Chromosomal integrons are genetically and functionally isolated units of genomes

Supplementary Data are available at NAR Online.

Detalles Bibliográficos
Autores: Blanco, Paula, Trigo da Roza, Filipa, Toribio-Celestino, Laura, García-Pastor, Lucía, Caselli, Niccolò, Morón, Álvaro, Ojeda, Francisco, Darracq, Baptiste, Vergara, Ester, Amaro, Francisco, San Millán, Álvaro, Skovgaard, Ole, Mazel, Didier, Loot, Céline, Escudero, José Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/373778
Acceso en línea:http://hdl.handle.net/10261/373778
https://api.elsevier.com/content/abstract/scopus_id/85208772753
Access Level:acceso abierto
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spelling Chromosomal integrons are genetically and functionally isolated units of genomesBlanco, PaulaTrigo da Roza, FilipaToribio-Celestino, LauraGarcía-Pastor, LucíaCaselli, NiccolòMorón, ÁlvaroOjeda, FranciscoDarracq, BaptisteVergara, EsterAmaro, FranciscoSan Millán, ÁlvaroSkovgaard, OleMazel, DidierLoot, CélineEscudero, José AntonioSupplementary Data are available at NAR Online.Integrons are genetic elements that increase the evolvability of bacteria by capturing new genes and stockpiling them in arrays. Sedentary chromosomal integrons (SCIs) can be massive and highly stabilized structures encoding hundreds of genes, whose function remains generally unknown. SCIs have co-evolved with the host for aeons and are highly intertwined with their physiology from a mechanistic point of view. But, paradoxically, other aspects, like their variable content and location within the genome, suggest a high genetic and functional independence. In this work, we have explored the connection of SCIs to their host genome using as a model the Superintegron (SI), a 179-cassette long SCI in the genome of Vibrio cholerae N16961. We have relocated and deleted the SI using SeqDelTA, a novel method that allows to counteract the strong stabilization conferred by toxin-antitoxin systems within the array. We have characterized in depth the impact in V. cholerae's physiology, measuring fitness, chromosome replication dynamics, persistence, transcriptomics, phenomics, natural competence, virulence and resistance against protist grazing. The deletion of the SI did not produce detectable effects in any condition, proving that-despite millions of years of co-evolution-SCIs are genetically and functionally isolated units of genomes.European Research Council (ERC) [803375-KRYPTONINT; 757440-PLASREVOLUTION] to J.A.E, and L.T.C. and A.S.M.; Ministerio de Ciencia, Innovación y Universidades [BIO2017-85056-P, PID2020-117499RB-100] to J.A.E.; Comunidad de Madrid [2016-T1/BIO-1105, 2020-5A/BIO-19726 to J.A.E.]; Ministerio de Ciencia, Innovación y Universidades[FJC2020-043017-I] toP.B.;Fundação para Ciência e a Tecnologia [SFRH/BD/144108/2019] to F.T.R.; Centre National de la Recherche Scientifique [CNRS-UMR3525] to B.D., D.M., C.L.; Fondation pour la Recherche Médicale [EQU202103012569] to D.M.. Funding for open access charge: European Research Council Starting [803375].Peer reviewedOxford University PressEuropean Research CouncilMinisterio de Ciencia, Innovación y Universidades (España)Fundação para a Ciência e a Tecnologia (Portugal)Centre National de la Recherche Scientifique (France)Fondation pour la Recherche MédicaleComunidad de MadridToribio-Celestino, Laura [0000-0002-8848-1691]San Millán, Álvaro [0000-0001-8544-0387]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/373778https://api.elsevier.com/content/abstract/scopus_id/85208772753reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-85056-Pinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117499RB-I00Nucleic acids researchapplication/pdfhttps://doi.org/10.1093/nar/gkae866Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3737782026-05-22T06:33:51Z
dc.title.none.fl_str_mv Chromosomal integrons are genetically and functionally isolated units of genomes
title Chromosomal integrons are genetically and functionally isolated units of genomes
spellingShingle Chromosomal integrons are genetically and functionally isolated units of genomes
Blanco, Paula
title_short Chromosomal integrons are genetically and functionally isolated units of genomes
title_full Chromosomal integrons are genetically and functionally isolated units of genomes
title_fullStr Chromosomal integrons are genetically and functionally isolated units of genomes
title_full_unstemmed Chromosomal integrons are genetically and functionally isolated units of genomes
title_sort Chromosomal integrons are genetically and functionally isolated units of genomes
dc.creator.none.fl_str_mv Blanco, Paula
Trigo da Roza, Filipa
Toribio-Celestino, Laura
García-Pastor, Lucía
Caselli, Niccolò
Morón, Álvaro
Ojeda, Francisco
Darracq, Baptiste
Vergara, Ester
Amaro, Francisco
San Millán, Álvaro
Skovgaard, Ole
Mazel, Didier
Loot, Céline
Escudero, José Antonio
author Blanco, Paula
author_facet Blanco, Paula
Trigo da Roza, Filipa
Toribio-Celestino, Laura
García-Pastor, Lucía
Caselli, Niccolò
Morón, Álvaro
Ojeda, Francisco
Darracq, Baptiste
Vergara, Ester
Amaro, Francisco
San Millán, Álvaro
Skovgaard, Ole
Mazel, Didier
Loot, Céline
Escudero, José Antonio
author_role author
author2 Trigo da Roza, Filipa
Toribio-Celestino, Laura
García-Pastor, Lucía
Caselli, Niccolò
Morón, Álvaro
Ojeda, Francisco
Darracq, Baptiste
Vergara, Ester
Amaro, Francisco
San Millán, Álvaro
Skovgaard, Ole
Mazel, Didier
Loot, Céline
Escudero, José Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Research Council
Ministerio de Ciencia, Innovación y Universidades (España)
Fundação para a Ciência e a Tecnologia (Portugal)
Centre National de la Recherche Scientifique (France)
Fondation pour la Recherche Médicale
Comunidad de Madrid
Toribio-Celestino, Laura [0000-0002-8848-1691]
San Millán, Álvaro [0000-0001-8544-0387]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Supplementary Data are available at NAR Online.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/373778
https://api.elsevier.com/content/abstract/scopus_id/85208772753
url http://hdl.handle.net/10261/373778
https://api.elsevier.com/content/abstract/scopus_id/85208772753
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-85056-P
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-117499RB-I00
Nucleic acids research
application/pdf
https://doi.org/10.1093/nar/gkae866
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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