Improving genetic transformation of European chestnut and cryopreservation of transgenic lines

The aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype on the genetic transformation of embryogenic lines of European chestnut (Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an initia...

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Detalhes bibliográficos
Autores: Corredoira, Elena, San José, M. Carmen, Viéitez Martín, Ana María, Ballester, Antonio
Formato: artículo
Fecha de publicación:2007
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/48715
Acesso em linha:http://hdl.handle.net/10261/48715
Access Level:acceso abierto
Palavra-chave:Agrobacterium
Castanea sativa
Embryogenic lines
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spelling Improving genetic transformation of European chestnut and cryopreservation of transgenic linesCorredoira, ElenaSan José, M. CarmenViéitez Martín, Ana MaríaBallester, AntonioAgrobacteriumCastanea sativaEmbryogenic linesThe aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype on the genetic transformation of embryogenic lines of European chestnut (Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an initial source of explants in the transformation experiments, it was found that the use of somatic embryos isolated in the globular stage or clumps of 2-3 embryos in globular/ heart-shaped stages was more effective (30%) than when embryos at the cotyledonary stage were used (6.7%). All of the seven genotypes tested were transformed, and transformation efficiency was clearly genotype dependent. Three transgenic lines were successfully cryopreserved using the vitrification procedure, and the stable integration of the uidA gene into the transgenic chestnut plants that were regenerated subsequent to cryopreservation was demonstrated.Ministerio de Educación y Ciencia, proyecto: AGL2005-00709.Peer reviewedSpringer Nature201220122007info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/48715reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/s11240-007-9295-3info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/487152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
title Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
spellingShingle Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
Corredoira, Elena
Agrobacterium
Castanea sativa
Embryogenic lines
title_short Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
title_full Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
title_fullStr Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
title_full_unstemmed Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
title_sort Improving genetic transformation of European chestnut and cryopreservation of transgenic lines
dc.creator.none.fl_str_mv Corredoira, Elena
San José, M. Carmen
Viéitez Martín, Ana María
Ballester, Antonio
author Corredoira, Elena
author_facet Corredoira, Elena
San José, M. Carmen
Viéitez Martín, Ana María
Ballester, Antonio
author_role author
author2 San José, M. Carmen
Viéitez Martín, Ana María
Ballester, Antonio
author2_role author
author
author
dc.subject.none.fl_str_mv Agrobacterium
Castanea sativa
Embryogenic lines
topic Agrobacterium
Castanea sativa
Embryogenic lines
description The aim of the present work was to study the effect of the developmental stage of the somatic embryos and of the genotype on the genetic transformation of embryogenic lines of European chestnut (Castanea sativa Mill.) and the cryopreservation of the embryogenic lines that are generated. As an initial source of explants in the transformation experiments, it was found that the use of somatic embryos isolated in the globular stage or clumps of 2-3 embryos in globular/ heart-shaped stages was more effective (30%) than when embryos at the cotyledonary stage were used (6.7%). All of the seven genotypes tested were transformed, and transformation efficiency was clearly genotype dependent. Three transgenic lines were successfully cryopreserved using the vitrification procedure, and the stable integration of the uidA gene into the transgenic chestnut plants that were regenerated subsequent to cryopreservation was demonstrated.
publishDate 2007
dc.date.none.fl_str_mv 2007
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/48715
url http://hdl.handle.net/10261/48715
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s11240-007-9295-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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