Stepwise synthesis of RNA conjugates carrying peptide sequences for RNA interference studies
Oligoribonucleotide conjugates carrying nuclear localization peptide sequences at the 3?-end were prepared stepwise on a single support. The siRNA duplex carrying the nuclear localization peptide sequence at the 3?-end of the passenger strand has similar inhibitory properties as those of unmodified...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2009 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/125054 |
| Acceso en línea: | http://hdl.handle.net/10261/125054 |
| Access Level: | acceso abierto |
| Palabra clave: | Small interfering RNA Cholesterol Amino acid sequence RNA sequence RNA interference RNA synthesis High performance liquid chromatography Peptides Oligoribonucleotides Analysis of Variance Hela cells Tumor necrosis factor SiRNA Oligonucleotide-peptide conjugates Oligonucleotide synthesis Chromatography |
| Sumario: | Oligoribonucleotide conjugates carrying nuclear localization peptide sequences at the 3?-end were prepared stepwise on a single support. The siRNA duplex carrying the nuclear localization peptide sequence at the 3?-end of the passenger strand has similar inhibitory properties as those of unmodified or cholesterol-modified RNA duplexes. |
|---|