Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach

[Data availability] Data supporting the findings of this study are openly available in the Gene Expression Omnibus repository under reference number GSE144344.

Detalles Bibliográficos
Autores: Madloo, Pari, Lema, Margarita, Cartea, Maria Elena, Soengas, Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/415017
Acceso en línea:http://hdl.handle.net/10261/415017
https://api.elsevier.com/content/abstract/scopus_id/85115904105
Access Level:acceso abierto
Palabra clave:Brassica
Indoles
Isothiocyanates
Plant secondary metabolites
Plant-pathogen interaction
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spelling Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic ApproachMadloo, PariLema, MargaritaCartea, Maria ElenaSoengas, PilarBrassicaIndolesIsothiocyanatesPlant secondary metabolitesPlant-pathogen interaction[Data availability] Data supporting the findings of this study are openly available in the Gene Expression Omnibus repository under reference number GSE144344.White mold disease, caused by the necrotrophic fungus Sclerotinia sclerotiorum, affects Brassica crops. Brassica crops produce a broad array of compounds, such as glucosinolates, which contribute to the defense against pathogens. From their hydrolysis, several products arise that have antimicrobial activity (GHPs) whose toxicity is structure dependent. S. sclerotiorum may overcome the toxic effect of moderate GHP concentrations after prolonged exposure to their action. Our objective was to identify the molecular mechanism underlying S. sclerotiorum response to long exposure to two chemically diverse GHPs: aliphatic GHP allyl-isothiocyanate (AITC) and indole GHP indol-3-carbinol (I3C). We found that the transcriptomic response is dependent on the type of GHP and on their initial target, involving cell membranes in the case of AITC or DNA in the case of I3C. Response mechanisms include the reorganization of chromatin, mediated by histone chaperones hip4 and cia1, ribosome synthesis controlled by the kinase-phosphatase pair aps1-ppn1, catabolism of proteins, ergosterol synthesis, and induction of detoxification systems. These mechanisms probably help S. sclerotiorum to grow and survive in an environment where GHPs are constantly produced by Brassica plants upon glucosinolate breakdown. IMPORTANCEBrassica species, including important vegetable crops, such as cabbage, cauliflower, or broccoli, or oil crops, such as rapeseed, produce specific chemical compounds useful to protect them against pests and pathogens. One of the most destructive Brassica diseases in temperate areas around the world is Sclerotinia stem rot, caused by the fungus Sclerotinia sclerotiorum. This is a generalist pathogen that causes disease over more than 400 plant species, being a serious threat to economically important crops worldwide, including potato, bean, soybean, and sunflower, among many others. Understanding the mechanisms utilized by pathogens to overcome specific plant defensive compounds can be useful to increase plant resistance. Our study demonstrated that Sclerotinia shows different adaptation mechanisms, including detoxification systems, to grow and survive when plant protective compounds are present.This research was supported by projects AGL2015-66256-C2-R and RTI2018-096591-B-I00 through the Spanish Ministry of Science and the European Regional Development Fund (ERDF). Pari Madloo acknowledges a Ph.D. student fellowship from Erasmus Mundus Action 2 Program. P.M. did the experimental work. M.L. and P.S. designed the experiments. P.M., M.L., M.E.C., and P.S. contributed to the discussion of results. P.M. and P.S. wrote the manuscript. All authors reviewed the manuscript. We declare no conflicts of interest.Peer reviewedAmerican Society for MicrobiologyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/415017https://api.elsevier.com/content/abstract/scopus_id/85115904105reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1128/spectrum.00180-21https://doi.org/10.1128/spectrum.00180-21Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4150172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
title Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
spellingShingle Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
Madloo, Pari
Brassica
Indoles
Isothiocyanates
Plant secondary metabolites
Plant-pathogen interaction
title_short Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
title_full Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
title_fullStr Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
title_full_unstemmed Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
title_sort Sclerotinia sclerotiorum Response to Long Exposure to Glucosinolate Hydrolysis Products by Transcriptomic Approach
dc.creator.none.fl_str_mv Madloo, Pari
Lema, Margarita
Cartea, Maria Elena
Soengas, Pilar
author Madloo, Pari
author_facet Madloo, Pari
Lema, Margarita
Cartea, Maria Elena
Soengas, Pilar
author_role author
author2 Lema, Margarita
Cartea, Maria Elena
Soengas, Pilar
author2_role author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Brassica
Indoles
Isothiocyanates
Plant secondary metabolites
Plant-pathogen interaction
topic Brassica
Indoles
Isothiocyanates
Plant secondary metabolites
Plant-pathogen interaction
description [Data availability] Data supporting the findings of this study are openly available in the Gene Expression Omnibus repository under reference number GSE144344.
publishDate 2021
dc.date.none.fl_str_mv 2021
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/415017
https://api.elsevier.com/content/abstract/scopus_id/85115904105
url http://hdl.handle.net/10261/415017
https://api.elsevier.com/content/abstract/scopus_id/85115904105
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1128/spectrum.00180-21
https://doi.org/10.1128/spectrum.00180-21
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412935750647808
score 15,81155

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