Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system

Copyright © by Portland Press. The final version of record is available at http://www.biochemj.org/bj/default.htm

Bibliographic Details
Authors: Sanz, Libia, Bazaa, Amine, Marrakchi, Naziha, Pérez, Alicia, Chenik, Mehdi, Bel Lasfer, Zakaria, El Ayeb, Mohamed, Calvete, Juan J.
Format: article
Publication Date:2006
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/3589
Online Access:http://hdl.handle.net/10261/3589
Access Level:Open access
Keyword:Cerastes vipera
Macrovipera lebetina transmediterranea
Integrin inhibition
Snake venom disintegrin
Snake venomics
Toxin evolution
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spelling Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition systemSanz, LibiaBazaa, AmineMarrakchi, NazihaPérez, AliciaChenik, MehdiBel Lasfer, ZakariaEl Ayeb, MohamedCalvete, Juan J.Cerastes viperaMacrovipera lebetina transmediterraneaIntegrin inhibitionSnake venom disintegrinSnake venomicsToxin evolutionCopyright © by Portland Press. The final version of record is available at http://www.biochemj.org/bj/default.htmWe report the cloning and sequence analysis of Cerastes vipera and Macrovipera lebetina transmediterranea cDNAs coding for short non-RGD (Arg-Gly-Asp) disintegrins and for dimeric disintegrin subunits. The mRNAs belong to the short-coding class, suggesting that these disintegrin mRNAs may be more widely distributed than previously thought. Our data also argue for a common ancestry of the mRNAs of short disintegrins and those coding for precursors of dimeric disintegrin chains. The Macrovipera lebetina transmediterranea dimeric disintegrin reported to inhibit the laminin-binding integrins α3β1, α6β1 and α7β1 was analysed using a proteomic approach and was shown to bear MLD (Met-Leu-Asp) and VGD (Val-Gly-Asp) motifs. The results highlight the fact that disintegrins have evolved a restricted panel of integrin-blocking sequences that segregate with defined branches of the phylogenetic tree of the integrin α-chains, providing novel insights into the evolutionary adaptation of the snake venom antagonists to the ligand-binding sites of their target integrin receptors.This study has been partly financed by grant BFU2004-01432/BMC from the Ministerio de Educación y Ciencia, Madrid, Spain, and by the Spanish–Tunisian Cooperation Programme 29p/02 from the Agencia Española de Cooperación Internacional (AECI).Peer reviewedBiochemical Society200820082006info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501763802 bytesapplication/pdfhttp://hdl.handle.net/10261/3589reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/35892026-05-22T06:33:51Z
dc.title.none.fl_str_mv Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
title Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
spellingShingle Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
Sanz, Libia
Cerastes vipera
Macrovipera lebetina transmediterranea
Integrin inhibition
Snake venom disintegrin
Snake venomics
Toxin evolution
title_short Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
title_full Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
title_fullStr Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
title_full_unstemmed Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
title_sort Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
dc.creator.none.fl_str_mv Sanz, Libia
Bazaa, Amine
Marrakchi, Naziha
Pérez, Alicia
Chenik, Mehdi
Bel Lasfer, Zakaria
El Ayeb, Mohamed
Calvete, Juan J.
author Sanz, Libia
author_facet Sanz, Libia
Bazaa, Amine
Marrakchi, Naziha
Pérez, Alicia
Chenik, Mehdi
Bel Lasfer, Zakaria
El Ayeb, Mohamed
Calvete, Juan J.
author_role author
author2 Bazaa, Amine
Marrakchi, Naziha
Pérez, Alicia
Chenik, Mehdi
Bel Lasfer, Zakaria
El Ayeb, Mohamed
Calvete, Juan J.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cerastes vipera
Macrovipera lebetina transmediterranea
Integrin inhibition
Snake venom disintegrin
Snake venomics
Toxin evolution
topic Cerastes vipera
Macrovipera lebetina transmediterranea
Integrin inhibition
Snake venom disintegrin
Snake venomics
Toxin evolution
description Copyright © by Portland Press. The final version of record is available at http://www.biochemj.org/bj/default.htm
publishDate 2006
dc.date.none.fl_str_mv 2006
2008
2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/3589
url http://hdl.handle.net/10261/3589
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 763802 bytes
application/pdf
dc.publisher.none.fl_str_mv Biochemical Society
publisher.none.fl_str_mv Biochemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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