Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice

Metabolic associated fatty liver disease (MAFLD) is a hepatic manifestation of metabolic syndrome and usually associated with obesity and diabetes. Our aim is to characterize the pathophysiological mechanism involved in MAFLD development in Black Tan and brachyuric (BTBR) insulin-resistant mice in c...

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Autores: Opazo Ríos, Lucas, Soto-Catalán, Manuel, Lázaro, Iolanda, Sala Vila, Aleix, Jiménez-Castilla, Luna, Orejudo, Macarena, Moreno Bravo, Juan Antonio, Egido, Jesús (Egido de los Ríos), Mas Fontao, Sebastián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/54173
Acceso en línea:http://hdl.handle.net/10230/54173
http://dx.doi.org/10.3390/ijms23073965
Access Level:acceso abierto
Palabra clave:BTBR ob/ob
De novo lipogenesis
Meta-inflammation
Metabolic associated fatty liver disease
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spelling Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob miceOpazo Ríos, LucasSoto-Catalán, ManuelLázaro, IolandaSala Vila, AleixJiménez-Castilla, LunaOrejudo, MacarenaMoreno Bravo, Juan AntonioEgido, Jesús (Egido de los Ríos)Mas Fontao, SebastiánBTBR ob/obDe novo lipogenesisMeta-inflammationMetabolic associated fatty liver diseaseMetabolic associated fatty liver disease (MAFLD) is a hepatic manifestation of metabolic syndrome and usually associated with obesity and diabetes. Our aim is to characterize the pathophysiological mechanism involved in MAFLD development in Black Tan and brachyuric (BTBR) insulin-resistant mice in combination with leptin deficiency (ob/ob). We studied liver morphology and biochemistry on our diabetic and obese mice model (BTBR ob/ob) as well as a diabetic non-obese control (BTBR + streptozotocin) and non-diabetic control mice (BTBR wild type) from 4-22 weeks. Lipid composition was assessed, and lipid related pathways were studied at transcriptional and protein level. Microvesicular steatosis was evident in BTBR ob/ob from week 6, progressing to macrovesicular in the following weeks. At 12th week, inflammatory clusters, activation of STAT3 and Nrf2 signaling pathways, and hepatocellular ballooning. At 22 weeks, the histopathological features previously observed were maintained and no signs of fibrosis were detected. Lipidomic analysis showed profiles associated with de novo lipogenesis (DNL). BTBR ob/ob mice develop MAFLD profile that resemble pathological features observed in humans, with overactivation of inflammatory response, oxidative stress and DNL signaling pathways. Therefore, BTBR ob/ob mouse is an excellent model for the study of the steatosis to steatohepatitis transition.MDPI202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/54173http://dx.doi.org/10.3390/ijms23073965reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInt J Mol Sci. 2022 Apr 2;23(7):3965© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/541732026-05-29T05:05:01Z
dc.title.none.fl_str_mv Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
title Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
spellingShingle Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
Opazo Ríos, Lucas
BTBR ob/ob
De novo lipogenesis
Meta-inflammation
Metabolic associated fatty liver disease
title_short Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
title_full Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
title_fullStr Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
title_full_unstemmed Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
title_sort Meta-inflammation and de novo lipogenesis markers are involved in metabolic associated fatty liver disease progression in BTBR ob/ob mice
dc.creator.none.fl_str_mv Opazo Ríos, Lucas
Soto-Catalán, Manuel
Lázaro, Iolanda
Sala Vila, Aleix
Jiménez-Castilla, Luna
Orejudo, Macarena
Moreno Bravo, Juan Antonio
Egido, Jesús (Egido de los Ríos)
Mas Fontao, Sebastián
author Opazo Ríos, Lucas
author_facet Opazo Ríos, Lucas
Soto-Catalán, Manuel
Lázaro, Iolanda
Sala Vila, Aleix
Jiménez-Castilla, Luna
Orejudo, Macarena
Moreno Bravo, Juan Antonio
Egido, Jesús (Egido de los Ríos)
Mas Fontao, Sebastián
author_role author
author2 Soto-Catalán, Manuel
Lázaro, Iolanda
Sala Vila, Aleix
Jiménez-Castilla, Luna
Orejudo, Macarena
Moreno Bravo, Juan Antonio
Egido, Jesús (Egido de los Ríos)
Mas Fontao, Sebastián
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BTBR ob/ob
De novo lipogenesis
Meta-inflammation
Metabolic associated fatty liver disease
topic BTBR ob/ob
De novo lipogenesis
Meta-inflammation
Metabolic associated fatty liver disease
description Metabolic associated fatty liver disease (MAFLD) is a hepatic manifestation of metabolic syndrome and usually associated with obesity and diabetes. Our aim is to characterize the pathophysiological mechanism involved in MAFLD development in Black Tan and brachyuric (BTBR) insulin-resistant mice in combination with leptin deficiency (ob/ob). We studied liver morphology and biochemistry on our diabetic and obese mice model (BTBR ob/ob) as well as a diabetic non-obese control (BTBR + streptozotocin) and non-diabetic control mice (BTBR wild type) from 4-22 weeks. Lipid composition was assessed, and lipid related pathways were studied at transcriptional and protein level. Microvesicular steatosis was evident in BTBR ob/ob from week 6, progressing to macrovesicular in the following weeks. At 12th week, inflammatory clusters, activation of STAT3 and Nrf2 signaling pathways, and hepatocellular ballooning. At 22 weeks, the histopathological features previously observed were maintained and no signs of fibrosis were detected. Lipidomic analysis showed profiles associated with de novo lipogenesis (DNL). BTBR ob/ob mice develop MAFLD profile that resemble pathological features observed in humans, with overactivation of inflammatory response, oxidative stress and DNL signaling pathways. Therefore, BTBR ob/ob mouse is an excellent model for the study of the steatosis to steatohepatitis transition.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/54173
http://dx.doi.org/10.3390/ijms23073965
url http://hdl.handle.net/10230/54173
http://dx.doi.org/10.3390/ijms23073965
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Int J Mol Sci. 2022 Apr 2;23(7):3965
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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