Cellulase and xylanase production at pilot scale by solid-state fermentation from coffee husk using specialized consortia
Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production sy...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:196245 |
| Acceso en línea: | https://ddd.uab.cat/record/196245 https://dx.doi.org/urn:doi:10.1016/j.biortech.2017.07.076 |
| Access Level: | acceso abierto |
| Palabra clave: | Cellulase Coffee husk Solid-state fermentation Specialized inoculum Xylanase |
| Sumario: | Solid state fermentation is a promising technology however rising concerns related to scale up and reproducibility in a productive process. Coffee husk and a specialized inoculum were used in a 4.5L and then in 50L reactors to assess the reproducibility of a cellulase and hemicellulase production system. Fermentations were consistent in terms of cellulase production and microbial communities. The higher temperatures achieved when operating at 50L generated a shift on the microbial communities and a reduction of nearly 50% on cellulase production at pilot scale. In spite, an overall enzymatic production of 3.1±0.5FPUg⁻¹DM and 48±4Ug⁻¹DM for FPase and Xyl activities was obtained, respectively, with low deviation coefficients of 16 and 19% for FPase and Xyl production. Gaseous emissions assessment revealed an emission factor of 2.6·10⁻³kg volatile organic compounds per Mg of coffee husk and negligible NH₃, CH₄ and N₂O emissions. |
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