Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features

Nowadays, analyzing circulating tumor DNA (ctDNA), a very small part of circulating free DNA (cfDNA) carried by blood, is considered to be an interesting alternative to conventional single-site tumor tissue biopsies, both to assess tumor burden and provide a more comprehensive snapshot of the time-r...

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Detalles Bibliográficos
Autores: Campuzano Ruiz, Susana, Serafín González-Carrato, Verónica, Gamella Carballo, María, Pedrero Muñoz, María, Yáñez-Sedeño Orive, Paloma, Pingarrón Carrazón, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/12652
Acceso en línea:https://hdl.handle.net/20.500.14352/12652
Access Level:acceso abierto
Palabra clave:electrochemical biosensor
circulating tumor DNA
cancer
mutations
epigenetic changes
liquid biopsy
Química analítica (Química)
2301 Química Analítica
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spelling Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific FeaturesCampuzano Ruiz, SusanaSerafín González-Carrato, VerónicaGamella Carballo, MaríaPedrero Muñoz, MaríaYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuelelectrochemical biosensorcirculating tumor DNAcancermutationsepigenetic changesliquid biopsyQuímica analítica (Química)2301 Química AnalíticaNowadays, analyzing circulating tumor DNA (ctDNA), a very small part of circulating free DNA (cfDNA) carried by blood, is considered to be an interesting alternative to conventional single-site tumor tissue biopsies, both to assess tumor burden and provide a more comprehensive snapshot of the time-related and spatial heterogeneity of cancer genetic/epigenetic scenery. The determination of ctDNA and/or mapping its characteristic features, including tumor-specific mutations, chromosomal aberrations, microsatellite alterations, and epigenetic changes, are minimally invasive, powerful and credible biomarkers for early diagnosis, follow-up, prediction of therapy response/resistance, relapse monitoring, and tracking the rise of new mutant subclones, leading to improved cancer outcomes This review provides an outline of advances published in the last five years in electrochemical biosensing of ctDNA and surrogate markers. It emphasizes those strategies that have been successfully applied to real clinical samples. It highlights the unique opportunities they offer to shift the focus of cancer patient management methods from actual decision making, based on clinic-pathological features, to biomarker-driven treatment strategies, based on genotypes and customized targeted therapies. Also highlighted are the unmet hurdles and future key points to guide these devices in the development of liquid biopsy cornerstone tools in routine clinical practice for the diagnosis, prognosis, and therapy response monitoring in cancer patients.MDPIUniversidad Complutense de Madrid20192019-08-3020192019-08-30journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/12652reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/126522026-06-02T12:44:21Z
dc.title.none.fl_str_mv Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
title Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
spellingShingle Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
Campuzano Ruiz, Susana
electrochemical biosensor
circulating tumor DNA
cancer
mutations
epigenetic changes
liquid biopsy
Química analítica (Química)
2301 Química Analítica
title_short Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
title_full Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
title_fullStr Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
title_full_unstemmed Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
title_sort Opportunities, Challenges, and Prospects in Electrochemical Biosensing of Circulating Tumor DNA and its Specific Features
dc.creator.none.fl_str_mv Campuzano Ruiz, Susana
Serafín González-Carrato, Verónica
Gamella Carballo, María
Pedrero Muñoz, María
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author Campuzano Ruiz, Susana
author_facet Campuzano Ruiz, Susana
Serafín González-Carrato, Verónica
Gamella Carballo, María
Pedrero Muñoz, María
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author_role author
author2 Serafín González-Carrato, Verónica
Gamella Carballo, María
Pedrero Muñoz, María
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv electrochemical biosensor
circulating tumor DNA
cancer
mutations
epigenetic changes
liquid biopsy
Química analítica (Química)
2301 Química Analítica
topic electrochemical biosensor
circulating tumor DNA
cancer
mutations
epigenetic changes
liquid biopsy
Química analítica (Química)
2301 Química Analítica
description Nowadays, analyzing circulating tumor DNA (ctDNA), a very small part of circulating free DNA (cfDNA) carried by blood, is considered to be an interesting alternative to conventional single-site tumor tissue biopsies, both to assess tumor burden and provide a more comprehensive snapshot of the time-related and spatial heterogeneity of cancer genetic/epigenetic scenery. The determination of ctDNA and/or mapping its characteristic features, including tumor-specific mutations, chromosomal aberrations, microsatellite alterations, and epigenetic changes, are minimally invasive, powerful and credible biomarkers for early diagnosis, follow-up, prediction of therapy response/resistance, relapse monitoring, and tracking the rise of new mutant subclones, leading to improved cancer outcomes This review provides an outline of advances published in the last five years in electrochemical biosensing of ctDNA and surrogate markers. It emphasizes those strategies that have been successfully applied to real clinical samples. It highlights the unique opportunities they offer to shift the focus of cancer patient management methods from actual decision making, based on clinic-pathological features, to biomarker-driven treatment strategies, based on genotypes and customized targeted therapies. Also highlighted are the unmet hurdles and future key points to guide these devices in the development of liquid biopsy cornerstone tools in routine clinical practice for the diagnosis, prognosis, and therapy response monitoring in cancer patients.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-08-30
2019
2019-08-30
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/12652
url https://hdl.handle.net/20.500.14352/12652
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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