Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System

[EN] Background: Cystic fibrosis (CF) is a genetic disorder that disrupts gut microbiota com position, promoting dysbiosis associated with chronic inflammation, impaired nutrient absorption, and poor clinical outcomes. While modulation of the intestinal microbiota through prebiotics, probiotics, and...

Descripción completa

Detalles Bibliográficos
Autores: Viteri-Echeverría, Jazmín Dayumara|||0000-0002-7692-9161, García Hernández, Jorge|||0000-0003-1258-6128, Heredia Gutiérrez, Ana Belén|||0000-0001-6629-9779, Andrés Grau, Ana María|||0000-0002-6132-3167, Calvo-Lerma, Joaquim, Asensio-Grau, Andrea
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/230668
Acceso en línea:https://riunet.upv.es/handle/10251/230668
Access Level:acceso abierto
Palabra clave:Beta-glucan
Dysbiosis
Colonic fermentation
Short-chain fatty acids
Bacillota/Bacteroidota
Pediatrics
id ES_8c8b2d0f307e8f4fb664ec1cbc8607fd
oai_identifier_str oai:riunet.upv.es:10251/230668
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
title Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
spellingShingle Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
Viteri-Echeverría, Jazmín Dayumara|||0000-0002-7692-9161
Beta-glucan
Dysbiosis
Colonic fermentation
Short-chain fatty acids
Bacillota/Bacteroidota
Pediatrics
title_short Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
title_full Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
title_fullStr Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
title_full_unstemmed Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
title_sort Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® System
dc.creator.none.fl_str_mv Viteri-Echeverría, Jazmín Dayumara|||0000-0002-7692-9161
García Hernández, Jorge|||0000-0003-1258-6128
Heredia Gutiérrez, Ana Belén|||0000-0001-6629-9779
Andrés Grau, Ana María|||0000-0002-6132-3167
Calvo-Lerma, Joaquim
Asensio-Grau, Andrea
author Viteri-Echeverría, Jazmín Dayumara|||0000-0002-7692-9161
author_facet Viteri-Echeverría, Jazmín Dayumara|||0000-0002-7692-9161
García Hernández, Jorge|||0000-0003-1258-6128
Heredia Gutiérrez, Ana Belén|||0000-0001-6629-9779
Andrés Grau, Ana María|||0000-0002-6132-3167
Calvo-Lerma, Joaquim
Asensio-Grau, Andrea
author_role author
author2 García Hernández, Jorge|||0000-0003-1258-6128
Heredia Gutiérrez, Ana Belén|||0000-0001-6629-9779
Andrés Grau, Ana María|||0000-0002-6132-3167
Calvo-Lerma, Joaquim
Asensio-Grau, Andrea
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Tecnología de Alimentos
Departamento de Biotecnología
Instituto Universitario de Ingeniería de Alimentos (FoodUPV)
Centro Avanzado de Microbiología Aplicada
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Ministerio de Ciencia e Innovación
UNIVERSIDAD POLITECNICA DE VALENCIA
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Beta-glucan
Dysbiosis
Colonic fermentation
Short-chain fatty acids
Bacillota/Bacteroidota
Pediatrics
topic Beta-glucan
Dysbiosis
Colonic fermentation
Short-chain fatty acids
Bacillota/Bacteroidota
Pediatrics
description [EN] Background: Cystic fibrosis (CF) is a genetic disorder that disrupts gut microbiota com position, promoting dysbiosis associated with chronic inflammation, impaired nutrient absorption, and poor clinical outcomes. While modulation of the intestinal microbiota through prebiotics, probiotics, and synbiotics has been proposed as a therapeutic strategy, clinical evidence remains limited, especially in children. Objective: This study aimed to evaluate the impact of three supplementation strategies (a prebiotic (ß-glucan), a probiotic (Lacticaseibacillus rhamnosus GG), and their synbiotic combination) on the gut microbiota and metabolic activity of a CF child faecal donor using a dynamic in vitro colonic fermenta tion model (SHIME®). Methods: Microbial composition (16S rRNA gene sequencing), and metabolic activity (quantification of short-chain fatty acids (SCFAs), ammonia, and lactate) were analysed. Results: Results showed that the prebiotic increased alpha diversity; while both the prebiotic and probiotic treatments significantly reduced Bacillota and increased Bacteroidota, modulating the Bacillota/Bacteroidota ratio. The synbiotic treatment showed the most beneficial overall profile, including enhanced production of SCFAs, particularly butyrate and propionate, and increased abundance of Faecalibacterium and Agathobacter, which are two bacterial genera generally associated with gut health. Notably, the synbiotic also reduced the relative abundance of potentially pathogenic genera such as Veillonella, Megasphaera, and Stenotrophomonas, but paralleled with an increase in Clostridium ss 1. Although the probiotic alone showed some positive effects, it was less effective overall compared to the prebiotic and synbiotic approaches. Conclusions: These findings support the potential of synbiotic supplementation as a promising strategy to modulate gut dys biosis in CF, though in vivo studies are needed to confirm the translational relevance of these results.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-11-29
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/230668
url https://riunet.upv.es/handle/10251/230668
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Universitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-06-22 ESTUDIO DE LAS INTERACCIONES FIBRA-MICROBIOTA ORIENTADO AL DESARROLLO DE SIMBIÓTICOS MODULADORES DE LA DISBIOSIS EN FIBROSIS QUÍSTICA
Universitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-01-22 Interacciones fibra-microbiota para la selección de combinaciones simbióticas con potencial corrector de la disbiosis en fibrosis quística
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 JDC2022-048768-I
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412941610090496
spelling Assessment of the Isolated and Combined Impact of beta-Glucan and Lacticaseibacillus rhamnosus on Cystic Fibrosis Gut Microbiota Using a SHIME® SystemViteri-Echeverría, Jazmín Dayumara|||0000-0002-7692-9161García Hernández, Jorge|||0000-0003-1258-6128Heredia Gutiérrez, Ana Belén|||0000-0001-6629-9779Andrés Grau, Ana María|||0000-0002-6132-3167Calvo-Lerma, JoaquimAsensio-Grau, AndreaBeta-glucanDysbiosisColonic fermentationShort-chain fatty acidsBacillota/BacteroidotaPediatrics[EN] Background: Cystic fibrosis (CF) is a genetic disorder that disrupts gut microbiota com position, promoting dysbiosis associated with chronic inflammation, impaired nutrient absorption, and poor clinical outcomes. While modulation of the intestinal microbiota through prebiotics, probiotics, and synbiotics has been proposed as a therapeutic strategy, clinical evidence remains limited, especially in children. Objective: This study aimed to evaluate the impact of three supplementation strategies (a prebiotic (ß-glucan), a probiotic (Lacticaseibacillus rhamnosus GG), and their synbiotic combination) on the gut microbiota and metabolic activity of a CF child faecal donor using a dynamic in vitro colonic fermenta tion model (SHIME®). Methods: Microbial composition (16S rRNA gene sequencing), and metabolic activity (quantification of short-chain fatty acids (SCFAs), ammonia, and lactate) were analysed. Results: Results showed that the prebiotic increased alpha diversity; while both the prebiotic and probiotic treatments significantly reduced Bacillota and increased Bacteroidota, modulating the Bacillota/Bacteroidota ratio. The synbiotic treatment showed the most beneficial overall profile, including enhanced production of SCFAs, particularly butyrate and propionate, and increased abundance of Faecalibacterium and Agathobacter, which are two bacterial genera generally associated with gut health. Notably, the synbiotic also reduced the relative abundance of potentially pathogenic genera such as Veillonella, Megasphaera, and Stenotrophomonas, but paralleled with an increase in Clostridium ss 1. Although the probiotic alone showed some positive effects, it was less effective overall compared to the prebiotic and synbiotic approaches. Conclusions: These findings support the potential of synbiotic supplementation as a promising strategy to modulate gut dys biosis in CF, though in vivo studies are needed to confirm the translational relevance of these results. This work was funded by the Universitat Politècnica de València within the framework of the grant awarded to Andrea Asensio-Grau (Grants for First Research Projects 2022, ref. PAID-06-22). The authors also thank the Universitat Politècnica de València for the support granted to Jazmín Viteri-Echeverría [Predoctoral Contract Grants-SUBPR 1, ref. PAID-01-22]. Andrea Asensio-Grau acknowledges the Juan de la Cierva postdoctoral research grant awarded by the Spanish Ministry of Science and Innovation (JDC2022-048768-I).MDPI AGDepartamento de Tecnología de AlimentosDepartamento de BiotecnologíaInstituto Universitario de Ingeniería de Alimentos (FoodUPV)Centro Avanzado de Microbiología AplicadaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalMinisterio de Ciencia e InnovaciónUNIVERSIDAD POLITECNICA DE VALENCIARepositorio Institucional de la Universitat Politècnica de València Riunet20252025-11-29journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/230668reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengUniversitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-06-22 ESTUDIO DE LAS INTERACCIONES FIBRA-MICROBIOTA ORIENTADO AL DESARROLLO DE SIMBIÓTICOS MODULADORES DE LA DISBIOSIS EN FIBROSIS QUÍSTICAUniversitat Politècnica de València https://doi.org/10.13039/501100004233 PAID-01-22 Interacciones fibra-microbiota para la selección de combinaciones simbióticas con potencial corrector de la disbiosis en fibrosis quísticaMinisterio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 JDC2022-048768-Iopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2306682026-06-13T07:49:27Z
score 15,811543

Similares en LA Referencia