In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development

The Sp family of transcription factors plays important functions during development and disease. An evolutionary conserved role for some Sp family members is the control of limb development. The family is characterized by the presence of three C2H2-type zinc fingers and an adjacent 10 aa region with...

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Detalhes bibliográficos
Autores: Blom-Dahl, David, Córdoba, Sergio, Gabilondo, Hugo, Carr-Baena, Pablo, Díaz-Benjumea, Fernando J., Estella, Carlos
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/710743
Acesso em linha:http://hdl.handle.net/10486/710743
https://dx.doi.org/10.1016/j.ydbio.2020.07.011
Access Level:acceso abierto
Palavra-chave:Animals
Drosophila
Appendages
Biología y Biomedicina / Biología
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spelling In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila developmentBlom-Dahl, DavidCórdoba, SergioGabilondo, HugoCarr-Baena, PabloDíaz-Benjumea, Fernando J.Estella, CarlosAnimalsDrosophilaAppendagesBiología y Biomedicina / BiologíaThe Sp family of transcription factors plays important functions during development and disease. An evolutionary conserved role for some Sp family members is the control of limb development. The family is characterized by the presence of three C2H2-type zinc fingers and an adjacent 10 aa region with an unknown function called the Buttonhead (BTD) box. The presence of this BTD-box in all Sp family members identified from arthropods to vertebrates, suggests that it plays an essential role during development. However, despite its conservation, the in vivo function of the BTD-box has never been studied. In this work, we have generated specific BTD-box deletion alleles for the Drosophila Sp family members Sp1 and buttonhead (btd) using gene editing tools and analyzed its role during development. Unexpectedly, btd and Sp1 mutant alleles that lack the BTD-box are viable and have almost normal appendages. However, in a sensitized background the requirement of this domain to fully regulate some of Sp1 and Btd target genes is revealed. Furthermore, we have also identified a novel Sp1 role promoting leg vs antenna identity through the repression of spineless (ss) expression in the leg, a function that also depends on the Sp1 BTD-boxThis study was supported by grants from: the Programa Estatal de Generación de conocimiento y fortalecimiento científico y tecnológico del sistema de I ​+ ​D ​+ ​I(Ministerio de Ciencia, Innovación y Universidades) [No. PGC2018-095144-B-I00 to CE and BFU2014-53761-P to F.J.D.-B], the Fundación Ramón Areces and by institutional grant from Banco de Santander to the CBMSO.Elsevier Inc.Departamento de Biología MolecularFacultad de Ciencias20202020-10-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/710743https://dx.doi.org/10.1016/j.ydbio.2020.07.011reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7107432026-06-23T12:46:27Z
dc.title.none.fl_str_mv In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
title In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
spellingShingle In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
Blom-Dahl, David
Animals
Drosophila
Appendages
Biología y Biomedicina / Biología
title_short In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
title_full In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
title_fullStr In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
title_full_unstemmed In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
title_sort In vivo analysis of the evolutionary conserved BTD-box domain of Sp1 and Btd during Drosophila development
dc.creator.none.fl_str_mv Blom-Dahl, David
Córdoba, Sergio
Gabilondo, Hugo
Carr-Baena, Pablo
Díaz-Benjumea, Fernando J.
Estella, Carlos
author Blom-Dahl, David
author_facet Blom-Dahl, David
Córdoba, Sergio
Gabilondo, Hugo
Carr-Baena, Pablo
Díaz-Benjumea, Fernando J.
Estella, Carlos
author_role author
author2 Córdoba, Sergio
Gabilondo, Hugo
Carr-Baena, Pablo
Díaz-Benjumea, Fernando J.
Estella, Carlos
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Animals
Drosophila
Appendages
Biología y Biomedicina / Biología
topic Animals
Drosophila
Appendages
Biología y Biomedicina / Biología
description The Sp family of transcription factors plays important functions during development and disease. An evolutionary conserved role for some Sp family members is the control of limb development. The family is characterized by the presence of three C2H2-type zinc fingers and an adjacent 10 aa region with an unknown function called the Buttonhead (BTD) box. The presence of this BTD-box in all Sp family members identified from arthropods to vertebrates, suggests that it plays an essential role during development. However, despite its conservation, the in vivo function of the BTD-box has never been studied. In this work, we have generated specific BTD-box deletion alleles for the Drosophila Sp family members Sp1 and buttonhead (btd) using gene editing tools and analyzed its role during development. Unexpectedly, btd and Sp1 mutant alleles that lack the BTD-box are viable and have almost normal appendages. However, in a sensitized background the requirement of this domain to fully regulate some of Sp1 and Btd target genes is revealed. Furthermore, we have also identified a novel Sp1 role promoting leg vs antenna identity through the repression of spineless (ss) expression in the leg, a function that also depends on the Sp1 BTD-box
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-10-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/710743
https://dx.doi.org/10.1016/j.ydbio.2020.07.011
url http://hdl.handle.net/10486/710743
https://dx.doi.org/10.1016/j.ydbio.2020.07.011
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc.
publisher.none.fl_str_mv Elsevier Inc.
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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