Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer

[EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multi-disciplinary approach using confocal and electron microscopy, detect...

Full description

Bibliographic Details
Authors: Rivas-Sendra, Alba, Corral Martínez, Patricia, Camacho-Fernández, Carolina, Calabuig-Serna, Antonio, Porcel, R.|||0000-0001-8068-741X, Seguí-Simarro, Jose M.|||0000-0001-7672-4169
Format: article
Publication Date:2019
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:riunet.upv.es:10251/155497
Online Access:https://riunet.upv.es/handle/10251/155497
Access Level:Open access
Keyword:Androgenesis
Brassica napus
Calcium
Cellulose
Cell wall
Doubled haploids
Eggplant
Rapeseed
GENETICA
BIOQUIMICA Y BIOLOGIA MOLECULAR
id ES_8bd0b2da970b2ffe88396d38f4a96fef
oai_identifier_str oai:riunet.upv.es:10251/155497
network_acronym_str ES
network_name_str España
repository_id_str
spelling Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layerRivas-Sendra, AlbaCorral Martínez, PatriciaCamacho-Fernández, CarolinaCalabuig-Serna, AntonioPorcel, R.|||0000-0001-8068-741XSeguí-Simarro, Jose M.|||0000-0001-7672-4169AndrogenesisBrassica napusCalciumCelluloseCell wallDoubled haploidsEggplantRapeseedGENETICABIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multi-disciplinary approach using confocal and electron microscopy, detection of Ca2+, callose, and cellulose, treatments with caffeine, digitonin, and endosidin7, morphometry, qPCR, osmometry, and viability assays in order to study the dynamics of cell wall formation during embryogenesis induction in a high-response rapeseed (Brassica napus) line and two recalcitrant rapeseed and eggplant (Solanum melongena) lines. Formation of a callose-rich subintinal layer (SL) was common to microspore embryogenesis in the different genotypes. However, this process was directly related to embryogenic response, being greater in high-response genotypes. A link could be established between Ca2+ influx, abnormal callose/cellulose deposition, and the genotype-specific embryogenic competence. Callose deposition in inner walls and SLs are independent processes, regulated by different callose synthases. Viability and control of internal osmolality are also related to SL formation. In summary, we identified one of the causes of recalcitrance to embryogenesis induction: a reduced or absent protective SL. In responding genotypes, SLs are markers for changes in cell fate and serve as osmoprotective barriers to increase viability in imbalanced in vitro environments. Genotype-specific differences relate to different responses against abiotic (heat/osmotic) stresses.Thanks are due to the Electron Microscopy Service of Universitat Politecnica de Valencia, Marisol Gascon (IBMCP Microscopy Service), Dr Kim Boutilier (WUR, Wageningen) for hosting ARS at her lab, and Dr Samantha Vernhettes (INRA Versailles) for kindly providing us with S4B. This work supported by grants AGL2014-55177-R and AGL2017-88135-R to JMSS from MINECO jointly funded by FEDER.Oxford University PressInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalEuropean Regional Development FundMinisterio de Economía y CompetitividadAgencia Estatal de InvestigaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20192019-02-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/155497reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2014-55177-R NUEVAS VIAS DE MEJORA DE LA EMBRIOGENESIS DE MICROSPORAS EN SOLANACEAS RECALCITRANTES: ESTUDIO DE LA AUTOFAGIA, LA UPR Y LA REGULACION HORMONALAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 AGL2017-88135-R DISECCION DE LA RESPUESTA EMBRIOGENICA DE LAS MICROSPORAS: ANALISIS FISIOLOGICO Y GENOMICO DE LA RECALCITRANCIA A LA INDUCCION DE EMBRIOGENESISopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1554972026-06-13T07:49:27Z
dc.title.none.fl_str_mv Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
title Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
spellingShingle Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
Rivas-Sendra, Alba
Androgenesis
Brassica napus
Calcium
Cellulose
Cell wall
Doubled haploids
Eggplant
Rapeseed
GENETICA
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
title_full Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
title_fullStr Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
title_full_unstemmed Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
title_sort Embryogenic competence of microspores is associated to their ability to form a callosic, osmoprotective subintinal layer
dc.creator.none.fl_str_mv Rivas-Sendra, Alba
Corral Martínez, Patricia
Camacho-Fernández, Carolina
Calabuig-Serna, Antonio
Porcel, R.|||0000-0001-8068-741X
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author Rivas-Sendra, Alba
author_facet Rivas-Sendra, Alba
Corral Martínez, Patricia
Camacho-Fernández, Carolina
Calabuig-Serna, Antonio
Porcel, R.|||0000-0001-8068-741X
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author_role author
author2 Corral Martínez, Patricia
Camacho-Fernández, Carolina
Calabuig-Serna, Antonio
Porcel, R.|||0000-0001-8068-741X
Seguí-Simarro, Jose M.|||0000-0001-7672-4169
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
European Regional Development Fund
Ministerio de Economía y Competitividad
Agencia Estatal de Investigación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Androgenesis
Brassica napus
Calcium
Cellulose
Cell wall
Doubled haploids
Eggplant
Rapeseed
GENETICA
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic Androgenesis
Brassica napus
Calcium
Cellulose
Cell wall
Doubled haploids
Eggplant
Rapeseed
GENETICA
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multi-disciplinary approach using confocal and electron microscopy, detection of Ca2+, callose, and cellulose, treatments with caffeine, digitonin, and endosidin7, morphometry, qPCR, osmometry, and viability assays in order to study the dynamics of cell wall formation during embryogenesis induction in a high-response rapeseed (Brassica napus) line and two recalcitrant rapeseed and eggplant (Solanum melongena) lines. Formation of a callose-rich subintinal layer (SL) was common to microspore embryogenesis in the different genotypes. However, this process was directly related to embryogenic response, being greater in high-response genotypes. A link could be established between Ca2+ influx, abnormal callose/cellulose deposition, and the genotype-specific embryogenic competence. Callose deposition in inner walls and SLs are independent processes, regulated by different callose synthases. Viability and control of internal osmolality are also related to SL formation. In summary, we identified one of the causes of recalcitrance to embryogenesis induction: a reduced or absent protective SL. In responding genotypes, SLs are markers for changes in cell fate and serve as osmoprotective barriers to increase viability in imbalanced in vitro environments. Genotype-specific differences relate to different responses against abiotic (heat/osmotic) stresses.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-02-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/155497
url https://riunet.upv.es/handle/10251/155497
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 AGL2014-55177-R NUEVAS VIAS DE MEJORA DE LA EMBRIOGENESIS DE MICROSPORAS EN SOLANACEAS RECALCITRANTES: ESTUDIO DE LA AUTOFAGIA, LA UPR Y LA REGULACION HORMONAL
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 AGL2017-88135-R DISECCION DE LA RESPUESTA EMBRIOGENICA DE LAS MICROSPORAS: ANALISIS FISIOLOGICO Y GENOMICO DE LA RECALCITRANCIA A LA INDUCCION DE EMBRIOGENESIS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412853463646208
score 15,301603

Similar Items