A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus

P1 is the first protein translated from the genomes of most viruses in the family Potyviridae, and it contains a C-terminal serine-protease domain that cis-cleaves the junction between P1 and HCPro in most cases. Intriguingly, P1 is the most divergent among all mature viral factors, and its roles du...

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Autores: Gou, Bei, Dai, Zhaoji, Qin, Li, Wang, Yuanzheng, Liu, Haobin, Wang, Linxi, Liu, Peilan, Ran, Minyuan, Fang, Chuanying, Zhou, Tao, Shen, Wentao, Valli, Adrián, Cui, Hongguang
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/344612
Acceso en línea:http://hdl.handle.net/10261/344612
Access Level:acceso abierto
Palabra clave:Potyviridae
Potyvirus
P1
HCPro
Zn finger motif
RNA silencing suppression
Host fitness
Telosma mosaic virus
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spelling A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic VirusGou, BeiDai, ZhaojiQin, LiWang, YuanzhengLiu, HaobinWang, LinxiLiu, PeilanRan, MinyuanFang, ChuanyingZhou, TaoShen, WentaoValli, AdriánCui, HongguangPotyviridaePotyvirusP1HCProZn finger motifRNA silencing suppressionHost fitnessTelosma mosaic virusP1 is the first protein translated from the genomes of most viruses in the family Potyviridae, and it contains a C-terminal serine-protease domain that cis-cleaves the junction between P1 and HCPro in most cases. Intriguingly, P1 is the most divergent among all mature viral factors, and its roles during viral infection are still far from understood. In this study, we found that telosma mosaic virus (TelMV, genus Potyvirus) in passion fruit, unlike TelMV isolates present in other hosts, has two stretches at the P1 N terminus, named N1 and N2, with N1 harboring a Zn finger motif. Further analysis revealed that at least 14 different potyviruses, mostly belonging to the bean common mosaic virus subgroup, encode a domain equivalent to N1. Using the newly developed TelMV infectious cDNA clones from passion fruit, we demonstrated that N1, but not N2, is crucial for viral infection in both Nicotiana benthamiana and passion fruit. The regulatory effects of N1 domain on P1 cis cleavage, as well as the accumulation and RNA silencing suppression (RSS) activity of its cognate HCPro, were comprehensively investigated. We found that N1 deletion decreases HCPro abundance at the posttranslational level, likely by impairing P1 cis cleavage, thus reducing HCPro-mediated RSS activity. Remarkably, disruption of the Zn finger motif in N1 did not impair P1 cis cleavage and HCPro accumulation but severely debilitated TelMV fitness. Therefore, our results suggest that the Zn finger motif in P1s plays a critical role in viral infection that is independent of P1 protease activity and self-release, as well as HCPro accumulation and silencing suppression.This work is supported by grants from the Hainan Provincial National Science Foundation (grant nos. 2019RC010 and 322CXTD505), Sanya Yazhou Bay Sci-Tech City (SYND-2022-32 and SYND-2022-02), the National Natural Science Foundation of China (32060603), and the Central Public Interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (19CXTD-33). We thank Fangfang Li (Chinese Academy of Agricultural Sciences) for providing pCHF3-35S-GFP and P19-expressing plasmidPeer reviewedAmerican Society for MicrobiologyNational Natural Science Foundation of ChinaChinese Academy of Agricultural SciencesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420232024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501application/pdfhttp://hdl.handle.net/10261/344612reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1128/jvi.01444-22Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3446122026-05-22T06:33:51Z
dc.title.none.fl_str_mv A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
title A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
spellingShingle A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
Gou, Bei
Potyviridae
Potyvirus
P1
HCPro
Zn finger motif
RNA silencing suppression
Host fitness
Telosma mosaic virus
title_short A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
title_full A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
title_fullStr A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
title_full_unstemmed A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
title_sort A Zinc Finger Motif in the P1 N Terminus, Highly Conserved in a Subset of Potyviruses, Is Associated with the Host Range and Fitness of Telosma Mosaic Virus
dc.creator.none.fl_str_mv Gou, Bei
Dai, Zhaoji
Qin, Li
Wang, Yuanzheng
Liu, Haobin
Wang, Linxi
Liu, Peilan
Ran, Minyuan
Fang, Chuanying
Zhou, Tao
Shen, Wentao
Valli, Adrián
Cui, Hongguang
author Gou, Bei
author_facet Gou, Bei
Dai, Zhaoji
Qin, Li
Wang, Yuanzheng
Liu, Haobin
Wang, Linxi
Liu, Peilan
Ran, Minyuan
Fang, Chuanying
Zhou, Tao
Shen, Wentao
Valli, Adrián
Cui, Hongguang
author_role author
author2 Dai, Zhaoji
Qin, Li
Wang, Yuanzheng
Liu, Haobin
Wang, Linxi
Liu, Peilan
Ran, Minyuan
Fang, Chuanying
Zhou, Tao
Shen, Wentao
Valli, Adrián
Cui, Hongguang
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Natural Science Foundation of China
Chinese Academy of Agricultural Sciences
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Potyviridae
Potyvirus
P1
HCPro
Zn finger motif
RNA silencing suppression
Host fitness
Telosma mosaic virus
topic Potyviridae
Potyvirus
P1
HCPro
Zn finger motif
RNA silencing suppression
Host fitness
Telosma mosaic virus
description P1 is the first protein translated from the genomes of most viruses in the family Potyviridae, and it contains a C-terminal serine-protease domain that cis-cleaves the junction between P1 and HCPro in most cases. Intriguingly, P1 is the most divergent among all mature viral factors, and its roles during viral infection are still far from understood. In this study, we found that telosma mosaic virus (TelMV, genus Potyvirus) in passion fruit, unlike TelMV isolates present in other hosts, has two stretches at the P1 N terminus, named N1 and N2, with N1 harboring a Zn finger motif. Further analysis revealed that at least 14 different potyviruses, mostly belonging to the bean common mosaic virus subgroup, encode a domain equivalent to N1. Using the newly developed TelMV infectious cDNA clones from passion fruit, we demonstrated that N1, but not N2, is crucial for viral infection in both Nicotiana benthamiana and passion fruit. The regulatory effects of N1 domain on P1 cis cleavage, as well as the accumulation and RNA silencing suppression (RSS) activity of its cognate HCPro, were comprehensively investigated. We found that N1 deletion decreases HCPro abundance at the posttranslational level, likely by impairing P1 cis cleavage, thus reducing HCPro-mediated RSS activity. Remarkably, disruption of the Zn finger motif in N1 did not impair P1 cis cleavage and HCPro accumulation but severely debilitated TelMV fitness. Therefore, our results suggest that the Zn finger motif in P1s plays a critical role in viral infection that is independent of P1 protease activity and self-release, as well as HCPro accumulation and silencing suppression.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/344612
url http://hdl.handle.net/10261/344612
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1128/jvi.01444-22
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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