Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly

333 p.

Detalhes bibliográficos
Autor: Pérez Rodríguez, Aitzol
Formato: tesis doctoral
Fecha de publicación:2024
País:España
Recursos:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/72607
Acesso em linha:http://hdl.handle.net/10810/72607
Access Level:acceso embargado
Palavra-chave:alkaloids
peptides
mycology
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oai_identifier_str oai:addi.ehu.eus:10810/72607
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
Erregina-jeleko jeleina-II peptido antimikrobianoaren Candidaren aurkako jarduera antifungikoaren hobekuntza
title Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
spellingShingle Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
Pérez Rodríguez, Aitzol
alkaloids
peptides
mycology
title_short Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
title_full Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
title_fullStr Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
title_full_unstemmed Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
title_sort Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jelly
dc.creator.none.fl_str_mv Pérez Rodríguez, Aitzol
author Pérez Rodríguez, Aitzol
author_facet Pérez Rodríguez, Aitzol
author_role author
dc.contributor.none.fl_str_mv Mateo Alesanco, Estibaliz
Eraso Barrio, María Elena
dc.subject.none.fl_str_mv alkaloids
peptides
mycology
topic alkaloids
peptides
mycology
description 333 p.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2025
2025
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/72607
url http://hdl.handle.net/10810/72607
dc.language.none.fl_str_mv Inglés
Euskera
language_invalid_str_mv Inglés
Euskera
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
© 2024 Aitzol Pérez Rodríguez
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv © 2024 Aitzol Pérez Rodríguez
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412877625982976
spelling Enhancement of the antifungal activity against Candida of jelleine-II, an antimicrobial peptide from the royal jellyErregina-jeleko jeleina-II peptido antimikrobianoaren Candidaren aurkako jarduera antifungikoaren hobekuntzaPérez Rodríguez, Aitzolalkaloidspeptidesmycology333 p.EN: Candidiasis, the most frequent opportunistic fungal infections, can range from a superficial diseaseto a life-threatening invasive candidiasis. Candida albicans remains the predominant aetiological agent,but the incidence of candidiasis caused by Candida species with reduced susceptibility to some of thecommonly employed antifungal drugs is increasing. Therefore, there is an urgent need to find novelantifungal compounds to face these resistant pathogens. The jelleines are a family of four shortantimicrobial peptides, present in the royal jelly of the bee Apis mellifera, that have been reported topossess antimicrobial activity against gram-positive and gram-negative bacteria, as well as againstCandida. The jelleine-I peptide has hoarded the focus among the four peptides, but the jelleine-II showeda comparable antimicrobial effect in their initial characterisation.Therefore, this Thesis work consisted in the characterisation and evaluation of the antifungal efficacy ofthe jelleine-II peptide and some derivatives against Candida species, including C. albicans, Candidaglabrata, Candida parapsilosis, Candida krusei and Candida auris. The sequence of the wild-typejelleine-II was altered by amino acid substitutions by following various approaches. Alanine scanningwas performed to determine the importance of each amino acid in the sequence of the jelleine-II for theantifungal activity of the peptide. Derivatives with arginine and/or tryptophan residues were tested withthe aim to obtain a derivative with enhanced antifungal activity against the aforementioned Candidaspecies.The evaluation of the antifungal efficacy via in vitro and in vivo assays led to the selection of a derivativeof the wild-type jelleine-II peptide, the JII-R1 peptide, which showed an improved antifungal activity andsafety profile in comparison to the parent peptide. The jelleine-II peptide and some of its derivativesexerted antifungal activity against both planktonic and sessile cells of Candida. As a conclusion, thosepeptides, especially the JII-R1 derivative, are promising antifungal molecules because of their lowtoxicity and activity against Candida species.EUS: Kandidiasiak, infekzio fungiko oportunista ohikoenetarikoak direnak, azaleko gaixotasun batetikbizia arriskuan jartzen duen kandidiasi inbaditzaile batera bitartekoak izan daitezke. Candida albicans daeragile etiologiko nagusia, baina erabili ohi diren sendagai antifungikoekiko sentikortasun murriztuakdauzkaten Candida espezieek eragindako kandidiasien intzidentzia handitzen ari da. Beraz, patogenoerresistente horiei aurre egiteko konposatu antifungiko berriak aurkitzea premiazkoa da. Jeleinak laupeptido antimikrobiano txikien familia bat dira, Apis mellifera erlearen erregina-jelean aurkitzen direnak.Jakinarazi denez, jeleinek bakterio gram-positibo zein gram-negatiboen eta Candidaren aurkako jardueraantimikrobiano dute. Jeleina-I peptidoa izan da lau peptidoen artean fokua bereganatu duena, bainajeleina-II peptidoak jarduera antimikrobiano konparagarria aurkeztu izan zuen haien lehendeskribapenean.Horregatik, Tesi lan hau jeleina-II peptidoaren eta haren deribatu batzuen Candidaren aurkako jardueraantifungikoaren karakterizazioan eta ebaluazioan oinarritu egin zen, C. albicans, Candida glabrata,Candida parapsilosis, Candida krusei eta Candida auris espezieen aurkako efektuak aztertuz. Jeleina-IIpeptidoaren sekuentzian aminoazidoen ordezkapena burutu egin zen, hainbat hurbilketa desberdinjarraituz. ¿Alanine scanning¿ teknikaren bitartez aminoazido bakoitzak jeleina-II peptidoaren sekuentzianjarduera antifungikorako duen garrantzia aztertu egin zen. Arginina eta/edo triptofano aminoazidoekindiseinatutako deribatuak aztertu egin ziren aurretik aipaturiko Candida espezieen aurkako jardueraantifungiko indartua zuen deribatu bat lortzeko helburuarekin.In vitro eta in vivo azterlanen bidezko jarduera antifungikoaren ebaluazioak jeleina-II peptidotikeratorritako deribatu bat, JII-R1 deribatua, hautatzea ekarri zuen, zeinak peptido originalarekinalderaturik jarduera antifungiko eta segurtasun profil hobeak erakutsi zituen. Jeleina-II peptidoak etaharen deribatu batzuek Candida zelula planktoniko eta sesilen aurkako jarduera antifungikoa erakutsizuten. Ondorio gisa, peptido hauek, JII-R1 deribatua bereziki, etorkizun handiko molekula antifungikoakdira haien toxizitate baxuagatik eta Candida espezieetan duten jarduera antifungikoagatikMateo Alesanco, EstibalizEraso Barrio, María Elena20262025202520242024info:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfhttp://hdl.handle.net/10810/72607reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésEuskerainfo:eu-repo/semantics/embargoedAccess© 2024 Aitzol Pérez Rodríguezoai:addi.ehu.eus:10810/726072026-06-18T09:23:17Z
score 15,198674

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