Structure, organization and nucleotide diversity of the mitochondrial control region and cytochrome b of southern water vole (Arvicola sapidus)
The southern water vole (Arvicola sapidus Miller, 1908) is an endangered rodent whose conservation guidelines should preserve the current genetic variability. We analyze the structure and organization of the mitochondrial control region (CR) in A. sapidus. The CR of this species is characterized by...
| Autores: | , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2010 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/54709 |
| Acceso en línea: | http://hdl.handle.net/10261/54709 |
| Access Level: | acceso abierto |
| Palabra clave: | Conserved sequence blocks Cytochrome b D-loop extended termination-associated sequences Muridae Rodentia |
| Sumario: | The southern water vole (Arvicola sapidus Miller, 1908) is an endangered rodent whose conservation guidelines should preserve the current genetic variability. We analyze the structure and organization of the mitochondrial control region (CR) in A. sapidus. The CR of this species is characterized by a low guanine-cytosine content, the absence of any repetitive motif within the two hypervariable regions, and the presence of the two extended termination-associated sequences and conserved sequence blocks. Nucleotide diversity comparisons between A. sapidus and the European water vole (Arvicola terrestris) revealed differences in the distribution of genetic variation. Furthermore, we provide primers for the amplification of short and highly polymorphic fragments of CR and cytochrome b especially designed for degraded materials. These markers offer molecular tools to assist in the establishment of future conservation and management guidelines, and will also facilitate studies at different spatial and evolutionary scales of this species. |
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