_sort |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacity |
| dc.creator.none.fl_str_mv |
Bernal Juárez, Berenice Iglesias-Cabeza, Nerea Sánchez-Rivera, U. Toledano-Díaz, Adolfo Castaño, Cristina Pérez Cerezales, Serafín Gutiérrez-Adán, Alfonso López Sebastián, Antonio García-Casado, Pedro García-Gil, María Woelders, Henri Blesbois, E. Santiago-Moreno, Julián |
| author |
Bernal Juárez, Berenice |
| author_facet |
Bernal Juárez, Berenice Iglesias-Cabeza, Nerea Sánchez-Rivera, U. Toledano-Díaz, Adolfo Castaño, Cristina Pérez Cerezales, Serafín Gutiérrez-Adán, Alfonso López Sebastián, Antonio García-Casado, Pedro García-Gil, María Woelders, Henri Blesbois, E. Santiago-Moreno, Julián |
| author_role |
author |
| author2 |
Iglesias-Cabeza, Nerea Sánchez-Rivera, U. Toledano-Díaz, Adolfo Castaño, Cristina Pérez Cerezales, Serafín Gutiérrez-Adán, Alfonso López Sebastián, Antonio García-Casado, Pedro García-Gil, María Woelders, Henri Blesbois, E. Santiago-Moreno, Julián |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
European Commission CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Bernal Juárez, Berenice [0000-0001-9144-1554] Iglesias-Cabeza, Nerea [0000-0001-8775-155X] Sánchez-Rivera, U. [0000-0002-9423-5278] Toledano Díaz, Adolfo [0000-0001-6679-485X] Castaño, Cristina [0000-0003-1134-1436] Pérez Cerezales, Serafín [0000-0003-2119-6228] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] López Sebastián, Antonio [0000-0001-8695-7441] García-Casado, Pedro [0000-0002-1242-1035] García Gil, M. [0000-0003-2856-277X] Santiago Moreno, Julián [0000-0001-5551-8120] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
DNA integrity Sperm cryoresistance Valine |
| topic |
DNA integrity Sperm cryoresistance Valine |
| description |
9 Pág. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/337290 https://api.elsevier.com/content/abstract/scopus_id/85090664925 |
| url |
http://hdl.handle.net/10261/337290 https://api.elsevier.com/content/abstract/scopus_id/85090664925 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/677353 info:eu-repo/grantAgreement/CSIC-INIA//RZP2015-0002-00-00 Departamento de Reproducción Animal https://doi.org/10.1016/j.psj.2020.09.060 Sí |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869412997119606784 |
| spelling |
Effect of supplementation of valine to chicken extender on sperm cryoresistance and post-thaw fertilization capacityBernal Juárez, BereniceIglesias-Cabeza, NereaSánchez-Rivera, U.Toledano-Díaz, AdolfoCastaño, CristinaPérez Cerezales, SerafínGutiérrez-Adán, AlfonsoLópez Sebastián, AntonioGarcía-Casado, PedroGarcía-Gil, MaríaWoelders, HenriBlesbois, E.Santiago-Moreno, JuliánDNA integritySperm cryoresistanceValine9 Pág.Recent reports showed a positive correlation between frozen-thawed rooster sperm DNA integrity and the concentrations of valine in seminal plasma. The present study evaluated the effect of supplementing valine to semen extender for freezing sperm of 2 endangered local Spanish chicken breeds with different sperm cryoresistance: Red Villafranquina (VF) showing low sperm DNA integrity after cryopreservation and Quail Castellana that shows higher DNA integrity. One pool of semen per breed was obtained twice a week for 10 wk (n = 40, 20 per breed). Each pool was divided into 2 fractions. One of these fractions was frozen in presence of valine as additive in the extender (concentration 10 mmol), whereas the other was used as control. The evaluation of the samples before and after freezing-thawing included motility (CASA-Mot system), viability (propidium iodide and SYBR-14), DNA integrity (terminal deoxynucleotidyl transferase dUTP nick end labeling), and fertility rate (percentage of eggs with blastoderm development after artificial insemination). Supplementation of valine increased several motility variables of fresh semen. In VF breed, valine increased percentage of progressive motile sperm (P = 0.025), curvilinear velocity (P = 0.033), straight-line velocity (P = 0.040), and average path velocity (P = 0.033), whereas progressive motile sperm (P = 0.019), curvilinear velocity (P = 0.006), straight-line velocity (P = 0.003) and average path velocity (P = 0.004) were improved in the Quail Castellana breed. Valine addition increased the DNA integrity of cryopreserved semen (decreased post-thaw DNA fragmentation) in both breeds, with a significant effect (P = 0.002) in VF (36.3% VF-control vs 31%VF-valine). As expected, Quail Castellana cryopreserved sperm control showed higher fertility rate (34.4% ± 12.1) than VF cryopreserved sperm control (16.1% + 6.2). Supplementing valine to the freezing extender doubled (P = 0.026) the fertility rate of VF (32.6% ± 12.2) compared with the control (16.1% + 6.2). In conclusion, supplementation of valine to chicken freezing extenders shows a positive effect on DNA fragmentation and fertilizing ability of frozen-thawed sperm, with a better response in a breed considered as the lowest freezer in our conservatory.This paper is dedicated to the memory of our dear co-worker, Dr. Serafín Pérez Cerezales, who recently passed-away. Serafín took part in the DNA analyses, and in the critical revision of the original manuscript. This research is part of a project that received funding from the European Union's Horizon 2020 Research and Innovation Programme under grant agreement Nº 677353 IMAGE. Part of this research was funded by Zoitechlab S.L.-INIA contract CON 18-141, and INIA project RZP2015-0002-00-00.Peer reviewedElsevierEuropean CommissionCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Bernal Juárez, Berenice [0000-0001-9144-1554]Iglesias-Cabeza, Nerea [0000-0001-8775-155X]Sánchez-Rivera, U. [0000-0002-9423-5278]Toledano Díaz, Adolfo [0000-0001-6679-485X]Castaño, Cristina [0000-0003-1134-1436]Pérez Cerezales, Serafín [0000-0003-2119-6228]Gutiérrez-Adán, Alfonso [0000-0001-9893-9179]López Sebastián, Antonio [0000-0001-8695-7441]García-Casado, Pedro [0000-0002-1242-1035]García Gil, M. [0000-0003-2856-277X]Santiago Moreno, Julián [0000-0001-5551-8120]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/337290https://api.elsevier.com/content/abstract/scopus_id/85090664925reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/677353info:eu-repo/grantAgreement/CSIC-INIA//RZP2015-0002-00-00Departamento de Reproducción Animalhttps://doi.org/10.1016/j.psj.2020.09.060Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3372902026-05-22T06:33:51Z |
| score |
15,811543 |