Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time

Treatments with homologous recombinant follicle-stimulating and luteinizing hormones (rFsh and rLh, respectively) are known to enhance spermatogenesis and sperm production in sole, but the response can be highly variable depending on the dose, duration and time of the year of the rFsh treatment. To...

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Autores: Chauvigné, François, Lleberia, Jessica, Vilafranca, Carlos, Rosado, Diogo, Martins, Micaela, Silva, Frederico, González-López, Wendy, Ramos-Júdez, Sandra, Duncan, Neil, Giménez, Ignacio, Blanquet, Isidro, Cerdà, Joan
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/1574
Acesso em linha:http://hdl.handle.net/20.500.12327/1574
https://doi.org/10.1016/j.aquaculture.2021.737844
Access Level:acceso abierto
Palavra-chave:637
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spelling Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping timeChauvigné, FrançoisLleberia, JessicaVilafranca, CarlosRosado, DiogoMartins, MicaelaSilva, FredericoGonzález-López, WendyRamos-Júdez, SandraDuncan, NeilGiménez, IgnacioBlanquet, IsidroCerdà, Joan637Treatments with homologous recombinant follicle-stimulating and luteinizing hormones (rFsh and rLh, respectively) are known to enhance spermatogenesis and sperm production in sole, but the response can be highly variable depending on the dose, duration and time of the year of the rFsh treatment. To further investigate the physiological effects of rFsh and rLh on sperm production in sole, here we examined the pattern of spermiation of F1 males, of approximately 450 g, treated with rFsh and rLh under controlled temperature. In an initial trial at 12 °C, males were weekly injected intramuscularly with 18 μg kg−1 rFsh over five weeks and subsequently treated with a single injection of 18 μg kg−1 rLh. Histological analysis indicated that the rFsh+rLh treatment increased gonad weight and stimulated spermatogenesis, and also enlarged the size of the seminiferous and efferent duct (ED) tubules, resulting in a doubling of sperm production with respect to the controls. Sperm counts in the ED and sequential stripping of males at 24, 48 and 72 h post rLh injection further revealed that only one batch of spermatids is recruited into spermatozoa (Spz) differentiation after a single rLh induction. A peak of sperm accumulation in the ED occurs at 48 h, coinciding with the upregulation of genes potentially involved in Spz maturation. In a second experiment, we tested the effect of two rFsh doses (10 or 18 μg kg−1) over five weeks as previously, followed by one rLh injection at 12 °C or 17 °C. The results confirmed that spermiation was the highest 48 h after rLh treatment at 12 °C, which was increased in a dose-dependent manner with the dose of rFsh previously supplied (from 0.36 to 0.95 × 109 Spz kg−1). However, sperm production elicited with the low rFsh dose was potentiated by ~3-fold (from 0.36 to 1.06 × 109 Spz kg−1) when the rLh treatment was given at 17 °C. These data suggest that in Senegalese sole sperm collection should be carried out at 48 h after rLh treatment, and that a low dose of rFsh at 12 °C is highly efficient for stimulating sperm production when rLh is administered at a temperature close to that occurring during maximum natural spermiation.info:eu-repo/semantics/acceptedVersionElsevierProducció AnimalAqüicultura2021info:eu-repo/semantics/article26http://hdl.handle.net/20.500.12327/1574https://doi.org/10.1016/j.aquaculture.2021.737844reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAquacultureMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-84013-R/ES/BASE MOLECULAR DE LA ESPERMIOGENESIS EN EL LENGUADO SENEGALES Y DESARROLLO DE METODOS PARA LA ESTIMULACION DE LA PRODUCCION DE ESPERMA EN ACUICULTURA/MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2015-17103/ES/ /Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/15742026-05-29T05:05:01Z
dc.title.none.fl_str_mv Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
title Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
spellingShingle Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
Chauvigné, François
637
title_short Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
title_full Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
title_fullStr Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
title_full_unstemmed Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
title_sort Gonadotropin induction of spermiation in Senegalese sole: Effect of temperature and stripping time
dc.creator.none.fl_str_mv Chauvigné, François
Lleberia, Jessica
Vilafranca, Carlos
Rosado, Diogo
Martins, Micaela
Silva, Frederico
González-López, Wendy
Ramos-Júdez, Sandra
Duncan, Neil
Giménez, Ignacio
Blanquet, Isidro
Cerdà, Joan
author Chauvigné, François
author_facet Chauvigné, François
Lleberia, Jessica
Vilafranca, Carlos
Rosado, Diogo
Martins, Micaela
Silva, Frederico
González-López, Wendy
Ramos-Júdez, Sandra
Duncan, Neil
Giménez, Ignacio
Blanquet, Isidro
Cerdà, Joan
author_role author
author2 Lleberia, Jessica
Vilafranca, Carlos
Rosado, Diogo
Martins, Micaela
Silva, Frederico
González-López, Wendy
Ramos-Júdez, Sandra
Duncan, Neil
Giménez, Ignacio
Blanquet, Isidro
Cerdà, Joan
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
dc.subject.none.fl_str_mv 637
topic 637
description Treatments with homologous recombinant follicle-stimulating and luteinizing hormones (rFsh and rLh, respectively) are known to enhance spermatogenesis and sperm production in sole, but the response can be highly variable depending on the dose, duration and time of the year of the rFsh treatment. To further investigate the physiological effects of rFsh and rLh on sperm production in sole, here we examined the pattern of spermiation of F1 males, of approximately 450 g, treated with rFsh and rLh under controlled temperature. In an initial trial at 12 °C, males were weekly injected intramuscularly with 18 μg kg−1 rFsh over five weeks and subsequently treated with a single injection of 18 μg kg−1 rLh. Histological analysis indicated that the rFsh+rLh treatment increased gonad weight and stimulated spermatogenesis, and also enlarged the size of the seminiferous and efferent duct (ED) tubules, resulting in a doubling of sperm production with respect to the controls. Sperm counts in the ED and sequential stripping of males at 24, 48 and 72 h post rLh injection further revealed that only one batch of spermatids is recruited into spermatozoa (Spz) differentiation after a single rLh induction. A peak of sperm accumulation in the ED occurs at 48 h, coinciding with the upregulation of genes potentially involved in Spz maturation. In a second experiment, we tested the effect of two rFsh doses (10 or 18 μg kg−1) over five weeks as previously, followed by one rLh injection at 12 °C or 17 °C. The results confirmed that spermiation was the highest 48 h after rLh treatment at 12 °C, which was increased in a dose-dependent manner with the dose of rFsh previously supplied (from 0.36 to 0.95 × 109 Spz kg−1). However, sperm production elicited with the low rFsh dose was potentiated by ~3-fold (from 0.36 to 1.06 × 109 Spz kg−1) when the rLh treatment was given at 17 °C. These data suggest that in Senegalese sole sperm collection should be carried out at 48 h after rLh treatment, and that a low dose of rFsh at 12 °C is highly efficient for stimulating sperm production when rLh is administered at a temperature close to that occurring during maximum natural spermiation.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/1574
https://doi.org/10.1016/j.aquaculture.2021.737844
url http://hdl.handle.net/20.500.12327/1574
https://doi.org/10.1016/j.aquaculture.2021.737844
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Aquaculture
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-84013-R/ES/BASE MOLECULAR DE LA ESPERMIOGENESIS EN EL LENGUADO SENEGALES Y DESARROLLO DE METODOS PARA LA ESTIMULACION DE LA PRODUCCION DE ESPERMA EN ACUICULTURA/
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2015-17103/ES/ /
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 26
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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