Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice
Diarrhetic shellfish toxins (DSTs) and phytoplankton species producing them have been frequently reported in seawater worldwide. They accumulate in shellfish and pose a significant threat to the environment and the health of seafood consumers. The goal of this study was to evaluate the acute toxicit...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad de Santiago de Compostela (USC) |
| Repositorio: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| Idioma: | inglés |
| OAI Identifier: | oai:minerva.usc.gal:10347/44224 |
| Acceso en línea: | https://hdl.handle.net/10347/44224 |
| Access Level: | acceso abierto |
| Palabra clave: | Diarrhetic shellfish poisoning (DSP) Okadaic acid Oral toxicity Voluntary feeding |
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Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in miceRodríguez Santos, LuisLouzao Ojeda, María del CarmenCostas Sánchez, CeliaCagide, EvaÁlvarez, MercedesGraña, AlmudenaCarrera González, María CristinaVale González, María del CarmenVilariño del Río, NataliaRodríguez Vieytes, MercedesLolo, ManuelBotana López, Luis MiguelDiarrhetic shellfish poisoning (DSP)Okadaic acidOral toxicityVoluntary feedingDiarrhetic shellfish toxins (DSTs) and phytoplankton species producing them have been frequently reported in seawater worldwide. They accumulate in shellfish and pose a significant threat to the environment and the health of seafood consumers. The goal of this study was to evaluate the acute toxicity of DSTs administered to mice via voluntary ingestion through bread. This is a method for the delivery of DSTs that provided convenient dosing, reduced animal stress and mimicked human exposure more accurately than oral gavage administration. Over 24 h, key parameters including clinical signs were meticulously recorded. The results showed a dose-dependent reduction in food and water consumption and significant relative body weight loss, with OA and DTX1 inducing more pronounced gastrointestinal disturbances at lower doses than DTX2. This is the first study to compare toxicity of DSTs administered by voluntary feeding where diarrhea is the main symptom. Effective dose 50 for watery diarrhea in the first 3 h highlighted that DTX2 is significantly less toxic than OA and DTX1. These insights enhance our comprehension of DTXs toxicity and contribute to the risk assessment of the dietary exposure of DSTs form shellfish.ElsevierUniversidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía FarmacéuticaUniversidade de Santiago de Compostela. Departamento de Fisioloxía20252025-09-1520252025-09-15journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/44224reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 CPP2021-008447 Evaluación de los efectos de las toxinas diarreicas de mariscos (DSP) tras una ingesta continuada y sus implicaciones neurológicas (CRONIC-DSP)Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2023-149618OB-I00 PAPEL DE LAS CICLOFILINAS EN LA INFLAMACION Y LA ATEROSCLEROSIS. MODULACION FARMACOLOGICA CON MOLECULAS GUIA DE ORIGEN MARINOopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/442242026-06-15T12:47:27Z |
| dc.title.none.fl_str_mv |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice |
| title |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice |
| spellingShingle |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice Rodríguez Santos, Luis Diarrhetic shellfish poisoning (DSP) Okadaic acid Oral toxicity Voluntary feeding |
| title_short |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice |
| title_full |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice |
| title_fullStr |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice |
| title_full_unstemmed |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice |
| title_sort |
Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice |
| dc.creator.none.fl_str_mv |
Rodríguez Santos, Luis Louzao Ojeda, María del Carmen Costas Sánchez, Celia Cagide, Eva Álvarez, Mercedes Graña, Almudena Carrera González, María Cristina Vale González, María del Carmen Vilariño del Río, Natalia Rodríguez Vieytes, Mercedes Lolo, Manuel Botana López, Luis Miguel |
| author |
Rodríguez Santos, Luis |
| author_facet |
Rodríguez Santos, Luis Louzao Ojeda, María del Carmen Costas Sánchez, Celia Cagide, Eva Álvarez, Mercedes Graña, Almudena Carrera González, María Cristina Vale González, María del Carmen Vilariño del Río, Natalia Rodríguez Vieytes, Mercedes Lolo, Manuel Botana López, Luis Miguel |
| author_role |
author |
| author2 |
Louzao Ojeda, María del Carmen Costas Sánchez, Celia Cagide, Eva Álvarez, Mercedes Graña, Almudena Carrera González, María Cristina Vale González, María del Carmen Vilariño del Río, Natalia Rodríguez Vieytes, Mercedes Lolo, Manuel Botana López, Luis Miguel |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica Universidade de Santiago de Compostela. Departamento de Fisioloxía |
| dc.subject.none.fl_str_mv |
Diarrhetic shellfish poisoning (DSP) Okadaic acid Oral toxicity Voluntary feeding |
| topic |
Diarrhetic shellfish poisoning (DSP) Okadaic acid Oral toxicity Voluntary feeding |
| description |
Diarrhetic shellfish toxins (DSTs) and phytoplankton species producing them have been frequently reported in seawater worldwide. They accumulate in shellfish and pose a significant threat to the environment and the health of seafood consumers. The goal of this study was to evaluate the acute toxicity of DSTs administered to mice via voluntary ingestion through bread. This is a method for the delivery of DSTs that provided convenient dosing, reduced animal stress and mimicked human exposure more accurately than oral gavage administration. Over 24 h, key parameters including clinical signs were meticulously recorded. The results showed a dose-dependent reduction in food and water consumption and significant relative body weight loss, with OA and DTX1 inducing more pronounced gastrointestinal disturbances at lower doses than DTX2. This is the first study to compare toxicity of DSTs administered by voluntary feeding where diarrhea is the main symptom. Effective dose 50 for watery diarrhea in the first 3 h highlighted that DTX2 is significantly less toxic than OA and DTX1. These insights enhance our comprehension of DTXs toxicity and contribute to the risk assessment of the dietary exposure of DSTs form shellfish. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025-09-15 2025 2025-09-15 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10347/44224 |
| url |
https://hdl.handle.net/10347/44224 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 CPP2021-008447 Evaluación de los efectos de las toxinas diarreicas de mariscos (DSP) tras una ingesta continuada y sus implicaciones neurológicas (CRONIC-DSP) Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2023-149618OB-I00 PAPEL DE LAS CICLOFILINAS EN LA INFLAMACION Y LA ATEROSCLEROSIS. MODULACION FARMACOLOGICA CON MOLECULAS GUIA DE ORIGEN MARINO |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Elsevier |
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Elsevier |
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reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname:Universidad de Santiago de Compostela (USC) |
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Universidad de Santiago de Compostela (USC) |
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Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
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Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
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