Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study

Objective: To validate previously discovered miRNAs (miR-31-5p and miR-149-5p) as prognostic factors for UTUC in an independent cohort of UTUC patients.Patients and Methods: Multicenter, international and retrospective study of formalin-fixed paraffin-embedded tissue samples from 103 UTUC patients (...

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Autores: Izquierdo Reyes, Laura, Montalbo Calafell, Ruth, Ingelmo-Torres, Mercedes, Mallofré i Gómez, Carme, Ramirez-Backhaus, Miguel, Rubio, Jose, Van der Heijden, Antoine G., Schaafsma, Ewout, Lopez-Beltran, Antonio, Blanca, Ana, Lawrentschuk, Nathan, Alcaraz Asensio, Antonio, Mengual Brichs, Lourdes
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/127187
Acceso en línea:https://hdl.handle.net/2445/127187
Access Level:acceso abierto
Palabra clave:Micro RNAs
Tumors
Malalties de l'aparell urinari
MicroRNAs
Diseases of the urinary organs
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spelling Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation studyIzquierdo Reyes, LauraMontalbo Calafell, RuthIngelmo-Torres, MercedesMallofré i Gómez, CarmeRamirez-Backhaus, MiguelRubio, JoseVan der Heijden, Antoine G.Schaafsma, EwoutLopez-Beltran, AntonioBlanca, AnaLawrentschuk, NathanAlcaraz Asensio, AntonioMengual Brichs, LourdesMicro RNAsTumorsMalalties de l'aparell urinariMicroRNAsTumorsDiseases of the urinary organsObjective: To validate previously discovered miRNAs (miR-31-5p and miR-149-5p) as prognostic factors for UTUC in an independent cohort of UTUC patients.Patients and Methods: Multicenter, international and retrospective study of formalin-fixed paraffin-embedded tissue samples from 103 UTUC patients (45 progressing and 58 non-progressing) who underwent radical nephroureterectomy. Total RNA was isolated and reverse transcribed. The expression of target miRNAs (miR-31-5p and miR-149-5p) and the endogenous control miR-218-5p was evaluated in all samples by reverse transcription quantitative PCR. Normalized miRNA expression values were evaluated by multivariate forward stepwise Cox regression analysis. Kaplan Meier curves were used to discriminate between two groups of patients with a different probability of tumour progression.Results: The mean age (range) of the series was 67 (33-94) years. Overall, 45 patients (43.7%) developed tumour progression and 32 patients (31.2%) died, 20 of these (62.5%) due to their UTUC, after a median follow-up of 36 months. The mean time for tumour progression and cancer-specific survival were 15 and 20 months, respectively. Five year tumour progression free survival and cancer-specific survival were 58% for <= pT2, 36% for pT3 and 0% for pT4 and 67.8% for <= pT2, 50.6% for pT3 and 0% for pT4, respectively. In the multivariate analysis, expression of miR-31-5p was found to be an independent prognostic factor of tumour progression (HR 1.1; 95% CI 1.039-1.273; p=0.02). Kaplan Meier curve shows that miR-31-5p expression values are able to discriminate between two groups of UTUC patients with a different probability of tumour progression (p=0.007).Conclusions: We have been able to validate our previous results in an independent multicentre international cohort of UTUC patients, suggesting that miRNA-31-5p could be a useful prognostic marker of UTUC progression. The application of miRNA expression values to clinical practice could refine the currently used clinicopathological-based approach for predicting UTUC patients' outcome.Impact Journals2019201920172019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/127187Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.18632/oncotarget.17884Oncotarget, 2017, vol. 8, num. 31, p. 51522-51529https://doi.org/10.18632/oncotarget.17884cc-by (c) Izquierdo Reyes, Laura et al., 2017http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1271872026-05-29T05:05:01Z
dc.title.none.fl_str_mv Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
title Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
spellingShingle Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
Izquierdo Reyes, Laura
Micro RNAs
Tumors
Malalties de l'aparell urinari
MicroRNAs
Tumors
Diseases of the urinary organs
title_short Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
title_full Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
title_fullStr Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
title_full_unstemmed Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
title_sort Prognostic microRNAs in upper tract urothelial carcinoma: multicenter and international validation study
dc.creator.none.fl_str_mv Izquierdo Reyes, Laura
Montalbo Calafell, Ruth
Ingelmo-Torres, Mercedes
Mallofré i Gómez, Carme
Ramirez-Backhaus, Miguel
Rubio, Jose
Van der Heijden, Antoine G.
Schaafsma, Ewout
Lopez-Beltran, Antonio
Blanca, Ana
Lawrentschuk, Nathan
Alcaraz Asensio, Antonio
Mengual Brichs, Lourdes
author Izquierdo Reyes, Laura
author_facet Izquierdo Reyes, Laura
Montalbo Calafell, Ruth
Ingelmo-Torres, Mercedes
Mallofré i Gómez, Carme
Ramirez-Backhaus, Miguel
Rubio, Jose
Van der Heijden, Antoine G.
Schaafsma, Ewout
Lopez-Beltran, Antonio
Blanca, Ana
Lawrentschuk, Nathan
Alcaraz Asensio, Antonio
Mengual Brichs, Lourdes
author_role author
author2 Montalbo Calafell, Ruth
Ingelmo-Torres, Mercedes
Mallofré i Gómez, Carme
Ramirez-Backhaus, Miguel
Rubio, Jose
Van der Heijden, Antoine G.
Schaafsma, Ewout
Lopez-Beltran, Antonio
Blanca, Ana
Lawrentschuk, Nathan
Alcaraz Asensio, Antonio
Mengual Brichs, Lourdes
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Micro RNAs
Tumors
Malalties de l'aparell urinari
MicroRNAs
Tumors
Diseases of the urinary organs
topic Micro RNAs
Tumors
Malalties de l'aparell urinari
MicroRNAs
Tumors
Diseases of the urinary organs
description Objective: To validate previously discovered miRNAs (miR-31-5p and miR-149-5p) as prognostic factors for UTUC in an independent cohort of UTUC patients.Patients and Methods: Multicenter, international and retrospective study of formalin-fixed paraffin-embedded tissue samples from 103 UTUC patients (45 progressing and 58 non-progressing) who underwent radical nephroureterectomy. Total RNA was isolated and reverse transcribed. The expression of target miRNAs (miR-31-5p and miR-149-5p) and the endogenous control miR-218-5p was evaluated in all samples by reverse transcription quantitative PCR. Normalized miRNA expression values were evaluated by multivariate forward stepwise Cox regression analysis. Kaplan Meier curves were used to discriminate between two groups of patients with a different probability of tumour progression.Results: The mean age (range) of the series was 67 (33-94) years. Overall, 45 patients (43.7%) developed tumour progression and 32 patients (31.2%) died, 20 of these (62.5%) due to their UTUC, after a median follow-up of 36 months. The mean time for tumour progression and cancer-specific survival were 15 and 20 months, respectively. Five year tumour progression free survival and cancer-specific survival were 58% for <= pT2, 36% for pT3 and 0% for pT4 and 67.8% for <= pT2, 50.6% for pT3 and 0% for pT4, respectively. In the multivariate analysis, expression of miR-31-5p was found to be an independent prognostic factor of tumour progression (HR 1.1; 95% CI 1.039-1.273; p=0.02). Kaplan Meier curve shows that miR-31-5p expression values are able to discriminate between two groups of UTUC patients with a different probability of tumour progression (p=0.007).Conclusions: We have been able to validate our previous results in an independent multicentre international cohort of UTUC patients, suggesting that miRNA-31-5p could be a useful prognostic marker of UTUC progression. The application of miRNA expression values to clinical practice could refine the currently used clinicopathological-based approach for predicting UTUC patients' outcome.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/127187
url https://hdl.handle.net/2445/127187
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.18632/oncotarget.17884
Oncotarget, 2017, vol. 8, num. 31, p. 51522-51529
https://doi.org/10.18632/oncotarget.17884
dc.rights.none.fl_str_mv cc-by (c) Izquierdo Reyes, Laura et al., 2017
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Izquierdo Reyes, Laura et al., 2017
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Impact Journals
publisher.none.fl_str_mv Impact Journals
dc.source.none.fl_str_mv Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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