The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes

Adipogenesis is a tightly regulated process, and the involvement of autophagy has been recently proposed in mammalian models. In rainbow trout, two well-defined phases describe the development of primary cultured adipocyte cells: proliferation and differentiation. Nevertheless, information on the tr...

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Authors: Riera Heredia, Natàlia, Lutfi Royo, Esmail, Balbuena-Pecino, Sara, Vélez, Emilio J., Dias, Karine, Beaumatin, Florian, Gutiérrez Fruitós, Joaquín, Seiliez, Iban, Capilla Campos, Encarnación, Navarro Álvarez, Isabel
Format: article
Status:Published version
Publication Date:2022
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/182850
Online Access:https://hdl.handle.net/2445/182850
Access Level:Open access
Keyword:Peix
Autofàgia
Fish as food
Autophagy
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spelling The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytesRiera Heredia, NatàliaLutfi Royo, EsmailBalbuena-Pecino, SaraVélez, Emilio J.Dias, KarineBeaumatin, FlorianGutiérrez Fruitós, JoaquínSeiliez, IbanCapilla Campos, EncarnaciónNavarro Álvarez, IsabelPeixAutofàgiaFish as foodAutophagyAdipogenesis is a tightly regulated process, and the involvement of autophagy has been recently proposed in mammalian models. In rainbow trout, two well-defined phases describe the development of primary cultured adipocyte cells: proliferation and differentiation. Nevertheless, information on the transcriptional profile at the onset of differentiation and the potential role of autophagy in this process is scarce. In the present study, the cells showed an early and transient induction of several adipogenic transcription factors genes' expression (i.e., cebpa and cebpb) along with the morphological changes (round shape filled with small lipid droplets) typical of the onset of adipogenesis. Then, the expression of various lipid metabolism-related genes involving the synthesis (fas), uptake (fatp1 and cd36), accumulation (plin2) and mobilization (hsl) of lipids, characteristic of the mature adipocyte, increased. In parallel, several autophagy markers (i.e., atg4b, gabarapl1 and lc3b) mirrored the expression of those adipogenic-related genes, suggesting a role of autophagy during in vitro fish adipogenesis. In this regard, the incubation of preadipocytes with lysosomal inhibitors (Bafilomycin A1 or Chloroquine), described to prevent autophagy flux, delayed the process of adipogenesis (i.e., cell remodelling), thus suggesting a possible relationship between autophagy and adipocyte differentiation in trout. Moreover, the disruption of the autophagic flux altered the expression of some key adipogenic genes such as cebpa and pparg. Overall, this study contributes to improve our knowledge on the regulation of rainbow trout adipocyte differentiation, and highlights for the first time in fish the involvement of autophagy on adipogenesis, suggesting a close-fitting connection between both processes.Elsevier Ltd2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/182850Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.cbpb.2021.110700Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2022, vol. 258https://doi.org/10.1016/j.cbpb.2021.110700cc-by (c) Riera-Heredia, Natàlia et al., 2022https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1828502026-05-29T05:05:01Z
dc.title.none.fl_str_mv The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
title The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
spellingShingle The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
Riera Heredia, Natàlia
Peix
Autofàgia
Fish as food
Autophagy
title_short The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
title_full The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
title_fullStr The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
title_full_unstemmed The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
title_sort The autophagy response during adipogenesis of primary cultured rainbow trout (Oncorhynchus mykiss) adipocytes
dc.creator.none.fl_str_mv Riera Heredia, Natàlia
Lutfi Royo, Esmail
Balbuena-Pecino, Sara
Vélez, Emilio J.
Dias, Karine
Beaumatin, Florian
Gutiérrez Fruitós, Joaquín
Seiliez, Iban
Capilla Campos, Encarnación
Navarro Álvarez, Isabel
author Riera Heredia, Natàlia
author_facet Riera Heredia, Natàlia
Lutfi Royo, Esmail
Balbuena-Pecino, Sara
Vélez, Emilio J.
Dias, Karine
Beaumatin, Florian
Gutiérrez Fruitós, Joaquín
Seiliez, Iban
Capilla Campos, Encarnación
Navarro Álvarez, Isabel
author_role author
author2 Lutfi Royo, Esmail
Balbuena-Pecino, Sara
Vélez, Emilio J.
Dias, Karine
Beaumatin, Florian
Gutiérrez Fruitós, Joaquín
Seiliez, Iban
Capilla Campos, Encarnación
Navarro Álvarez, Isabel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Peix
Autofàgia
Fish as food
Autophagy
topic Peix
Autofàgia
Fish as food
Autophagy
description Adipogenesis is a tightly regulated process, and the involvement of autophagy has been recently proposed in mammalian models. In rainbow trout, two well-defined phases describe the development of primary cultured adipocyte cells: proliferation and differentiation. Nevertheless, information on the transcriptional profile at the onset of differentiation and the potential role of autophagy in this process is scarce. In the present study, the cells showed an early and transient induction of several adipogenic transcription factors genes' expression (i.e., cebpa and cebpb) along with the morphological changes (round shape filled with small lipid droplets) typical of the onset of adipogenesis. Then, the expression of various lipid metabolism-related genes involving the synthesis (fas), uptake (fatp1 and cd36), accumulation (plin2) and mobilization (hsl) of lipids, characteristic of the mature adipocyte, increased. In parallel, several autophagy markers (i.e., atg4b, gabarapl1 and lc3b) mirrored the expression of those adipogenic-related genes, suggesting a role of autophagy during in vitro fish adipogenesis. In this regard, the incubation of preadipocytes with lysosomal inhibitors (Bafilomycin A1 or Chloroquine), described to prevent autophagy flux, delayed the process of adipogenesis (i.e., cell remodelling), thus suggesting a possible relationship between autophagy and adipocyte differentiation in trout. Moreover, the disruption of the autophagic flux altered the expression of some key adipogenic genes such as cebpa and pparg. Overall, this study contributes to improve our knowledge on the regulation of rainbow trout adipocyte differentiation, and highlights for the first time in fish the involvement of autophagy on adipogenesis, suggesting a close-fitting connection between both processes.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/182850
url https://hdl.handle.net/2445/182850
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.cbpb.2021.110700
Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2022, vol. 258
https://doi.org/10.1016/j.cbpb.2021.110700
dc.rights.none.fl_str_mv cc-by (c) Riera-Heredia, Natàlia et al., 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Riera-Heredia, Natàlia et al., 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412876550144000
score 15,811543

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