Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)

Spanish Cydia pomonella (L.) field populations have developed resistance to several insecticide groups. Diagnostic concentrations were established as the LC90 calculated on a susceptible strain (S_Spain) for five and seven insecticides and tested on eggs and neonate larvae field populations, respect...

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Autores: Rodríguez García, Marcela, Marques, Tânia, Bosch Serra, Dolors, Avilla Hernández, Jesús
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/46462
Acceso en línea:https://doi.org/10.1016/j.pestbp.2011.03.003
http://hdl.handle.net/10459.1/46462
Access Level:acceso abierto
Palabra clave:Eggs
Neonate larvae
Insecticide resistance
Detoxifying enzymes
Cydia pomonella
Pomera -- Malalties i plagues
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spelling Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)Rodríguez García, MarcelaMarques, TâniaBosch Serra, DolorsAvilla Hernández, JesúsEggsNeonate larvaeInsecticide resistanceDetoxifying enzymesCydia pomonellaPomera -- Malalties i plaguesSpanish Cydia pomonella (L.) field populations have developed resistance to several insecticide groups. Diagnostic concentrations were established as the LC90 calculated on a susceptible strain (S_Spain) for five and seven insecticides and tested on eggs and neonate larvae field populations, respectively. The three most relevant enzymatic detoxification systems (mixed-function oxidases (MFO), glutathione S-tranferases (GST) and esterases (EST)) were studied for neonate larvae. In eggs, 96% of the field populations showed a significantly lower efficacy when compared with the susceptible strain (S_Spain) and the most effective insecticides were fenoxycarb and thiacloprid. In neonate larvae, a significant loss of susceptibility to the insecticides was detected. Flufenoxuron, azinphos-methyl and phosmet showed the lowest efficacy, while lambda-cyhalothrin, alpha-cypermethrin and chlorpyrifos-ethyl showed the highest. Biochemical assays showed that the most important enzymatic system involved in insecticide detoxification was MFO, with highest enzymatic activity ratios (5.1–16.6 for neonates from nine field populations). An enhanced GST and EST activities was detected in one field population, with enzymatic activity ratios of threefold and fivefold for GST and EST, respectively, when compared with the susceptible strain. The insecticide bioassays showed that the LC90 used were effective as diagnostic concentrations. Measures of MFO activity alongside bioassays with insecticide diagnostic concentrations could be used as tools for monitoring insecticide resistance in neonate larvae of C. pomonella.Elsevier2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttps://doi.org/10.1016/j.pestbp.2011.03.003http://hdl.handle.net/10459.1/46462reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.pestbp.2011.03.003Pesticide Biochemistry and Physiology, 2011, vol. 100, núm. 2, p. 151-159(c) Elsevier, 2011info:eu-repo/semantics/openAccessoai:repositori.udl.cat:10459.1/464622026-06-24T12:42:17Z
dc.title.none.fl_str_mv Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
title Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
spellingShingle Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
Rodríguez García, Marcela
Eggs
Neonate larvae
Insecticide resistance
Detoxifying enzymes
Cydia pomonella
Pomera -- Malalties i plagues
title_short Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
title_full Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
title_fullStr Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
title_full_unstemmed Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
title_sort Assessment of insecticide resistance in eggs and neonate larvae of Cydia pomonella (Lepidoptera: Tortricidae)
dc.creator.none.fl_str_mv Rodríguez García, Marcela
Marques, Tânia
Bosch Serra, Dolors
Avilla Hernández, Jesús
author Rodríguez García, Marcela
author_facet Rodríguez García, Marcela
Marques, Tânia
Bosch Serra, Dolors
Avilla Hernández, Jesús
author_role author
author2 Marques, Tânia
Bosch Serra, Dolors
Avilla Hernández, Jesús
author2_role author
author
author
dc.subject.none.fl_str_mv Eggs
Neonate larvae
Insecticide resistance
Detoxifying enzymes
Cydia pomonella
Pomera -- Malalties i plagues
topic Eggs
Neonate larvae
Insecticide resistance
Detoxifying enzymes
Cydia pomonella
Pomera -- Malalties i plagues
description Spanish Cydia pomonella (L.) field populations have developed resistance to several insecticide groups. Diagnostic concentrations were established as the LC90 calculated on a susceptible strain (S_Spain) for five and seven insecticides and tested on eggs and neonate larvae field populations, respectively. The three most relevant enzymatic detoxification systems (mixed-function oxidases (MFO), glutathione S-tranferases (GST) and esterases (EST)) were studied for neonate larvae. In eggs, 96% of the field populations showed a significantly lower efficacy when compared with the susceptible strain (S_Spain) and the most effective insecticides were fenoxycarb and thiacloprid. In neonate larvae, a significant loss of susceptibility to the insecticides was detected. Flufenoxuron, azinphos-methyl and phosmet showed the lowest efficacy, while lambda-cyhalothrin, alpha-cypermethrin and chlorpyrifos-ethyl showed the highest. Biochemical assays showed that the most important enzymatic system involved in insecticide detoxification was MFO, with highest enzymatic activity ratios (5.1–16.6 for neonates from nine field populations). An enhanced GST and EST activities was detected in one field population, with enzymatic activity ratios of threefold and fivefold for GST and EST, respectively, when compared with the susceptible strain. The insecticide bioassays showed that the LC90 used were effective as diagnostic concentrations. Measures of MFO activity alongside bioassays with insecticide diagnostic concentrations could be used as tools for monitoring insecticide resistance in neonate larvae of C. pomonella.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.pestbp.2011.03.003
http://hdl.handle.net/10459.1/46462
url https://doi.org/10.1016/j.pestbp.2011.03.003
http://hdl.handle.net/10459.1/46462
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.pestbp.2011.03.003
Pesticide Biochemistry and Physiology, 2011, vol. 100, núm. 2, p. 151-159
dc.rights.none.fl_str_mv (c) Elsevier, 2011
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier, 2011
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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