Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas

Papaver rhoeas biotypes displaying multiple herbicide resistance to ALS inhibitors and synthetic auxin herbicides (SAH) are spreading across Europe. In Spain, enhanced metabolism to imazamox was confirmed in one population, while cytochrome-P450 (P450) based metabolism to 2,4-D in another two. The o...

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Autores: Torra Farré, Joel, Menéndez, Julio, Rojano Delgado, Antonia María, Salas, Marisa, Prado, Rafael de
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Universitat de Lleida (UdL)
Repositório:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/72656
Acesso em linha:https://doi.org/10.1016/j.plaphy.2021.01.007
http://hdl.handle.net/10459.1/72656
Access Level:Acceso aberto
Palavra-chave:ALS inhibiting herbicides
Cross-resistance
Enhanced metabolism
Imazamox
P450 inhibitor
Synthetic auxin herbicides
Herbicides
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spelling Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeasTorra Farré, JoelMenéndez, JulioRojano Delgado, Antonia MaríaSalas, MarisaPrado, Rafael deALS inhibiting herbicidesCross-resistanceEnhanced metabolismImazamoxP450 inhibitorSynthetic auxin herbicidesHerbicidesPapaver rhoeas biotypes displaying multiple herbicide resistance to ALS inhibitors and synthetic auxin herbicides (SAH) are spreading across Europe. In Spain, enhanced metabolism to imazamox was confirmed in one population, while cytochrome-P450 (P450) based metabolism to 2,4-D in another two. The objectives of this research were to further confirm the presence of P450 mediated enhanced metabolism and, if so, to confirm whether a putative common P450 is responsible of metabolizing both 2,4-D and imazamox. Metabolism studies were undertaken in five P. rhoeas populations with contrasted HR profiles (herbicide susceptible, only HR to ALS inhibitors, only HR to SAH, or multiple HR to both), and moreover, three different P450 inhibitors were used. The presence of enhanced metabolism to these SoA was confirmed in three more HR P. rhoeas populations. This study provides the first direct evidence that imazamox metabolism in these biotypes is P450-mediated, also in one population without an altered target site. Additionally, it was further confirmed that enhanced metabolism of 2,4-D in biotypes only HR to SAH or multiple HR to ALS inhibitors and SAH involves P450 as well. No metabolism was detected using the three inhibitors in all the herbicide-metabolizing P. rhoeas biotypes, suggesting that a common metabolic system involving P450s is responsible of degrading herbicides affecting both SoAs. Thus, selection pressure with either SAH or imidazolinone ALS inhibitors can select not only for resistance to each of them, but it can also confer cross-resistance between them in P. rhoeas.The authors gratefully acknowledge Corteva Agrisciences for fund-ing the experiments and also the support from the Cathedra University of Lleida-Corteva Agrisciences. The research undertaken at the University of Lleida-Agrotecnio has been supported by the Spanish Government, through project AGL 2017-83325-C4-2-R (AEI/FEDER/UE). Joel Torra acknowledges support from the Spanish Ministry of Science, Innovation and Universities (grant Ramon y Cajal RYC 2018-023866-I).Elsevier2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1016/j.plaphy.2021.01.007http://hdl.handle.net/10459.1/72656reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83325-C4-2-RReproducció del document publicat a https://doi.org/10.1016/j.plaphy.2021.01.007Plant Physiology and Biochemistry, 2021, vol. 160, p. 51-61cc-by (c) Torra et al., 2021info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/726562026-06-24T12:42:17Z
dc.title.none.fl_str_mv Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
title Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
spellingShingle Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
Torra Farré, Joel
ALS inhibiting herbicides
Cross-resistance
Enhanced metabolism
Imazamox
P450 inhibitor
Synthetic auxin herbicides
Herbicides
title_short Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
title_full Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
title_fullStr Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
title_full_unstemmed Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
title_sort Cytochrome P450 metabolism-based herbicide resistance to imazamox and 2,4-D in Papaver rhoeas
dc.creator.none.fl_str_mv Torra Farré, Joel
Menéndez, Julio
Rojano Delgado, Antonia María
Salas, Marisa
Prado, Rafael de
author Torra Farré, Joel
author_facet Torra Farré, Joel
Menéndez, Julio
Rojano Delgado, Antonia María
Salas, Marisa
Prado, Rafael de
author_role author
author2 Menéndez, Julio
Rojano Delgado, Antonia María
Salas, Marisa
Prado, Rafael de
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALS inhibiting herbicides
Cross-resistance
Enhanced metabolism
Imazamox
P450 inhibitor
Synthetic auxin herbicides
Herbicides
topic ALS inhibiting herbicides
Cross-resistance
Enhanced metabolism
Imazamox
P450 inhibitor
Synthetic auxin herbicides
Herbicides
description Papaver rhoeas biotypes displaying multiple herbicide resistance to ALS inhibitors and synthetic auxin herbicides (SAH) are spreading across Europe. In Spain, enhanced metabolism to imazamox was confirmed in one population, while cytochrome-P450 (P450) based metabolism to 2,4-D in another two. The objectives of this research were to further confirm the presence of P450 mediated enhanced metabolism and, if so, to confirm whether a putative common P450 is responsible of metabolizing both 2,4-D and imazamox. Metabolism studies were undertaken in five P. rhoeas populations with contrasted HR profiles (herbicide susceptible, only HR to ALS inhibitors, only HR to SAH, or multiple HR to both), and moreover, three different P450 inhibitors were used. The presence of enhanced metabolism to these SoA was confirmed in three more HR P. rhoeas populations. This study provides the first direct evidence that imazamox metabolism in these biotypes is P450-mediated, also in one population without an altered target site. Additionally, it was further confirmed that enhanced metabolism of 2,4-D in biotypes only HR to SAH or multiple HR to ALS inhibitors and SAH involves P450 as well. No metabolism was detected using the three inhibitors in all the herbicide-metabolizing P. rhoeas biotypes, suggesting that a common metabolic system involving P450s is responsible of degrading herbicides affecting both SoAs. Thus, selection pressure with either SAH or imidazolinone ALS inhibitors can select not only for resistance to each of them, but it can also confer cross-resistance between them in P. rhoeas.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.plaphy.2021.01.007
http://hdl.handle.net/10459.1/72656
url https://doi.org/10.1016/j.plaphy.2021.01.007
http://hdl.handle.net/10459.1/72656
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83325-C4-2-R
Reproducció del document publicat a https://doi.org/10.1016/j.plaphy.2021.01.007
Plant Physiology and Biochemistry, 2021, vol. 160, p. 51-61
dc.rights.none.fl_str_mv cc-by (c) Torra et al., 2021
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Torra et al., 2021
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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