Glycopeptide-resistant Enterococcus faecium. Analysis of the resistance genotype, virulence and genetic lines [Enterococcus faecium resistente a glucopéptidos. Análisis del genotipo de resistencia, virulencia y líneas genéticas]
Objective: The objective of this study was to analyse the genotypic characteristics of all Enterococcus isolates with acquired vancomycin resistance (VRE) recovered in the Hospital Clinic (Barcelona, Spain) in a period of three years and two months. Methods: All VRE isolated in the referred Hospital...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión enviada para evaluación y publicación |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universidad de La Rioja (UR) |
| Repositorio: | RIUR. Repositorio Institucional de la Universidad de La Rioja |
| OAI Identifier: | oai:portal.dialnet.es:doc/5bbc68a1b750603269e80c7d |
| Acceso en línea: | https://investigacion.unirioja.es/documentos/5bbc68a1b750603269e80c7d |
| Access Level: | acceso abierto |
| Palabra clave: | Multi-locus-sequence-typing vanA vanB2 Vancomycin-resistant-Enterococcus faecium |
| Sumario: | Objective: The objective of this study was to analyse the genotypic characteristics of all Enterococcus isolates with acquired vancomycin resistance (VRE) recovered in the Hospital Clinic (Barcelona, Spain) in a period of three years and two months. Methods: All VRE isolated in the referred Hospital in the period January 2004-March 2007 were included in the study. The vancomycin resistance mechanism was investigated, as well as other antibiotic resistance mechanisms. Isolates were also typed by pulsed-field-gel-electrophoresis (PFGE) and multi-locus-sequence-typing (MLST). Results: Thirty-nine VRE were recovered, all being identified as E. faecium, representing 2% of total enterococci obtained in that period. Thirty-eight of them carried the vanA gene, and one isolate the vanB2 gene. The 39 VRE were classified into 13 different pulsotypes (A-M), with one main pulsotype, A, which included 13 isolates. The sequence type was identified by MLST in 24 VRE (with unrelated or closely-related PFGE patterns), and they were ascribed to the clonal complex CC17, but two classified as CC9. All VRE showed a multiresistance phenotype, including, in most cases ampicillin, ciprofloxacin, erythromycin, streptomycin, gentamicin, kanamycin and chloramphenicol, harbouring multiple antibiotic resistance genes. The presence of esp and/or hyl genes was identified in 37 VRE. Conclusion: All VRE, but one, showed the vanA genotype and they were mostly ascribed to the high-risk clonal complex CC17. © 2012 Elsevier España, S.L. All rights reserved. |
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