Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes

Antioxidant-based treatments are emerging as an interesting approach to possibly counteract obesity fat accumulation complications, since this is accompanied by an increased systemic oxidative stress. The aim of this study was to analyze specific metabolic effects of vitamin C (VC) on epididymal pri...

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Autores: Garcia-Garcia, D.F. (D. F.)|||/items/592baa9a-2828-4782-85ea-545d4766ff91, Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c, Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb, Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b, Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82, Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/28186
Acceso en línea:https://hdl.handle.net/10171/28186
Access Level:acceso abierto
Palabra clave:Adipocytes
Antioxidants
Ascorbic acid
Glucose
Lipid metabolism
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spelling Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytesGarcia-Garcia, D.F. (D. F.)|||/items/592baa9a-2828-4782-85ea-545d4766ff91Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817cMilagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724ebBoque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989bMoreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131AdipocytesAntioxidantsAscorbic acidGlucoseLipid metabolismAntioxidant-based treatments are emerging as an interesting approach to possibly counteract obesity fat accumulation complications, since this is accompanied by an increased systemic oxidative stress. The aim of this study was to analyze specific metabolic effects of vitamin C (VC) on epididymal primary rat adipocytes. Cells were isolated and incubated for 72 h in culture medium, in the absence or presence of 1.6 nM insulin, within a range of VC concentrations (5-1000 microM). Glucose- and lipid-related variables as well as the secretion/expression patterns of several obesity-related genes were assessed. It was observed that VC dose dependently inhibited glucose uptake and lactate production, and also reduced glycerol release in both control and insulin-treated cells. Also, VC caused a dramatic concentration-dependent fall in leptin secretion especially in insulin-stimulated cells. In addition, VC (200 microM) induced Cdkn1a and Casp8, partially inhibited Irs3, and together with insulin drastically reduced Gpdh (listed as Gpd1 in the MGI database) gene expressions. Finally, VC and insulin down-regulatory effects were observed on extracellular and intracellular reactive oxygen species production respectively. In summary, this experimental assay describes a specific effect of VC in isolated rat adipocytes on glucose and fat metabolism, and on the secretion/expression of important obesity-related proteins.European Society of EndocrinologyDadun. Depósito Académico Digital Universidad de Navarra20132013-03-2820102010-01-0120102010-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/28186reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/281862026-06-21T12:47:57Z
dc.title.none.fl_str_mv Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
title Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
spellingShingle Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
Garcia-Garcia, D.F. (D. F.)|||/items/592baa9a-2828-4782-85ea-545d4766ff91
Adipocytes
Antioxidants
Ascorbic acid
Glucose
Lipid metabolism
title_short Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
title_full Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
title_fullStr Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
title_full_unstemmed Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
title_sort Vitamin C inhibits leptin secretion and some glucose/lipid metabolic pathways in primary rat adipocytes
dc.creator.none.fl_str_mv Garcia-Garcia, D.F. (D. F.)|||/items/592baa9a-2828-4782-85ea-545d4766ff91
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author Garcia-Garcia, D.F. (D. F.)|||/items/592baa9a-2828-4782-85ea-545d4766ff91
author_facet Garcia-Garcia, D.F. (D. F.)|||/items/592baa9a-2828-4782-85ea-545d4766ff91
Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author_role author
author2 Campión-Zabalza, J. (Javier)|||/items/e0303c16-4823-4c72-80a7-28a752b6817c
Milagro-Yoldi, F.I. (Fermín Ignacio)|||/items/07cf7af6-1f5f-4720-8c14-5197a7a724eb
Boque, N. (Noemi)|||/items/db72d734-711b-40c8-9066-a04f57a4989b
Moreno-Aliaga, M. J. (María Jesús)|||/items/79b762db-92f6-43e2-ba5a-d94e1a69aa82
Martinez, J.A. (José Alfredo)|||/items/6a3581ea-897b-4439-a95c-19301775e131
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Adipocytes
Antioxidants
Ascorbic acid
Glucose
Lipid metabolism
topic Adipocytes
Antioxidants
Ascorbic acid
Glucose
Lipid metabolism
description Antioxidant-based treatments are emerging as an interesting approach to possibly counteract obesity fat accumulation complications, since this is accompanied by an increased systemic oxidative stress. The aim of this study was to analyze specific metabolic effects of vitamin C (VC) on epididymal primary rat adipocytes. Cells were isolated and incubated for 72 h in culture medium, in the absence or presence of 1.6 nM insulin, within a range of VC concentrations (5-1000 microM). Glucose- and lipid-related variables as well as the secretion/expression patterns of several obesity-related genes were assessed. It was observed that VC dose dependently inhibited glucose uptake and lactate production, and also reduced glycerol release in both control and insulin-treated cells. Also, VC caused a dramatic concentration-dependent fall in leptin secretion especially in insulin-stimulated cells. In addition, VC (200 microM) induced Cdkn1a and Casp8, partially inhibited Irs3, and together with insulin drastically reduced Gpdh (listed as Gpd1 in the MGI database) gene expressions. Finally, VC and insulin down-regulatory effects were observed on extracellular and intracellular reactive oxygen species production respectively. In summary, this experimental assay describes a specific effect of VC in isolated rat adipocytes on glucose and fat metabolism, and on the secretion/expression of important obesity-related proteins.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01
2010
2010-01-01
2013
2013-03-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/28186
url https://hdl.handle.net/10171/28186
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv European Society of Endocrinology
publisher.none.fl_str_mv European Society of Endocrinology
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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