Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells

Surface biophysical properties of biomaterials, including surface topography and roughness, determine the interaction of a biomaterial with the surrounding cells, tissues and organs once implanted in the human body. Herein, the surface topography of thermoplastic copolymers based on lactide and capr...

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Autores: Bello Álvarez, Carlos, Atxa Ainz, Blanca, Muñoz Ugartemendia, Jone, Sebastián, Laura, Etxeberria Lizarraga, Agustín, Sarasua Oiz, José Ramón, Zuza Hernández, Ester, Larrañaga Espartero, Aitor
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/63674
Acceso en línea:http://hdl.handle.net/10810/63674
Access Level:acceso abierto
Palabra clave:thermoplastic elastomers
chain microstructure
lactide
surface topography
cell interaction
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spelling Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cellsBello Álvarez, CarlosAtxa Ainz, BlancaMuñoz Ugartemendia, JoneSebastián, LauraEtxeberria Lizarraga, AgustínSarasua Oiz, José RamónZuza Hernández, EsterLarrañaga Espartero, Aitorthermoplastic elastomerschain microstructurelactidesurface topographycell interactionSurface biophysical properties of biomaterials, including surface topography and roughness, determine the interaction of a biomaterial with the surrounding cells, tissues and organs once implanted in the human body. Herein, the surface topography of thermoplastic copolymers based on lactide and caprolactone showing elastomeric behaviour was modulated by precisely controlling the chain microstructure (i.e., distribution and length of the repetitive units within the polymeric chain). The synthesized copolymers were subjected to different thermal treatments from the melt, leading to polymeric films with various surface textures and roughness values. Copolymers synthesized with triphenyl bismuth as a catalyst, with a more random distribution of the repetitive units, showed limited crystallization capability. Accordingly, only the copolymer with higher amount of l-lactide (i.e., 80 wt%) subjected to an isothermal treatment from the melt at 70 °C was able to crystallize, and spherulites of around 7 μm were discernible by atomic force microscopy. In contrast, the copolymers synthesized with stannous octoate, which had a more blocky nature, showed axialitic crystalline domains at the submicron-to nanoscale when subjected to an isothermal treatment from the melt at 50 °C, whereas well-defined spherulites of sizes up to 14 μm were obtained at 70 °C. Human fibroblast showed a more elongated morphology when seeded on those samples having higher roughness values and larger spherulites, whereas they had a more spread morphology when seeded on the amorphous, smooth surfaces. As concluded from the present study, by precisely controlling the chain microstructure of the synthesized copolymers, a wide variety of surface topographies can be obtained, which has a clear impact on the way the biomaterial interacts with cells. This opens the possibility to study the influence of surface biophysical properties on more complex cell processes in the future, including inflammatory or foreign body response processes.This work has been funded by the Basque Government (GV/EJ) - Department of Education (GIC21/131 IT1766-22), grant PID2019-106236 GB-I00 funded by MCIN/AEI/10.13039/501100011033 and PID2022-139821OB-I00 funded by MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe”. C.B-Á. acknowledges the predoctoral grant funded by the UPV/EHU.Elsevier202320232023info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/63674reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICINN/PID2019-106236 GB-I00/info:eu-repo/grantAgreement/MICINN/PID2022-139821OB-I00/https://www.sciencedirect.com/science/article/pii/S0142941823003008info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).Atribución 3.0 Españaoai:addi.ehu.eus:10810/636742026-06-18T09:23:17Z
dc.title.none.fl_str_mv Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
title Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
spellingShingle Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
Bello Álvarez, Carlos
thermoplastic elastomers
chain microstructure
lactide
surface topography
cell interaction
title_short Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
title_full Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
title_fullStr Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
title_full_unstemmed Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
title_sort Thermoplastic elastomers based on lactide and caprolactone: The influence of chain microstructure on surface topography and subsequent interaction with cells
dc.creator.none.fl_str_mv Bello Álvarez, Carlos
Atxa Ainz, Blanca
Muñoz Ugartemendia, Jone
Sebastián, Laura
Etxeberria Lizarraga, Agustín
Sarasua Oiz, José Ramón
Zuza Hernández, Ester
Larrañaga Espartero, Aitor
author Bello Álvarez, Carlos
author_facet Bello Álvarez, Carlos
Atxa Ainz, Blanca
Muñoz Ugartemendia, Jone
Sebastián, Laura
Etxeberria Lizarraga, Agustín
Sarasua Oiz, José Ramón
Zuza Hernández, Ester
Larrañaga Espartero, Aitor
author_role author
author2 Atxa Ainz, Blanca
Muñoz Ugartemendia, Jone
Sebastián, Laura
Etxeberria Lizarraga, Agustín
Sarasua Oiz, José Ramón
Zuza Hernández, Ester
Larrañaga Espartero, Aitor
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv thermoplastic elastomers
chain microstructure
lactide
surface topography
cell interaction
topic thermoplastic elastomers
chain microstructure
lactide
surface topography
cell interaction
description Surface biophysical properties of biomaterials, including surface topography and roughness, determine the interaction of a biomaterial with the surrounding cells, tissues and organs once implanted in the human body. Herein, the surface topography of thermoplastic copolymers based on lactide and caprolactone showing elastomeric behaviour was modulated by precisely controlling the chain microstructure (i.e., distribution and length of the repetitive units within the polymeric chain). The synthesized copolymers were subjected to different thermal treatments from the melt, leading to polymeric films with various surface textures and roughness values. Copolymers synthesized with triphenyl bismuth as a catalyst, with a more random distribution of the repetitive units, showed limited crystallization capability. Accordingly, only the copolymer with higher amount of l-lactide (i.e., 80 wt%) subjected to an isothermal treatment from the melt at 70 °C was able to crystallize, and spherulites of around 7 μm were discernible by atomic force microscopy. In contrast, the copolymers synthesized with stannous octoate, which had a more blocky nature, showed axialitic crystalline domains at the submicron-to nanoscale when subjected to an isothermal treatment from the melt at 50 °C, whereas well-defined spherulites of sizes up to 14 μm were obtained at 70 °C. Human fibroblast showed a more elongated morphology when seeded on those samples having higher roughness values and larger spherulites, whereas they had a more spread morphology when seeded on the amorphous, smooth surfaces. As concluded from the present study, by precisely controlling the chain microstructure of the synthesized copolymers, a wide variety of surface topographies can be obtained, which has a clear impact on the way the biomaterial interacts with cells. This opens the possibility to study the influence of surface biophysical properties on more complex cell processes in the future, including inflammatory or foreign body response processes.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/63674
url http://hdl.handle.net/10810/63674
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/PID2019-106236 GB-I00/
info:eu-repo/grantAgreement/MICINN/PID2022-139821OB-I00/
https://www.sciencedirect.com/science/article/pii/S0142941823003008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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